FastQCFastQC Report
Wed 25 May 2016
SRR1779774_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779774_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252060
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT1300.053.84401346
GTATCAC200.006946863652.49791757
TTCCGGT352.0832846E-549.99801643
GCCGAAA1150.048.6937270
GCCGTAT1100.047.7253854
CTTCCGA751.4551915E-1146.66481818
ATCTCGG1150.045.65036442
AGATCTC1000.045.49819640
TCTCGTT1000.045.49819643
TCTCGGT1550.045.159543
TCGGTGG1650.042.4225645
TGGTCGC1350.041.47983649
GTAGATC953.6379788E-1240.52470838
GATCTCG1400.039.99841341
TCGTGTC459.1836126E-538.88734434
CTCGGTG1800.038.88734444
CGTATCA1100.038.180356
CTGTGTA654.8515176E-737.6908134
CGTGTAC753.5246558E-837.33185235
CCGTGTA852.562956E-937.05735434