##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779774_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	252060
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.310485598666983	34.0	31.0	34.0	21.0	34.0
2	28.67101880504642	34.0	23.0	34.0	12.0	34.0
3	27.45838292470047	33.0	23.0	34.0	11.0	34.0
4	29.99337459335079	34.0	31.0	34.0	21.0	34.0
5	29.588891533761803	34.0	31.0	34.0	11.0	34.0
6	32.13428152027295	37.0	31.0	38.0	12.0	38.0
7	33.17226057287947	37.0	34.0	38.0	21.0	38.0
8	32.37145917638657	37.0	31.0	38.0	12.0	38.0
9	33.715567721970956	37.0	34.0	38.0	21.0	38.0
10-11	33.538613822105845	38.0	34.0	38.0	21.0	38.0
12-13	32.41582758073474	37.5	30.0	38.0	16.0	38.0
14-15	32.54087122113782	37.0	32.5	38.0	16.0	38.0
16-17	32.17952273268269	36.5	30.0	38.0	16.0	38.0
18-19	32.813925255891455	37.0	32.0	38.0	16.0	38.0
20-21	31.659727842577166	36.5	28.5	38.0	16.0	38.0
22-23	31.63316670633976	36.5	28.5	38.0	16.0	38.0
24-25	32.91217368880425	37.0	32.5	38.0	16.5	38.0
26-27	31.43203007220503	37.0	28.0	38.0	11.0	38.0
28-29	33.60992819170039	37.5	34.0	38.0	21.0	38.0
30-31	34.0581746409585	38.0	34.5	38.0	21.5	38.0
32-33	34.1151947948901	38.0	35.0	38.0	22.0	38.0
34-35	34.18909783384908	38.0	35.0	38.0	22.0	38.0
36-37	32.045985082916765	37.0	30.0	38.0	16.0	38.0
38-39	30.181768229786556	35.0	24.0	38.0	11.0	38.0
40-41	31.547437118146473	36.5	28.5	38.0	11.0	38.0
42-43	32.60251725779577	37.0	30.5	38.0	16.0	38.0
44-45	34.230119812743	38.0	35.5	38.0	23.0	38.0
46-47	32.7536935650242	37.5	31.0	38.0	17.0	38.0
48-49	32.256688883599146	37.0	30.0	38.0	16.0	38.0
50-51	29.98416051733714	35.0	23.5	38.0	11.0	38.0
52-53	33.47498214710783	37.0	34.0	38.0	21.5	38.0
54-55	32.5238455129731	37.0	30.5	38.0	16.0	38.0
56-57	32.934257716416724	37.5	31.0	38.0	17.0	38.0
58-59	34.0426941997937	38.0	35.0	38.0	22.0	38.0
60-61	34.45733952233595	38.0	36.0	38.0	23.0	38.0
62-63	34.35262437514878	38.0	36.0	38.0	23.0	38.0
64-65	34.05411608347219	38.0	35.0	38.0	22.5	38.0
66-67	31.919350551456002	37.0	28.5	38.0	11.0	38.0
68-69	32.05828969293026	37.0	29.0	38.0	11.0	38.0
70-71	31.649994049035946	37.0	28.0	38.0	11.0	38.0
72-73	30.378338490835514	35.5	24.5	38.0	11.0	38.0
74-75	32.692769578671744	37.0	31.0	38.0	16.0	38.0
76	33.131389351741646	37.0	34.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	1.0
5	2.0
6	2.0
7	2.0
8	2.0
9	1.0
10	0.0
11	9.0
12	53.0
13	285.0
14	801.0
15	1426.0
16	2272.0
17	3513.0
18	4539.0
19	4667.0
20	4213.0
21	3437.0
22	2865.0
23	2459.0
24	2668.0
25	2818.0
26	3355.0
27	3908.0
28	4885.0
29	6358.0
30	8009.0
31	10702.0
32	14315.0
33	19894.0
34	28545.0
35	39727.0
36	47980.0
37	28345.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.239546139807985	18.21709116876934	10.942235975561374	28.6011267158613
2	23.23216694437832	18.23018328969293	35.303895897802114	23.233753868126637
3	20.802189954772672	19.000238038562248	34.196223121479015	26.001348885186065
4	14.630246766642863	18.829247004681427	34.58938348012378	31.95112274855193
5	14.043878441640878	30.534396572244702	38.65468539236689	16.76703959374752
6	27.13837975085297	33.65944616361184	18.212330397524397	20.98984368801079
7	27.727921923351584	27.69856383400778	21.42148694755217	23.15202729508847
8	24.26662540566704	31.44127845620383	19.227625826211845	25.064470311917287
9	24.2759430593687	17.201609191754084	20.757224699665148	37.76522304921207
10-11	24.711268751165438	24.232794156738123	27.361526040364847	23.694411051731596
12-13	25.14054806803439	20.94791092208261	28.34349670103829	25.568044308844705
14-15	23.42216350464583	22.94726526855356	24.712363223753638	28.918208003046963
16-17	23.764672275375574	24.315359940646346	27.184648986207023	24.735318797771065
18-19	22.128105280489425	24.493305904967457	27.854537086813952	25.524051727729162
20-21	23.119984923775803	24.114023864075936	28.393886194344432	24.37210501780383
22-23	23.772512938926678	23.34719965721019	28.750106625881028	24.1301807779821
24-25	22.48169043141549	25.607409523356107	25.268196497575918	26.642703547652484
26-27	22.164849268709744	24.76964307322424	28.51010396561061	24.555403692455403
28-29	22.618943710028088	25.86351229111453	27.264612064177236	24.252931934680145
30-31	22.754765414073766	25.38915812505827	27.09258660466851	24.763489856199453
32-33	22.332672088871256	25.734179726244793	26.96726839912716	24.965879785756794
34-35	23.073199761951994	25.386232890299542	27.114064669708394	24.42650267804007
36-37	23.23025132067836	23.753166553262567	30.216803512022487	22.799778614036583
38-39	22.973844215872006	22.811573215366153	32.71645225602317	21.49813031273867
40-41	22.749634990160605	23.350909350599885	26.795888084809246	27.103567574430265
42-43	20.947172348946957	24.103818405073486	27.750039177879476	27.19897006810008
44-45	21.088516343019922	23.817742149855484	29.519598332867155	25.574143174257436
46-47	21.972295344403822	22.829263067269984	30.4951567437607	24.703284844565495
48-49	21.891688157111684	23.394366197183096	30.904979170799447	23.808966474905773
50-51	22.498135305413168	22.17141700918858	32.963832859886054	22.366614825512197
52-53	19.782818283891494	24.775339520010156	29.738302776863044	25.703539419235298
54-55	20.65448110868211	24.11931108086984	29.666887524945746	25.559320285502306
56-57	20.774086299652453	24.26860330408011	29.226508815641218	25.730801580626224
58-59	19.501328112136704	24.83480427455718	29.33709449929478	26.32677311401134
60-61	19.582501815123248	24.358160516407523	29.30620633289559	26.753131335573638
62-63	19.62332795740519	25.00580243166521	28.76875375670255	26.602115854227048
64-65	20.131601394966854	25.176850533031274	27.52024407952422	27.171303992477654
66-67	19.402532067431867	24.056029232643116	24.886628287581285	31.654810412343732
68-69	19.49764776612188	24.565615519386334	25.16582703193919	30.770909682552595
70-71	19.669308364494785	24.40631230224462	24.8036580406471	31.120721292613496
72-73	19.68811805570709	23.926356027859498	23.175316057694786	33.21020985873863
74-75	20.502321964534744	26.406320980601034	24.782830356735484	28.308526698128738
76	21.263013394274108	25.738351425125373	25.284866374658794	27.713768805941726
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.5
4	1.0
5	1.0
6	1.5
7	1.0
8	0.0
9	0.0
10	1.0
11	2.5
12	3.0
13	2.5
14	3.5
15	3.5
16	5.5
17	9.0
18	14.0
19	21.0
20	37.0
21	51.0
22	67.5
23	99.0
24	190.0
25	266.0
26	337.5
27	527.0
28	820.0
29	995.0
30	1217.0
31	1730.5
32	2490.5
33	2959.0
34	3520.0
35	4709.5
36	6139.0
37	6940.0
38	7812.0
39	9741.0
40	11792.5
41	13697.0
42	14607.0
43	15309.0
44	16377.0
45	17050.0
46	17357.0
47	17418.0
48	16937.5
49	15576.5
50	14757.0
51	13726.5
52	11654.0
53	9725.0
54	8838.0
55	8081.0
56	6503.0
57	5175.0
58	4668.0
59	4202.0
60	3471.5
61	3065.0
62	2923.0
63	2742.5
64	2487.0
65	2306.0
66	2133.0
67	2066.0
68	1934.5
69	1775.0
70	1683.0
71	1619.0
72	1552.5
73	1417.0
74	1218.5
75	1089.0
76	968.0
77	771.0
78	622.5
79	550.0
80	433.0
81	280.0
82	201.0
83	158.0
84	113.5
85	65.5
86	44.5
87	27.0
88	23.0
89	14.0
90	7.5
91	4.0
92	2.0
93	1.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.00238038562247084
9	0.003173847496627787
10-11	0.00357057843370626
12-13	0.004364040307863208
14-15	0.00238038562247084
16-17	0.004562405776402444
18-19	0.003768943902245497
20-21	0.0029754820280885504
22-23	0.00416567483932397
24-25	0.00238038562247084
26-27	0.002182020153931604
28-29	0.003173847496627787
30-31	0.003768943902245497
32-33	0.003967309370784734
34-35	0.003967309370784734
36-37	0.004562405776402444
38-39	0.004959136713480917
40-41	0.00476077124494168
42-43	0.00178528921685313
44-45	0.00416567483932397
46-47	0.003372212965167024
48-49	0.003967309370784734
50-51	0.003173847496627787
52-53	0.005157502182020154
54-55	0.005157502182020154
56-57	0.003173847496627787
58-59	0.00416567483932397
60-61	0.004364040307863208
62-63	0.00416567483932397
64-65	0.004364040307863208
66-67	0.005157502182020154
68-69	0.02598587637864001
70-71	0.004959136713480917
72-73	0.003768943902245497
74-75	0.00416567483932397
76	0.00476077124494168
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	252060.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.8922281190691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.69021233448237	87.93186820602415
2	2.681789352483545	4.928711978914172
3	0.7249313821249255	1.9984667981068713
4	0.3390408227832278	1.2462086651549302
5	0.1939881875082884	0.8913003389458194
6	0.11680780970749598	0.6440237937438023
7	0.06649264762061584	0.4277110280376156
8	0.039997650923544646	0.29403786103146085
9	0.032487258260728465	0.26867938923521756
>10	0.11425255410523383	1.368991940805954
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.934618741569468E-4	0.0
4	0.0	0.0	0.0	7.934618741569468E-4	0.0
5	0.0	0.0	0.0	0.0015869237483138936	0.0
6	0.0	0.0	0.0	0.0015869237483138936	0.0
7	0.0	0.0	0.0	0.001983654685392367	0.0
8	0.0	0.0	0.0	0.0035705784337062606	0.0
9	0.0	0.0	0.0	0.006347694993255574	0.0
10	0.0	0.0	0.0	0.006744425930334048	0.0
11	0.0	0.0	0.0	0.007141156867412521	0.0
12	0.0	0.0	0.0	0.007141156867412521	0.0
13	0.0	0.0	0.0	0.007141156867412521	0.0
14	0.0	0.0	0.0	0.007141156867412521	0.0
15	0.0	0.0	0.0	0.007537887804490994	0.0
16	0.0	0.0	0.0	0.007537887804490994	0.0
17	0.0	0.0	0.0	0.007934618741569468	0.0
18	0.0	0.0	0.0	0.009124811552804887	0.0
19	0.0	0.0	0.0	0.011108466238197254	0.0
20	0.0	0.0	0.0	0.011901928112354201	0.0
21	0.0	0.0	0.0	0.012298659049432675	0.0
22	0.0	0.0	0.0	0.017059430294374355	0.0
23	0.0	0.0	0.0	0.01904308497976672	0.0
24	0.0	0.0	0.0	0.023010394350551456	0.0
25	0.0	0.0	0.0	0.024200587161786875	0.0
26	0.0	0.0	0.0	0.027771165595493138	0.0
27	0.0	0.0	0.0	0.067047528366262	0.0
28	0.0	0.0	0.0	0.09164484646512736	0.0
29	0.0	0.0	0.0	0.10751408394826628	0.0
30	0.0	0.0	0.0	0.141632944537015	0.0
31	0.0	0.0	0.0	0.16067602951678173	0.0
32	0.0	0.0	0.0	0.21106085852574782	0.0
33	0.0	0.0	0.0	0.25232087598190905	0.0
34	0.0	0.0	3.967309370784734E-4	0.3027057049908752	0.0
35	0.0	0.0	3.967309370784734E-4	0.4126001745616123	0.0
36	0.0	0.0	3.967309370784734E-4	0.5046417519638181	0.0
37	0.0	0.0	3.967309370784734E-4	0.7652939776243751	0.0
38	0.0	0.0	3.967309370784734E-4	1.0826787272871539	0.0
39	0.0	0.0	3.967309370784734E-4	1.4444973419027216	0.0
40	0.0	0.0	3.967309370784734E-4	1.9229548520193604	0.0
41	0.0	0.0	3.967309370784734E-4	2.6454018884392605	3.967309370784734E-4
42	0.0	0.0	3.967309370784734E-4	3.390065857335555	3.967309370784734E-4
43	0.0	0.0	3.967309370784734E-4	4.19701658335317	3.967309370784734E-4
44	0.0	0.0	3.967309370784734E-4	4.925414583829247	3.967309370784734E-4
45	0.0	0.0	3.967309370784734E-4	5.385622470840276	3.967309370784734E-4
46	0.0	0.0	3.967309370784734E-4	5.942235975561374	3.967309370784734E-4
47	0.0	0.0	3.967309370784734E-4	6.551614694913909	7.934618741569468E-4
48	0.0	0.0	3.967309370784734E-4	7.036023169086725	7.934618741569468E-4
49	0.0	0.0	3.967309370784734E-4	7.472823930810125	7.934618741569468E-4
50	0.0	0.0	3.967309370784734E-4	8.114734587003095	7.934618741569468E-4
51	0.0	0.0	3.967309370784734E-4	8.860985479647702	7.934618741569468E-4
52	0.0	0.0	3.967309370784734E-4	9.56597635483615	7.934618741569468E-4
53	0.0	0.0	3.967309370784734E-4	10.651035467745775	7.934618741569468E-4
54	0.0	0.0	3.967309370784734E-4	11.637705308259939	7.934618741569468E-4
55	0.0	0.0	3.967309370784734E-4	12.132428786796794	7.934618741569468E-4
56	0.0	0.0	3.967309370784734E-4	12.633499960326906	7.934618741569468E-4
57	0.0	0.0	3.967309370784734E-4	13.04570340395144	7.934618741569468E-4
58	0.0	0.0	3.967309370784734E-4	13.442831071966992	7.934618741569468E-4
59	0.0	0.0	3.967309370784734E-4	13.899468380544315	7.934618741569468E-4
60	0.0	0.0	3.967309370784734E-4	14.21010870427676	7.934618741569468E-4
61	0.0	0.0	3.967309370784734E-4	14.54494961517099	7.934618741569468E-4
62	0.0	0.0	3.967309370784734E-4	14.79965087677537	7.934618741569468E-4
63	0.0	0.0	3.967309370784734E-4	15.179719114496548	7.934618741569468E-4
64	0.0	0.0	3.967309370784734E-4	15.613742759660399	7.934618741569468E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGGT	130	0.0	53.844013	46
GTATCAC	20	0.0069468636	52.497917	57
TTCCGGT	35	2.0832846E-5	49.998016	43
GCCGAAA	115	0.0	48.69372	70
GCCGTAT	110	0.0	47.72538	54
CTTCCGA	75	1.4551915E-11	46.664818	18
ATCTCGG	115	0.0	45.650364	42
AGATCTC	100	0.0	45.498196	40
TCTCGTT	100	0.0	45.498196	43
TCTCGGT	155	0.0	45.1595	43
TCGGTGG	165	0.0	42.42256	45
TGGTCGC	135	0.0	41.479836	49
GTAGATC	95	3.6379788E-12	40.524708	38
GATCTCG	140	0.0	39.998413	41
TCGTGTC	45	9.1836126E-5	38.887344	34
CTCGGTG	180	0.0	38.887344	44
CGTATCA	110	0.0	38.1803	56
CTGTGTA	65	4.8515176E-7	37.69081	34
CGTGTAC	75	3.5246558E-8	37.331852	35
CCGTGTA	85	2.562956E-9	37.057354	34
>>END_MODULE