FastQCFastQC Report
Wed 25 May 2016
SRR1779774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252060
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT174816.935253511068794No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG21920.8696342140760137No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC13980.5546298500357058No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT8010.31778148059985717RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5750.22812028882012217No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC4360.1729746885662144RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT4090.1622629532650956No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2920.11584543362691423No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAG150.002222884270.024
TCGATAT150.002222884270.034
ATCGTAT150.002222884270.039
ATATCGT150.002222884270.037
CGCTACA252.3874453E-455.99999624
TCTACAG200.006945772652.52
TCTAATG200.006945772652.519
GAAAAGT200.006945772652.525
CTACATG200.006945772652.54
CCGTCTT27550.050.94373747
TCTCGTA26250.050.838
TCGTATG27650.050.75949540
CTCGTAT27450.050.74681539
GCCGTCT27600.050.72463646
CGTCTTC27800.050.6115148
ATCTCGT26350.050.60721237
TATGCCG27750.050.57657643
CGTATGC27700.050.54151541
TGCCGTC27800.050.3597145
CTGCTTG27950.050.33989354