Basic Statistics
Measure | Value |
---|---|
Filename | SRR1779774_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 252060 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT | 17481 | 6.935253511068794 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG | 2192 | 0.8696342140760137 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC | 1398 | 0.5546298500357058 | No Hit |
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT | 801 | 0.31778148059985717 | RNA PCR Primer, Index 42 (95% over 22bp) |
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC | 575 | 0.22812028882012217 | No Hit |
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC | 436 | 0.1729746885662144 | RNA PCR Primer, Index 32 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT | 409 | 0.1622629532650956 | No Hit |
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC | 292 | 0.11584543362691423 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGAAAAG | 15 | 0.0022228842 | 70.0 | 24 |
TCGATAT | 15 | 0.0022228842 | 70.0 | 34 |
ATCGTAT | 15 | 0.0022228842 | 70.0 | 39 |
ATATCGT | 15 | 0.0022228842 | 70.0 | 37 |
CGCTACA | 25 | 2.3874453E-4 | 55.999996 | 24 |
TCTACAG | 20 | 0.0069457726 | 52.5 | 2 |
TCTAATG | 20 | 0.0069457726 | 52.5 | 19 |
GAAAAGT | 20 | 0.0069457726 | 52.5 | 25 |
CTACATG | 20 | 0.0069457726 | 52.5 | 4 |
CCGTCTT | 2755 | 0.0 | 50.943737 | 47 |
TCTCGTA | 2625 | 0.0 | 50.8 | 38 |
TCGTATG | 2765 | 0.0 | 50.759495 | 40 |
CTCGTAT | 2745 | 0.0 | 50.746815 | 39 |
GCCGTCT | 2760 | 0.0 | 50.724636 | 46 |
CGTCTTC | 2780 | 0.0 | 50.61151 | 48 |
ATCTCGT | 2635 | 0.0 | 50.607212 | 37 |
TATGCCG | 2775 | 0.0 | 50.576576 | 43 |
CGTATGC | 2770 | 0.0 | 50.541515 | 41 |
TGCCGTC | 2780 | 0.0 | 50.35971 | 45 |
CTGCTTG | 2795 | 0.0 | 50.339893 | 54 |