##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779773_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81090
Sequences flagged as poor quality	0
Sequence length	76
%GC	55
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.384498705142434	31.0	12.0	34.0	12.0	34.0
2	25.202614379084967	24.0	21.0	34.0	12.0	34.0
3	24.60673325934147	23.0	11.0	34.0	11.0	34.0
4	25.436021704279195	31.0	21.0	34.0	11.0	34.0
5	25.498433838944383	31.0	21.0	34.0	11.0	34.0
6	26.82446664200271	31.0	12.0	37.0	11.0	38.0
7	27.95791096312739	34.0	21.0	37.0	12.0	38.0
8	27.04931557528672	31.0	12.0	37.0	11.0	38.0
9	28.61749907510174	34.0	21.0	38.0	12.0	38.0
10-11	27.910611666050066	34.0	21.0	38.0	11.0	38.0
12-13	27.835269453693428	31.0	21.0	37.5	11.0	38.0
14-15	26.98787150080158	31.0	11.5	37.0	11.0	38.0
16-17	26.933068195831794	30.5	16.0	37.5	11.0	38.0
18-19	27.429072635343445	31.0	21.0	37.0	11.0	38.0
20-21	26.472992970773213	29.0	16.0	37.0	11.0	38.0
22-23	26.618578123073128	30.5	16.0	37.5	11.0	38.0
24-25	27.082907880133185	32.0	16.5	37.0	11.0	38.0
26-27	26.020563571340485	29.0	11.0	37.0	11.0	38.0
28-29	27.219990134418545	32.0	11.0	37.5	11.0	38.0
30-31	27.685953878406707	34.0	11.0	38.0	11.0	38.0
32-33	27.870471081514367	34.0	11.0	38.0	11.0	38.0
34-35	27.920649895178197	34.0	11.0	38.0	11.0	38.0
36-37	26.723029966703663	29.5	16.0	37.0	11.0	38.0
38-39	25.963534344555434	26.5	16.0	36.5	11.0	38.0
40-41	26.020606733259342	27.0	11.0	37.0	11.0	38.0
42-43	26.73097175977309	29.0	11.0	37.0	11.0	38.0
44-45	27.894185472931312	34.0	21.0	38.0	11.0	38.0
46-47	27.040128252558887	30.5	15.0	37.5	11.0	38.0
48-49	26.366302873350598	29.0	11.0	37.0	11.0	38.0
50-51	25.08475767665557	26.0	11.0	36.0	11.0	38.0
52-53	26.86778887655691	32.0	11.0	37.0	11.0	38.0
54-55	26.36815266987298	28.5	11.0	37.0	11.0	38.0
56-57	26.766500184979652	30.5	11.0	37.5	11.0	38.0
58-59	27.368097175977308	33.5	11.0	38.0	11.0	38.0
60-61	27.920736219015907	34.0	14.5	38.0	11.0	38.0
62-63	27.907806141324457	34.0	14.5	38.0	11.0	38.0
64-65	27.731853496115427	34.0	15.0	38.0	11.0	38.0
66-67	26.499901344185474	28.0	11.0	37.0	11.0	38.0
68-69	26.38406708595388	28.5	11.0	37.0	11.0	38.0
70-71	26.20356394129979	27.5	11.0	37.0	11.0	38.0
72-73	25.480922431865828	26.5	11.0	36.5	11.0	38.0
74-75	26.290048094709583	29.0	11.0	37.0	11.0	38.0
76	26.037748181033418	27.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	0.0
5	4.0
6	2.0
7	1.0
8	1.0
9	1.0
10	2.0
11	19.0
12	85.0
13	394.0
14	1014.0
15	1809.0
16	2987.0
17	4558.0
18	5962.0
19	6086.0
20	5140.0
21	3879.0
22	2785.0
23	1976.0
24	1644.0
25	1435.0
26	1325.0
27	1395.0
28	1544.0
29	1756.0
30	2055.0
31	2559.0
32	3188.0
33	4158.0
34	5452.0
35	6912.0
36	7191.0
37	3767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.53052164261931	15.823159452460228	15.33481317055124	36.31150573436922
2	16.974966087063756	14.735479097299297	45.01171537797509	23.277839437661857
3	17.97755580219509	14.031323221112343	42.926378098409174	25.06474287828339
4	14.729858053695413	21.048996756570105	25.6354286137112	38.58571657602328
5	14.884696016771489	22.311012455296584	41.63275373042299	21.17153779750894
6	25.60611666050068	31.092613145887288	16.666666666666664	26.634603526945373
7	23.613269207053893	20.47971389813787	17.415217659390798	38.49179923541744
8	20.352989060052543	32.180959311288994	17.560650723368568	29.905400905289902
9	19.215352548685882	13.736880403547069	18.292818292818293	48.75494875494876
10-11	23.58654628875897	20.802807173339254	26.62621179604825	28.984434741853526
12-13	21.744492957399046	14.370112730950444	31.272971706258172	32.61242260539234
14-15	25.902812037493835	19.44499259990133	20.987913172175627	33.664282190429205
16-17	29.236072994591396	16.478670852117496	21.760849588958305	32.5244065643328
18-19	21.83233222738878	15.830630141717133	27.932089248492176	34.40494838240191
20-21	29.001344367838776	15.640918116898334	23.245229960902332	32.11250755436056
22-23	30.731042478661998	14.662168829246633	21.9651931521042	32.64159553998717
24-25	22.772008583902718	21.076194469796008	19.50926222836141	36.64253471793986
26-27	28.054586041463686	20.191655464154014	22.217974174611204	29.535784319771096
28-29	24.536260483473114	20.262703502713368	21.89257523433646	33.30846077947706
30-31	23.560500009250294	19.560672681413692	23.36624382542844	33.51258348390757
32-33	24.508975647658808	18.33424806211111	23.87380442893174	33.282971861298336
34-35	24.09580601994314	19.224721110501292	23.376767533500654	33.30270533605491
36-37	28.87502004267548	14.44922727777298	28.706661568632285	27.969091110919248
38-39	27.908109774899785	12.849213691026826	34.11717545482578	25.12550107924761
40-41	25.75220311179906	21.250393137514877	22.224757488113372	30.77264626257269
42-43	20.283661702586873	21.996731723861497	25.51475349181389	32.20485308173774
44-45	20.516916018106247	16.38134119417344	29.409080257039605	33.692662530680714
46-47	27.56351751356685	15.332387765170202	25.87876171682289	31.22533300444006
48-49	26.884154050137216	15.847183250593568	28.510375874934475	28.75828682433474
50-51	34.967747505519306	14.200347808927097	25.779178332243863	25.052726353309733
52-53	19.708789955041905	18.56047757954006	28.47037637757399	33.260356087844045
54-55	26.410405052049928	16.40312792935024	24.97903201933988	32.20743499925995
56-57	26.64298620489766	17.21273564834514	23.787147182703396	32.3571309640538
58-59	20.28552927754309	19.637383984459316	27.252320310813726	32.82476642718387
60-61	20.007153781636983	18.02506290394198	26.960506191721343	35.00727712269969
62-63	19.991489571641775	19.984705896863474	26.4088459119109	33.61495861958385
64-65	19.985322594570594	21.61155444824056	25.57075372793764	32.832369229251206
66-67	18.23693023083707	29.01467169085224	18.876465473114234	33.871932605196456
68-69	18.718810720784628	28.039971625080963	19.31776825093298	33.92344940320143
70-71	17.78421880299713	29.9904412444883	18.23872221023095	33.98661774228362
72-73	17.32765159690917	31.60578945096419	17.322101420228545	33.7444575318981
74-75	19.658725802771386	24.274006055859445	21.240526156749322	34.826741984619844
76	21.334040480037494	22.761078974308376	22.635272642056318	33.269607903597816
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.5
4	2.0
5	1.0
6	0.5
7	1.5
8	2.0
9	1.0
10	1.0
11	1.0
12	0.0
13	0.0
14	1.0
15	2.0
16	2.0
17	2.0
18	3.5
19	7.5
20	11.0
21	12.0
22	17.0
23	29.0
24	46.5
25	57.0
26	83.5
27	129.5
28	174.0
29	199.0
30	228.5
31	343.0
32	479.0
33	530.0
34	610.5
35	760.5
36	940.0
37	1050.0
38	1157.5
39	1391.5
40	1644.0
41	1894.5
42	2019.0
43	2138.5
44	2286.5
45	2436.5
46	2558.0
47	2626.0
48	2740.0
49	2767.5
50	2749.0
51	2906.5
52	3115.0
53	3177.0
54	3188.0
55	3288.0
56	3570.5
57	3760.0
58	3767.0
59	3743.5
60	3700.0
61	3683.0
62	3686.0
63	3653.0
64	3464.5
65	3124.0
66	2827.5
67	2716.0
68	2501.0
69	2091.0
70	1723.5
71	1551.0
72	1405.5
73	1123.0
74	886.5
75	787.0
76	697.0
77	541.0
78	405.0
79	335.0
80	285.0
81	184.5
82	118.0
83	102.0
84	77.5
85	42.0
86	20.0
87	9.0
88	7.5
89	3.5
90	2.5
91	3.0
92	2.0
93	1.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.003699593044765076
5	0.0
6	0.0
7	0.0
8	0.013565174497471945
9	0.011098779134295227
10-11	0.014798372179060304
12-13	0.014798372179060304
14-15	0.012331976815883588
16-17	0.01911456406461956
18-19	0.016031569860648662
20-21	0.013565174497471945
22-23	0.017264767542237023
24-25	0.009865581452706869
26-27	0.008632383771118511
28-29	0.012331976815883588
30-31	0.014181773338266123
32-33	0.011715377975089408
34-35	0.011715377975089408
36-37	0.016031569860648662
38-39	0.01849796522382538
40-41	0.014181773338266123
42-43	0.00924898261191269
44-45	0.016031569860648662
46-47	0.012331976815883588
48-49	0.015414971019854482
50-51	0.013565174497471945
52-53	0.0178813663830312
54-55	0.017264767542237023
56-57	0.012948575656677766
58-59	0.015414971019854482
60-61	0.017264767542237023
62-63	0.016031569860648662
64-65	0.016031569860648662
66-67	0.01911456406461956
68-69	0.040078924651621654
70-71	0.015414971019854482
72-73	0.014181773338266123
74-75	0.014181773338266123
76	0.016031569860648662
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	81090.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.58305586385498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.7340663739508	97.33505981008756
2	1.1145719968476753	2.1975582685904547
3	0.1338487134261518	0.3958564557898631
4	0.015011070664615154	0.059193488716241215
5	0.0025018451107691924	0.012331976815883588
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.003699593044765076	0.0
5	0.0	0.0	0.0	0.007399186089530152	0.0
6	0.0	0.0	0.0	0.007399186089530152	0.0
7	0.0	0.0	0.0	0.007399186089530152	0.0
8	0.0	0.0	0.0	0.007399186089530152	0.0
9	0.0	0.0	0.0	0.011098779134295227	0.0
10	0.0	0.0	0.0	0.014798372179060304	0.0
11	0.0	0.0	0.0	0.014798372179060304	0.0
12	0.0	0.0	0.0	0.014798372179060304	0.0
13	0.0	0.0	0.0	0.014798372179060304	0.0
14	0.0	0.0	0.0	0.01849796522382538	0.0
15	0.0	0.0	0.0	0.01849796522382538	0.0
16	0.0	0.0	0.0	0.019731162905413737	0.0
17	0.0	0.0	0.0	0.019731162905413737	0.0
18	0.0	0.0	0.0	0.028363546676532247	0.0
19	0.0	0.0	0.0	0.03452953508447404	0.0
20	0.0	0.0	0.0	0.039462325810827474	0.0
21	0.0	0.0	0.0	0.039462325810827474	0.0
22	0.0	0.0	0.0	0.05549389567147614	0.0
23	0.0	0.0	0.0	0.057960291034652854	0.0
24	0.0	0.0	0.0	0.06165988407941793	0.0
25	0.0	0.0	0.0	0.06535947712418301	0.0
26	0.0	0.0	0.0	0.07522505857688988	0.0
27	0.0	0.0	0.0	0.09495622148230361	0.0
28	0.0	0.0	0.0	0.12578616352201258	0.0
29	0.0	0.0	0.0	0.15291651251695645	0.0
30	0.0	0.0	0.0	0.19854482673572574	0.0
31	0.0	0.0	0.0	0.2145763965963744	0.0
32	0.0	0.0	0.0	0.27500308299420395	0.0
33	0.0	0.0	0.0	0.3378961647552103	0.0
34	0.0	0.0	0.0	0.41065482796892344	0.0
35	0.0	0.0	0.0	0.5734369219385868	0.0
36	0.0	0.0	0.0	0.7127882599580713	0.0
37	0.0	0.0	0.0	1.0272536687631026	0.0
38	0.0	0.0	0.0	1.2923911703045998	0.0
39	0.0	0.0	0.0	1.6191885559255148	0.0
40	0.0	0.0	0.0	1.9792822789493156	0.0
41	0.0	0.0	0.0	2.355407571833765	0.0
42	0.0	0.0	0.0	2.7315328647182144	0.0
43	0.0	0.0	0.0	3.036132692070539	0.0
44	0.0	0.0	0.0	3.434455543223579	0.0
45	0.0	0.0	0.0	3.6983598470834873	0.0
46	0.0	0.0	0.0	4.0202244419780495	0.0
47	0.0	0.0	0.0	4.381551362683438	0.0
48	0.0	0.0	0.0	4.625724503637933	0.0
49	0.0	0.0	0.0	4.860032063139721	0.0
50	0.0	0.0	0.0	5.130102355407572	0.0
51	0.0	0.0	0.0	5.46183253175484	0.0
52	0.0	0.0	0.0	5.778764335923048	0.0
53	0.0	0.0	0.0	6.1758539893945	0.0
54	0.0	0.0	0.0	6.527315328647182	0.0
55	0.0	0.0	0.0	6.788753237143914	0.0
56	0.0	0.0	0.0	7.098285855222592	0.0
57	0.0	0.0	0.0	7.370822542853619	0.0
58	0.0	0.0	0.0	7.613762486126526	0.0
59	0.0	0.0	0.0	7.8764335923048465	0.0
60	0.0	0.0	0.0	8.034282895548156	0.0
61	0.0	0.0	0.0	8.200764582562584	0.0
62	0.0	0.0	0.0	8.308052780860772	0.0
63	0.0	0.0	0.0	8.469601677148846	0.0
64	0.0	0.0	0.0	8.629917375755333	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCAT	15	0.0022173477	70.00062	57
AAATAAC	15	0.0022173477	70.00062	66
GCAGTGT	40	1.3278623E-10	70.00062	33
CAATTCC	15	0.0022173477	70.00062	70
GAGGTAT	15	0.0022173477	70.00062	54
GTAGTCT	15	0.0022173477	70.00062	38
TAGATCT	130	0.0	64.61595	39
CTGTGTA	45	3.783498E-10	62.222775	34
TGTAGAT	170	0.0	61.765247	37
TGTGTAG	70	0.0	60.00053	35
AAGTGTA	35	2.941124E-7	60.00053	34
GTGTAGA	200	0.0	59.500526	36
TCTGACA	30	8.309838E-6	58.333847	11
GTGTACT	30	8.309838E-6	58.333847	36
CGTGTAG	110	0.0	57.273235	35
CGTATAG	25	2.3776427E-4	56.000496	35
AAATTAT	25	2.3776427E-4	56.000496	56
GATTATT	25	2.3776427E-4	56.000496	57
AATAACA	25	2.3776427E-4	56.000496	67
CCTACGA	90	0.0	54.444927	21
>>END_MODULE