FastQCFastQC Report
Wed 25 May 2016
SRR1779773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81090
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2551631.466272043408562No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG30673.782217289431496No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC18862.325810827475644No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT8881.0950795412504626RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC8060.993957331360217No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC6580.81144407448514No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT4390.5413737822172894No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3870.4772475027746948No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC2470.30459982735232455No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2140.2639043038599087No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1990.24540633863608338No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1890.2330743618201998No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT1890.2330743618201998No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1680.20717721050684426No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCG1460.18004686151190036No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT1160.1430509310642496No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT1120.13811814033789618No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1040.1282525588851893No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT840.10358860525342213No Hit
TCTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT830.10235540757183377No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA207.884307E-570.000011
AACCCCC207.884307E-570.0000170
AAAAGAG207.884307E-570.0000168
TCTTAAT150.002217430270.000011
TCTTTAT401.3278623E-1070.000011
TTTACAC800.065.625012
CTTTACA800.065.625011
TTACACA850.061.764713
CTATACA1250.061.61
GTTATCT401.0506483E-861.25000433
AGTTATC352.941324E-760.00000432
ACAAACA252.377755E-456.00000470
TAAACTC252.377755E-456.00000434
GAGGTTG252.377755E-456.0000045
GTTAAAC252.377755E-456.00000432
TTAAACT252.377755E-456.00000433
TTATCTC452.6619091E-854.44444734
TTAATCT36700.053.40599434
TAATCTC36850.053.37856335
GTTAATC36700.053.31062733