FastQCFastQC Report
Wed 25 May 2016
SRR1779772_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779772_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173496
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAC200.006927777552.5274867
CCGCTTT200.00694360652.4971915
TAACGCG200.00694360652.4971923
CCGTCAA200.00694360652.4971921
TACGAGA200.00694360652.4971923
TATCGAG200.00694360652.497193
AGATCTC1500.051.33058540
GTGTATC352.080739E-549.99732236
TCGGTGG1450.048.27327345
GTAGATC1250.047.59745438
TTGGCAA305.8670744E-446.66416528
TGGCAAC305.8670744E-446.66416529
CTCGGTG1800.044.71982644
ACGCTTC1050.043.33101315
ATCTCGG1700.043.2329842
GCCGACT901.8189894E-1242.77548620
TGGTCGC1250.041.99775349
GACGCTT757.4942363E-1041.9977514
GCGTGTA503.465033E-641.9977534
CGACTAA503.465033E-641.9977522
Produced by FastQC (version 0.11.5)