##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779772_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	173496
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.136637155899848	34.0	23.0	34.0	12.0	34.0
2	27.782029556877394	34.0	23.0	34.0	12.0	34.0
3	26.743815419375665	32.0	21.0	34.0	11.0	34.0
4	28.84725872642597	34.0	23.0	34.0	12.0	34.0
5	28.55596670816618	34.0	23.0	34.0	11.0	34.0
6	30.810364504080784	36.0	26.0	38.0	11.0	38.0
7	31.833241112187025	37.0	28.0	38.0	12.0	38.0
8	31.04298658182321	37.0	27.0	38.0	11.0	38.0
9	32.46246022963066	37.0	31.0	38.0	12.0	38.0
10-11	32.10720708258404	37.0	31.0	38.0	11.0	38.0
12-13	31.248440886245213	36.0	27.5	38.0	11.0	38.0
14-15	31.138939802646746	36.5	26.5	38.0	11.0	38.0
16-17	30.883121224696822	36.0	27.0	38.0	11.0	38.0
18-19	31.459892447088116	37.0	27.5	38.0	11.5	38.0
20-21	30.328503250795407	35.5	25.0	38.0	11.0	38.0
22-23	30.343722621847192	35.5	26.0	38.0	11.0	38.0
24-25	31.446652948771153	36.5	28.5	38.0	11.0	38.0
26-27	30.08033326416747	35.5	24.0	38.0	11.0	38.0
28-29	32.035833679162636	37.0	31.0	38.0	11.0	38.0
30-31	32.4751636925347	37.5	31.0	38.0	11.0	38.0
32-33	32.562085004841606	38.0	31.5	38.0	11.0	38.0
34-35	32.619449439756536	38.0	32.0	38.0	11.0	38.0
36-37	30.707036473463365	36.5	25.0	38.0	11.0	38.0
38-39	29.11251556231844	34.0	23.0	37.5	11.0	38.0
40-41	30.18302151058238	35.0	25.0	38.0	11.0	38.0
42-43	31.14657686632545	36.5	28.0	38.0	11.0	38.0
44-45	32.68822624152718	37.5	32.0	38.0	11.0	38.0
46-47	31.337540923133673	37.0	28.5	38.0	11.0	38.0
48-49	30.79879939595149	36.0	26.0	38.0	11.0	38.0
50-51	28.750435168534146	34.0	21.5	37.5	11.0	38.0
52-53	31.85757308525845	36.5	29.0	38.0	11.0	38.0
54-55	30.989455088301746	36.5	27.0	38.0	11.0	38.0
56-57	31.379933831327524	36.5	28.0	38.0	11.0	38.0
58-59	32.39328572416655	37.0	31.5	38.0	11.0	38.0
60-61	32.8510484391571	38.0	34.0	38.0	11.0	38.0
62-63	32.76863443537603	38.0	33.0	38.0	11.0	38.0
64-65	32.527046733065895	37.5	32.5	38.0	11.0	38.0
66-67	30.54574457048001	36.5	25.0	38.0	11.0	38.0
68-69	30.65343293217135	36.5	25.0	38.0	11.0	38.0
70-71	30.29143034997925	35.5	24.0	38.0	11.0	38.0
72-73	29.18631265274127	34.0	22.5	37.5	11.0	38.0
74-75	31.11263660257297	36.5	27.0	38.0	11.0	38.0
76	31.354209895329	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	1.0
5	5.0
6	1.0
7	1.0
8	0.0
9	0.0
10	0.0
11	12.0
12	52.0
13	297.0
14	800.0
15	1497.0
16	2541.0
17	3857.0
18	5184.0
19	5520.0
20	4864.0
21	3970.0
22	3090.0
23	2645.0
24	2492.0
25	2453.0
26	2815.0
27	3178.0
28	3830.0
29	4447.0
30	5608.0
31	7062.0
32	9445.0
33	12804.0
34	17519.0
35	23414.0
36	28016.0
37	16074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.6568220592982	17.60674597685249	11.244639645870798	31.49179231797851
2	21.165905842209618	16.57848019550883	38.817033245723245	23.438580716558306
3	19.835615806704475	16.91624014386499	37.380688891962926	25.867455157467607
4	14.346728454834693	19.363558814036057	32.98692765251072	33.30278507861853
5	14.495435053257713	28.65483930465256	39.705814543274776	17.14391109881496
6	27.18103011020427	32.889519066722	17.296652372388987	22.632798450684742
7	27.27325125651312	26.36660211186425	20.16415364042975	26.195992991192877
8	23.77065716739583	32.28253413800458	17.732164369664584	26.21464432493501
9	23.034180644417546	16.436105827425212	19.56251080753934	40.9672027206179
10-11	24.357078833677743	23.708897861780954	27.08209043407086	24.851932870470442
12-13	24.159079577255735	19.640546328079292	29.107043528133474	27.0933305665315
14-15	23.68082034255873	22.584205869553315	23.65661124512576	30.0783625427622
16-17	25.012681430926552	22.72973564980805	26.042183050691136	26.215399868574263
18-19	22.12503134285772	22.66946038406889	27.84485069271318	27.360657580360204
20-21	24.341024502994426	22.204289609139487	27.199130780625865	26.25555510724023
22-23	25.200737815949502	21.654033489927084	27.39862235928178	25.746606334841626
24-25	22.475329705800977	24.82102508530849	24.182940606843125	28.520704602047402
26-27	23.379733702230677	24.048071934981845	27.3214594501124	25.25073491267508
28-29	23.173293907088944	25.049787161685067	26.128821218697496	25.648097712528497
30-31	22.769495032754712	24.334458300789976	26.430298611131125	26.46574805532419
32-33	22.744132828779186	24.30419658013736	26.468341917094413	26.483328673989043
34-35	23.302610570244457	24.237262734383556	26.465959985474413	25.99416670989757
36-37	24.301734747108515	21.601477950352628	30.428253983808258	23.6685333187306
38-39	23.87604584874066	20.79880795579983	33.39164819709079	21.93349799836872
40-41	23.288670104073297	23.134476765801935	25.911397913922652	27.66545521620212
42-43	20.747762131753234	23.806134035771308	27.53601051351367	27.910093318961792
44-45	20.881692600779896	21.944323556283127	29.788251976401348	27.38573186653563
46-47	23.120885835168256	21.252147147320244	29.601812249979826	26.02515476753167
48-49	23.145383489157627	21.9158894665852	30.4710466550615	24.46768038919567
50-51	25.115930542546295	20.359391887023563	31.56308091361049	22.961596656819655
52-53	19.60814830186069	23.225196559913304	29.84309792257499	27.32355721565101
54-55	21.73975698047082	22.391978510986604	29.117382582831848	26.75088192571073
56-57	21.809514751698238	23.05138380930148	28.05402087170043	27.08508056729985
58-59	19.56514847147728	23.93009173730218	29.061962307871887	27.442797483348656
60-61	19.484906302058416	23.169993601678552	28.801669327830393	28.543430768432643
62-63	19.412332656031587	23.57442393324975	28.53083164007897	28.482411770639686
64-65	19.869672105161527	24.206922192344603	27.580402973159142	28.343002729334728
66-67	19.002712120382405	25.04950124077346	23.79143477220783	32.15635186663631
68-69	19.437397661493048	25.427803786813037	23.88523096792048	31.249567583773434
70-71	19.289841914890552	25.7847333304512	23.06860922571441	31.856815528943844
72-73	18.787899748679994	25.62397685089115	21.893949874339995	33.69417352608886
74-75	20.38948836780337	25.76923520324641	23.958981808120633	29.882294620829587
76	21.45096954185977	25.096838901570173	24.559037144635816	28.89315441193424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	0.5
6	1.0
7	1.0
8	0.0
9	0.0
10	1.0
11	1.0
12	0.0
13	0.5
14	1.0
15	3.5
16	9.5
17	13.0
18	13.0
19	15.0
20	22.5
21	28.0
22	39.0
23	66.5
24	124.0
25	165.0
26	222.5
27	363.0
28	550.5
29	655.0
30	790.5
31	1089.0
32	1557.0
33	1862.0
34	2110.0
35	2858.5
36	3831.5
37	4304.0
38	4880.0
39	6081.5
40	7414.5
41	8608.0
42	9094.0
43	9470.0
44	10205.5
45	10755.5
46	10946.0
47	10753.5
48	10377.0
49	9851.5
50	9510.0
51	8917.0
52	7840.0
53	6835.5
54	6315.0
55	5828.0
56	4919.5
57	4144.0
58	3790.0
59	3598.0
60	3215.5
61	2897.0
62	2769.0
63	2632.0
64	2461.0
65	2300.0
66	2100.5
67	2028.0
68	2019.5
69	1879.5
70	1722.0
71	1696.0
72	1593.5
73	1413.5
74	1264.5
75	1193.0
76	1058.5
77	833.5
78	654.0
79	565.0
80	470.5
81	307.0
82	202.5
83	167.0
84	126.0
85	76.5
86	51.0
87	34.0
88	25.0
89	13.5
90	7.5
91	5.0
92	6.0
93	4.5
94	2.0
95	1.5
96	2.0
97	1.5
98	0.5
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.005187439479872735
9	0.003458292986581823
10-11	0.00605201272651819
12-13	0.00605201272651819
14-15	0.0043228662332272785
16-17	0.008069350302024254
18-19	0.006628394890948494
20-21	0.004034675151012127
22-23	0.006340203808733343
24-25	0.004611057315442431
26-27	0.003458292986581823
28-29	0.0043228662332272785
30-31	0.005475630562087887
32-33	0.005475630562087887
34-35	0.005187439479872735
36-37	0.007204777055378798
38-39	0.007204777055378798
40-41	0.007204777055378798
42-43	0.0028819108221515193
44-45	0.006628394890948494
46-47	0.005763821644303039
48-49	0.005763821644303039
50-51	0.004899248397657583
52-53	0.006916585973163646
54-55	0.006916585973163646
56-57	0.0043228662332272785
58-59	0.006628394890948494
60-61	0.00749296813759395
62-63	0.0077811592198091025
64-65	0.00605201272651819
66-67	0.00893392354866971
68-69	0.0299718725503758
70-71	0.0077811592198091025
72-73	0.006916585973163646
74-75	0.006916585973163646
76	0.006916585973163646
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	173496.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.84523677611132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.33389778136123	83.85606145403771
2	4.532232736841612	8.143990467319554
3	1.0717441849442904	2.8887333017922066
4	0.49356221936590167	1.7737685785068975
5	0.2434973996363353	1.0938540762346973
6	0.13146653180290616	0.7086985006779764
7	0.08445195179885145	0.5311323923900785
8	0.03672607139158833	0.2639730064026898
9	0.02182875304452796	0.1765088537231588
>10	0.05059236981276668	0.5632793689150385
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.763821644303039E-4	0.0
2	0.0	0.0	0.0	5.763821644303039E-4	0.0
3	0.0	0.0	0.0	5.763821644303039E-4	0.0
4	0.0	0.0	0.0	0.0011527643288606077	0.0
5	0.0	0.0	0.0	0.0017291464932909116	0.0
6	0.0	5.763821644303039E-4	0.0	0.0017291464932909116	0.0
7	0.0	5.763821644303039E-4	0.0	0.0028819108221515193	0.0
8	0.0	5.763821644303039E-4	0.0	0.0028819108221515193	0.0
9	0.0	5.763821644303039E-4	0.0	0.004034675151012127	0.0
10	0.0	5.763821644303039E-4	0.0	0.004034675151012127	0.0
11	0.0	5.763821644303039E-4	0.0	0.005187439479872735	0.0
12	0.0	5.763821644303039E-4	0.0	0.005187439479872735	5.763821644303039E-4
13	0.0	5.763821644303039E-4	0.0	0.005187439479872735	5.763821644303039E-4
14	0.0	5.763821644303039E-4	0.0	0.005763821644303039	5.763821644303039E-4
15	0.0	5.763821644303039E-4	0.0	0.005763821644303039	5.763821644303039E-4
16	0.0	5.763821644303039E-4	0.0	0.005763821644303039	5.763821644303039E-4
17	0.0	5.763821644303039E-4	0.0	0.005763821644303039	5.763821644303039E-4
18	0.0	5.763821644303039E-4	0.0	0.006916585973163646	5.763821644303039E-4
19	0.0	5.763821644303039E-4	0.0	0.009798496795315166	5.763821644303039E-4
20	0.0	5.763821644303039E-4	0.0	0.010951261124175774	5.763821644303039E-4
21	0.0	5.763821644303039E-4	0.0	0.01325678978189699	5.763821644303039E-4
22	0.0	5.763821644303039E-4	0.0	0.01786784709733942	5.763821644303039E-4
23	0.0	5.763821644303039E-4	0.0	0.02247890441278185	5.763821644303039E-4
24	0.0	5.763821644303039E-4	0.0	0.02536081523493337	5.763821644303039E-4
25	0.0	5.763821644303039E-4	0.0	0.028819108221515195	5.763821644303039E-4
26	0.0	5.763821644303039E-4	0.0	0.032277401208097016	5.763821644303039E-4
27	0.0	5.763821644303039E-4	0.0	0.06570756674505464	5.763821644303039E-4
28	0.0	5.763821644303039E-4	0.0	0.104325171761885	5.763821644303039E-4
29	0.0	5.763821644303039E-4	0.0	0.12968598699681838	5.763821644303039E-4
30	0.0	5.763821644303039E-4	0.0	0.16945635634250933	5.763821644303039E-4
31	0.0	5.763821644303039E-4	0.0	0.18559505694655784	5.763821644303039E-4
32	0.0	5.763821644303039E-4	0.0	0.23343477659427306	5.763821644303039E-4
33	0.0	5.763821644303039E-4	0.0	0.2824272605708489	5.763821644303039E-4
34	0.0	0.0011527643288606077	0.0	0.3435237700004611	5.763821644303039E-4
35	0.0	0.0011527643288606077	0.0	0.46514040669525525	5.763821644303039E-4
36	0.0	0.0011527643288606077	0.0	0.5556324065108129	5.763821644303039E-4
37	0.0	0.0011527643288606077	0.0	0.8737953612763406	5.763821644303039E-4
38	0.0	0.0011527643288606077	0.0	1.1902291695485774	5.763821644303039E-4
39	0.0	0.0011527643288606077	0.0	1.578134366210172	5.763821644303039E-4
40	0.0	0.0011527643288606077	0.0	2.0813159957578273	5.763821644303039E-4
41	0.0	0.0011527643288606077	0.0	2.6888227970673677	5.763821644303039E-4
42	0.0	0.0011527643288606077	0.0	3.302093420021211	5.763821644303039E-4
43	0.0	0.0011527643288606077	0.0	3.928044450592521	5.763821644303039E-4
44	0.0	0.0011527643288606077	0.0	4.651404066952552	5.763821644303039E-4
45	0.0	0.0011527643288606077	0.0	5.100405773043759	0.0011527643288606077
46	0.0	0.0011527643288606077	0.0	5.675635173145202	0.0011527643288606077
47	0.0	0.0011527643288606077	0.0	6.388619910545488	0.0011527643288606077
48	0.0	0.0011527643288606077	0.0	6.868746253515932	0.0011527643288606077
49	0.0	0.0011527643288606077	0.0	7.296998201687647	0.0011527643288606077
50	0.0	0.0011527643288606077	0.0	7.978858302208696	0.0011527643288606077
51	0.0	0.0011527643288606077	0.0	8.713169179692903	0.0011527643288606077
52	0.0	0.0011527643288606077	5.763821644303039E-4	9.377737815281044	0.0011527643288606077
53	0.0	0.0011527643288606077	5.763821644303039E-4	10.230207036473463	0.0011527643288606077
54	0.0	0.0011527643288606077	5.763821644303039E-4	10.995642550836907	0.0011527643288606077
55	0.0	0.0011527643288606077	5.763821644303039E-4	11.468275925669756	0.0011527643288606077
56	0.0	0.0011527643288606077	5.763821644303039E-4	11.996241988287915	0.0011527643288606077
57	0.0	0.0011527643288606077	5.763821644303039E-4	12.47982662424494	0.0011527643288606077
58	0.0	0.0011527643288606077	5.763821644303039E-4	12.9184534513764	0.0011527643288606077
59	0.0	0.0011527643288606077	5.763821644303039E-4	13.40953105547102	0.0011527643288606077
60	0.0	0.0011527643288606077	5.763821644303039E-4	13.717895513441231	0.0011527643288606077
61	0.0	0.0011527643288606077	5.763821644303039E-4	14.051620786646378	0.0011527643288606077
62	0.0	0.0011527643288606077	5.763821644303039E-4	14.316180200119888	0.0011527643288606077
63	0.0	0.0011527643288606077	5.763821644303039E-4	14.647023562502882	0.0011527643288606077
64	0.0	0.0011527643288606077	5.763821644303039E-4	14.974408631899294	0.0011527643288606077
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAGAC	20	0.0069277775	52.52748	67
CCGCTTT	20	0.006943606	52.49719	15
TAACGCG	20	0.006943606	52.49719	23
CCGTCAA	20	0.006943606	52.49719	21
TACGAGA	20	0.006943606	52.49719	23
TATCGAG	20	0.006943606	52.49719	3
AGATCTC	150	0.0	51.330585	40
GTGTATC	35	2.080739E-5	49.997322	36
TCGGTGG	145	0.0	48.273273	45
GTAGATC	125	0.0	47.597454	38
TTGGCAA	30	5.8670744E-4	46.664165	28
TGGCAAC	30	5.8670744E-4	46.664165	29
CTCGGTG	180	0.0	44.719826	44
ACGCTTC	105	0.0	43.331013	15
ATCTCGG	170	0.0	43.23298	42
GCCGACT	90	1.8189894E-12	42.775486	20
TGGTCGC	125	0.0	41.997753	49
GACGCTT	75	7.4942363E-10	41.99775	14
GCGTGTA	50	3.465033E-6	41.99775	34
CGACTAA	50	3.465033E-6	41.99775	22
>>END_MODULE