FastQCFastQC Report
Wed 25 May 2016
SRR1779772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173496
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2117212.203163185318394No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG25791.4864896020657536No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC16460.9487250426522802No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT9280.534882648591322Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC6070.34986397380919443No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC5500.3170101904366671No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT4340.25014985936275186No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC3960.22824733711440032No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2220.12795684050352746No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG2200.12680407617466685No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC1830.10547793609074561No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAT150.002221712470.025
ATGCGAG150.002221712470.013
ATAGTTC150.002221712470.041
CTTGTAC308.350256E-658.3333323
GGGGACT200.0069421452.516
TCCGCTG200.0069421452.570
TACTTGG200.0069421452.57
ATGTCCG200.0069421452.534
ATCTTGC200.0069421452.543
GCCACTC200.0069421452.533
GTCGGGT200.0069421452.54
TGGTACC200.0069421452.515
CTTTATA750.051.3333362
CCGTCTT32500.051.26153647
TCTCGTA31350.051.2440238
CTTGAAA32050.051.2168557
TCTTCTG32400.051.203750
CGTCTTC32500.051.15384348
ATCTCGT31500.051.037
TATGCCG32750.050.977143
Produced by FastQC (version 0.11.5)