FastQCFastQC Report
Wed 25 May 2016
SRR1779771_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779771_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169086
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGT150.00222213669.9958633
GAGAGAC150.00222213669.9958660
ATCTCGG1400.054.99674642
TAGATCT1550.054.19034639
TCGGTGG1100.054.0877145
GCCGTAT653.6379788E-1253.84296854
AGCAAGG200.006943458752.4968955
CTTCCGA800.052.49689518
ACGCATT200.006943458752.49689542
GCAACCG200.006943458752.49689528
TCCGGTG200.006943458752.49689544
AGATCTC1700.051.4675440
GTAGATC1300.048.4586738
TTCCGAC950.047.89190719
ACACTCG305.849722E-446.69152568
TCTCGGT1650.046.6639143
CAGTGTA451.677894E-646.66390634
TTCGGTG305.8668177E-446.66390244
CGCGTAT305.8668177E-446.66390235
TTAGATC551.3043427E-744.5428238
Produced by FastQC (version 0.11.5)