##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779771_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	169086
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.247554498894054	34.0	23.0	34.0	12.0	34.0
2	27.801107128916644	34.0	23.0	34.0	12.0	34.0
3	26.810132122115373	32.0	21.0	34.0	11.0	34.0
4	28.95949398530925	34.0	23.0	34.0	12.0	34.0
5	28.500839809327797	34.0	23.0	34.0	11.0	34.0
6	30.936381486344228	36.0	26.0	38.0	11.0	38.0
7	31.9274925186	37.0	28.0	38.0	12.0	38.0
8	31.21267284103947	37.0	27.0	38.0	11.0	38.0
9	32.55655701832204	37.0	31.0	38.0	12.0	38.0
10-11	32.25463965082857	37.0	31.0	38.0	11.0	38.0
12-13	31.337671362501922	36.0	28.0	38.0	11.0	38.0
14-15	31.28994712749725	36.5	27.0	38.0	11.0	38.0
16-17	30.993387980081142	36.0	27.0	38.0	11.0	38.0
18-19	31.586577244715706	37.0	27.5	38.0	11.5	38.0
20-21	30.49611144624629	35.5	25.0	38.0	11.0	38.0
22-23	30.4916344345481	35.5	26.0	38.0	11.0	38.0
24-25	31.576777497841334	36.5	28.5	38.0	11.0	38.0
26-27	30.215177483647373	35.5	24.5	38.0	11.0	38.0
28-29	32.234380729333	37.0	31.0	38.0	11.0	38.0
30-31	32.66804466366228	37.5	32.5	38.0	11.0	38.0
32-33	32.74941745620572	38.0	33.0	38.0	11.0	38.0
34-35	32.77318346876737	38.0	33.5	38.0	11.0	38.0
36-37	30.866133210319006	36.5	25.0	38.0	11.0	38.0
38-39	29.224684480087056	34.0	23.0	37.5	11.0	38.0
40-41	30.30712181966573	35.0	25.0	38.0	11.0	38.0
42-43	31.288013200383237	36.5	28.0	38.0	11.0	38.0
44-45	32.88272240161811	37.5	33.5	38.0	11.0	38.0
46-47	31.517097808215937	37.0	28.5	38.0	11.0	38.0
48-49	30.940536176856746	36.0	27.0	38.0	11.0	38.0
50-51	28.83557183918243	34.0	21.5	37.5	11.0	38.0
52-53	32.038211324414796	37.0	29.0	38.0	11.0	38.0
54-55	31.148025265249636	36.5	27.0	38.0	11.0	38.0
56-57	31.578563571200455	37.0	28.5	38.0	11.0	38.0
58-59	32.599490791668146	37.0	32.0	38.0	11.0	38.0
60-61	33.06440509563181	38.0	34.0	38.0	11.0	38.0
62-63	32.979471984670525	38.0	34.0	38.0	11.0	38.0
64-65	32.694764794246716	37.5	32.5	38.0	11.0	38.0
66-67	30.705910010290623	36.5	25.0	38.0	11.0	38.0
68-69	30.791138828761696	36.5	26.0	38.0	11.0	38.0
70-71	30.443437067527768	36.0	25.0	38.0	11.0	38.0
72-73	29.289950084572347	34.0	22.5	38.0	11.0	38.0
74-75	31.267390558650625	36.5	28.0	38.0	11.0	38.0
76	31.561406621482558	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	2.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	1.0
11	10.0
12	55.0
13	305.0
14	764.0
15	1382.0
16	2407.0
17	3634.0
18	4884.0
19	5151.0
20	4370.0
21	3563.0
22	2844.0
23	2379.0
24	2356.0
25	2334.0
26	2616.0
27	3017.0
28	3803.0
29	4405.0
30	5637.0
31	7232.0
32	9297.0
33	12869.0
34	17337.0
35	23291.0
36	27443.0
37	15693.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.42186816176383	18.61242208107117	11.661521355996356	31.304188401168638
2	20.135315756478953	16.50875885643992	38.94881894420591	24.40710644287522
3	20.61022201719835	17.837668405426825	35.570656352388724	25.9814532249861
4	15.125439125652035	18.87382751972369	33.50188661391245	32.498846740711826
5	14.048472375004437	29.483221555894634	38.77790000354849	17.69040606555244
6	26.335710821712027	33.844315910246856	17.36217072968785	22.457802538353263
7	26.827768117999124	26.617815786049704	20.81366878393244	25.740747312018737
8	22.898036432458007	32.13804116394606	18.47054648687012	26.49337591672581
9	23.220369056068133	16.534776437189496	20.303406671398154	39.94144783534422
10-11	24.751889660393427	23.826872804267854	26.842640674718176	24.578596860620543
12-13	24.114905546552478	19.727285631483692	28.81185015200085	27.34595866996298
14-15	23.81122768834057	22.42166522752274	23.81655054943755	29.950556534699142
16-17	24.84282123318054	22.738429690965546	26.29690965547834	26.121839420375576
18-19	22.469939731601578	22.36377508472471	28.038999982256605	27.127285201417106
20-21	24.21368582919328	22.30748757984386	27.57718239886444	25.901644192098416
22-23	25.32817596560178	21.304601117232526	27.502905455158405	25.864317462007293
24-25	22.791349631386417	24.65674431258668	24.16644241318433	28.385463642842566
26-27	23.311903762102187	23.945919411406365	27.425612576220864	25.31656425027058
28-29	23.185997202515964	24.619929560179916	26.624595976472747	25.56947726083138
30-31	23.139616035202685	23.99188540200381	26.597487550124793	26.27101101266871
32-33	22.84116549413438	24.342243395819104	26.618996501624988	26.197594608421532
34-35	23.33451620534658	24.366276318902294	26.45581973030518	25.843387745445945
36-37	24.261359435761705	21.645103576762136	30.388727063033222	23.704809924442934
38-39	23.923940204935608	20.950451715929084	33.25279827298132	21.872809806153985
40-41	23.351579296007476	22.792371590710825	26.2404740993089	27.615575013972798
42-43	21.08173645611545	23.440974686538915	27.421339011118995	28.055949846226643
44-45	20.677907960184292	22.24877128442917	29.719835106666114	27.353485648720422
46-47	22.97653759485211	21.47812560992199	30.085344720515263	25.459992074710634
48-49	22.989321528165913	21.85257143871042	30.451535825075332	24.706571208048334
50-51	24.516646064857255	20.660164775045985	31.67276835088923	23.15042080920753
52-53	19.55878221525632	23.499282873238602	29.83809199923112	27.103842912273958
54-55	21.41839021995635	22.771308086752345	29.276255478208412	26.534046215082892
56-57	21.519039747339285	22.936411945789136	28.56124745313623	26.983300853735354
58-59	19.686771785803003	24.137676102154035	28.76335182578455	27.412200286258415
60-61	19.489345091289767	23.150694653915078	28.665637549755434	28.694322705039717
62-63	19.49886589798109	23.99623838913155	28.262058890502228	28.24283682238513
64-65	20.181218136008233	24.088290611433777	27.254580726055426	28.475910526502563
66-67	19.00707667830834	24.996673103595118	23.71559619462196	32.28065402347458
68-69	19.704024042500325	25.523563306790347	23.77805648567744	30.99435616503189
70-71	19.541565750212182	25.6672315030859	22.895097160735883	31.896105585966033
72-73	18.901742992837583	25.47093927618777	21.895645179415297	33.73167255155935
74-75	20.552409398057105	25.798524345344592	24.172642723011638	29.476423533586672
76	21.434021185613656	25.018778426397443	24.634338200938032	28.91286218705087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.5
6	2.0
7	1.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.5
14	0.5
15	1.5
16	3.0
17	3.0
18	4.0
19	10.0
20	19.5
21	24.0
22	31.5
23	54.5
24	94.5
25	119.0
26	167.5
27	299.0
28	457.5
29	533.0
30	690.0
31	1005.0
32	1446.0
33	1729.0
34	2053.0
35	2821.5
36	3717.5
37	4169.0
38	4760.0
39	5989.0
40	7357.0
41	8586.5
42	9086.0
43	9487.0
44	10208.0
45	10730.5
46	10933.0
47	10887.0
48	10422.5
49	9732.0
50	9460.0
51	8749.5
52	7509.0
53	6399.0
54	5819.0
55	5432.5
56	4653.5
57	3891.0
58	3521.0
59	3327.0
60	2964.5
61	2706.0
62	2616.0
63	2477.5
64	2299.0
65	2194.5
66	2111.0
67	2092.0
68	2002.5
69	1845.0
70	1691.5
71	1606.0
72	1548.5
73	1382.5
74	1204.0
75	1134.0
76	1040.0
77	814.0
78	591.0
79	500.0
80	429.5
81	304.0
82	193.5
83	138.0
84	116.0
85	79.5
86	47.0
87	29.0
88	20.0
89	10.0
90	5.0
91	0.5
92	0.0
93	1.5
94	2.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0035484901174550228
9	0.0035484901174550228
10-11	0.004731320156606697
12-13	0.004731320156606697
14-15	0.0023656600783033485
16-17	0.006505565215334209
18-19	0.005322735176182535
20-21	0.0035484901174550228
22-23	0.0044356126468187785
24-25	0.0038441976272429413
26-27	0.0029570750978791856
28-29	0.0032527826076671046
30-31	0.004731320156606697
32-33	0.0044356126468187785
34-35	0.0035484901174550228
36-37	0.005027027666394616
38-39	0.005027027666394616
40-41	0.0044356126468187785
42-43	0.0035484901174550228
44-45	0.00413990513703086
46-47	0.00413990513703086
48-49	0.0044356126468187785
50-51	0.00413990513703086
52-53	0.005027027666394616
54-55	0.00413990513703086
56-57	0.0032527826076671046
58-59	0.004731320156606697
60-61	0.005322735176182535
62-63	0.005618442685970453
64-65	0.004731320156606697
66-67	0.005618442685970453
68-69	0.03075358101794353
70-71	0.006209857705546291
72-73	0.005322735176182535
74-75	0.005027027666394616
76	0.005322735176182535
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	169086.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.55769125158297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.77348591955632	82.15805722397147
2	4.706031241701844	8.335105234458709
3	1.2942971830231016	3.438599109658602
4	0.548867225045502	1.944256570147707
5	0.24100201248240566	1.0671290906213513
6	0.1592537530446156	0.8461886815668543
7	0.10631445286984889	0.6590473744980331
8	0.05560465310156118	0.3939375761215545
9	0.04268590136869123	0.3402148386783672
>10	0.0724576578061191	0.8174643002773414
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.914150195758371E-4	0.0
3	0.0	0.0	0.0	0.0029570750978791856	0.0
4	0.0	0.0	0.0	0.00413990513703086	0.0
5	0.0	0.0	0.0	0.004731320156606697	0.0
6	0.0	0.0	0.0	0.005322735176182534	0.0
7	0.0	0.0	0.0	0.005322735176182534	0.0
8	0.0	0.0	0.0	0.005322735176182534	0.0
9	0.0	0.0	0.0	0.005322735176182534	0.0
10	0.0	0.0	0.0	0.005322735176182534	0.0
11	0.0	0.0	0.0	0.00827981027406172	0.0
12	0.0	0.0	0.0	0.008871225293637557	0.0
13	0.0	0.0	0.0	0.008871225293637557	0.0
14	0.0	0.0	0.0	0.010645470352365068	0.0
15	0.0	0.0	0.0	0.011236885371940905	0.0
16	0.0	0.0	0.0	0.011828300391516743	0.0
17	0.0	0.0	0.0	0.013011130430668417	0.0
18	0.0	0.0	0.0	0.014193960469820091	0.0
19	0.0	0.0	0.0	0.017742450587275114	0.0
20	0.0	0.0	0.0	0.018925280626426788	0.0
21	0.0	0.0	0.0	0.021290940704730137	0.0
22	0.0	0.0	0.0	0.025430845841760998	5.914150195758371E-4
23	0.0	0.0	0.0	0.026022260861336834	5.914150195758371E-4
24	0.0	0.0	0.0	0.03252782607667104	5.914150195758371E-4
25	0.0	0.0	0.0	0.03430207113539856	5.914150195758371E-4
26	0.0	0.0	0.0	0.03607631619412607	5.914150195758371E-4
27	0.0	0.0	0.0	0.06742131223164544	5.914150195758371E-4
28	0.0	0.0	0.0	0.0969920632104373	5.914150195758371E-4
29	0.0	0.0	0.0	0.12478856913050164	5.914150195758371E-4
30	0.0	0.0	0.0	0.16441337544208273	5.914150195758371E-4
31	0.0	0.0	0.0	0.1845214861076612	5.914150195758371E-4
32	0.0	0.0	0.0	0.232426102693304	5.914150195758371E-4
33	0.0	0.0	0.0	0.28920194457258436	5.914150195758371E-4
34	0.0	0.0	0.0	0.3572146718238056	5.914150195758371E-4
35	0.0	0.0	0.0	0.49856286150243073	5.914150195758371E-4
36	0.0	0.0	0.0	0.6144802053392948	5.914150195758371E-4
37	0.0	0.0	0.0	0.9166932803425476	5.914150195758371E-4
38	0.0	0.0	0.0	1.2537998415007747	5.914150195758371E-4
39	0.0	0.0	0.0	1.6831671457128325	5.914150195758371E-4
40	0.0	0.0	0.0	2.167536046745443	5.914150195758371E-4
41	0.0	0.0	0.0	2.7554025762038252	5.914150195758371E-4
42	0.0	0.0	0.0	3.3077842044876573	5.914150195758371E-4
43	0.0	0.0	0.0	4.0056539275871454	5.914150195758371E-4
44	0.0	0.0	0.0	4.637285168494139	5.914150195758371E-4
45	0.0	0.0	0.0	5.0861691683522	5.914150195758371E-4
46	0.0	0.0	0.0	5.681724093065068	5.914150195758371E-4
47	0.0	0.0	0.0	6.426907017730622	5.914150195758371E-4
48	0.0	0.0	0.0	6.894716298215109	5.914150195758371E-4
49	0.0	0.0	0.0	7.349514448268928	5.914150195758371E-4
50	0.0	0.0	0.0	8.08227765752339	5.914150195758371E-4
51	0.0	0.0	0.0	8.78842719089694	5.914150195758371E-4
52	0.0	0.0	0.0	9.396993246040477	5.914150195758371E-4
53	0.0	0.0	0.0	10.15223022603882	5.914150195758371E-4
54	0.0	0.0	0.0	10.861336834510249	5.914150195758371E-4
55	0.0	0.0	0.0	11.314360739505341	5.914150195758371E-4
56	0.0	0.0	0.0	11.837763031829956	5.914150195758371E-4
57	0.0	0.0	0.0	12.259441940787529	5.914150195758371E-4
58	0.0	0.0	0.0	12.669292549353584	5.914150195758371E-4
59	0.0	0.0	0.0	13.182049371325833	5.914150195758371E-4
60	0.0	0.0	0.0	13.473616975976721	5.914150195758371E-4
61	0.0	0.0	0.0	13.787658351371492	5.914150195758371E-4
62	0.0	0.0	0.0	14.021267284103947	5.914150195758371E-4
63	0.0	0.0	0.0	14.312834888754836	5.914150195758371E-4
64	0.0	0.0	0.0	14.629241924227907	5.914150195758371E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAGTGT	15	0.002222136	69.99586	33
GAGAGAC	15	0.002222136	69.99586	60
ATCTCGG	140	0.0	54.996746	42
TAGATCT	155	0.0	54.190346	39
TCGGTGG	110	0.0	54.08771	45
GCCGTAT	65	3.6379788E-12	53.842968	54
AGCAAGG	20	0.0069434587	52.496895	5
CTTCCGA	80	0.0	52.496895	18
ACGCATT	20	0.0069434587	52.496895	42
GCAACCG	20	0.0069434587	52.496895	28
TCCGGTG	20	0.0069434587	52.496895	44
AGATCTC	170	0.0	51.46754	40
GTAGATC	130	0.0	48.45867	38
TTCCGAC	95	0.0	47.891907	19
ACACTCG	30	5.849722E-4	46.691525	68
TCTCGGT	165	0.0	46.66391	43
CAGTGTA	45	1.677894E-6	46.663906	34
TTCGGTG	30	5.8668177E-4	46.663902	44
CGCGTAT	30	5.8668177E-4	46.663902	35
TTAGATC	55	1.3043427E-7	44.54282	38
>>END_MODULE