FastQCFastQC Report
Wed 25 May 2016
SRR1779771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169086
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT1934511.440923553694569No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG22811.3490176596524845No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC14040.8303466874844753No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT8800.5204452172267368RNA PCR Primer, Index 30 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC5800.34302071135398554No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC5130.30339590504240443No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT5100.30162165998367696No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC3090.1827472410489337No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT2160.12774564422838083No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2030.12005724897389494No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1850.10941177862152987No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT1810.10704611854322653No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG1730.10231479838661982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCAA150.002221614470.010
CGTCCGA150.002221614470.014
CATATTC200.00694183752.53
ATCGTAT200.00694183752.539
TGCTATT200.00694183752.523
TATGTGG200.00694183752.538
GTTGCAT200.00694183752.544
CTTGAAA29150.050.78902457
TTGAAAA29200.050.70205358
TCTCGTA27900.050.68100438
CTGCTTG29200.050.5821954
CCGTCTT29350.050.5621847
CGTCTTC29550.050.4568548
TCGTATG29800.050.38590240
TGAAAAA29550.050.33840659
CGTATGC29650.050.28667441
ATCTCGT28050.050.28520237
GGATCTC28000.050.25000435
GCTTGAA29550.050.21996356
TTCTGCT29550.050.21996352
Produced by FastQC (version 0.11.5)