FastQCFastQC Report
Wed 25 May 2016
SRR1779770_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779770_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215928
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATA150.002222654969.9983821
GATCTAG252.3869486E-455.998741
ATCTCGG1750.049.99884442
TCTCGGT1950.048.4604243
GCGTGTA651.8371793E-1048.4604234
GATCTCG1700.047.3518441
GCGAGAA305.86941E-446.66558513
CGTGCGA305.86941E-446.66558510
CCGCGTA305.86941E-446.66558534
CTCGGTG2000.045.49894344
TGCCTAC851.8189894E-1245.29306819
GCCGTAT1250.044.7989654
ATTTCGG551.3062345E-744.54442242
GTGTCGA950.044.209536
CGCGTAG404.582983E-543.7489935
GCGTAGA404.582983E-543.7489936
TAGATCT1800.042.77678339
AGATCTC1400.042.4990240
CGGTGTT757.512426E-1041.99902746
CGTATCA1350.041.48052256
Produced by FastQC (version 0.11.5)