##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779770_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	215928
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.391968619169354	34.0	27.0	34.0	12.0	34.0
2	27.885434033566746	34.0	23.0	34.0	12.0	34.0
3	26.798858878885554	32.0	21.0	34.0	11.0	34.0
4	29.090798784780112	34.0	23.0	34.0	12.0	34.0
5	28.720740246748917	34.0	23.0	34.0	11.0	34.0
6	31.08496350635397	36.0	27.0	38.0	11.0	38.0
7	32.02961635359935	37.0	28.0	38.0	12.0	38.0
8	31.25705790819162	37.0	27.0	38.0	11.0	38.0
9	32.659641176688524	37.0	31.0	38.0	12.0	38.0
10-11	32.382615964580786	37.0	31.0	38.0	11.0	38.0
12-13	31.4328711422326	36.5	28.5	38.0	11.0	38.0
14-15	31.44302498981142	36.5	27.5	38.0	11.0	38.0
16-17	31.11788651772813	36.0	27.0	38.0	11.0	38.0
18-19	31.706724000592793	37.0	29.0	38.0	11.5	38.0
20-21	30.565366696306178	35.5	26.5	38.0	11.0	38.0
22-23	30.560619743618243	35.5	26.0	38.0	11.0	38.0
24-25	31.716921844318477	36.5	29.0	38.0	11.0	38.0
26-27	30.316061372309285	35.5	24.5	38.0	11.0	38.0
28-29	32.355912619021154	37.0	31.0	38.0	11.0	38.0
30-31	32.82737764439999	37.5	33.0	38.0	11.0	38.0
32-33	32.92596374717499	38.0	34.0	38.0	11.0	38.0
34-35	32.968964191767625	38.0	34.0	38.0	11.0	38.0
36-37	30.94728103812382	36.5	27.0	38.0	11.0	38.0
38-39	29.254297728872587	34.0	23.0	37.5	11.0	38.0
40-41	30.425000463117335	35.0	25.0	38.0	11.0	38.0
42-43	31.404861805787114	36.5	28.0	38.0	11.0	38.0
44-45	33.013212737579195	37.5	34.0	38.0	11.0	38.0
46-47	31.605868622874294	37.0	28.5	38.0	11.0	38.0
48-49	31.08378255714868	36.5	27.5	38.0	11.0	38.0
50-51	28.932512226297654	34.0	22.0	37.5	11.0	38.0
52-53	32.19675771553481	37.0	29.5	38.0	11.0	38.0
54-55	31.27594383312956	36.5	27.5	38.0	11.0	38.0
56-57	31.69139713237746	37.0	29.0	38.0	11.0	38.0
58-59	32.74666787077174	37.0	33.0	38.0	11.0	38.0
60-61	33.19891352673113	38.0	34.0	38.0	11.0	38.0
62-63	33.096860990700605	38.0	34.0	38.0	11.0	38.0
64-65	32.84183385202475	38.0	32.5	38.0	11.0	38.0
66-67	30.791266070171538	36.5	25.0	38.0	11.0	38.0
68-69	30.872144881627207	36.5	26.0	38.0	11.0	38.0
70-71	30.505228594716755	36.0	25.0	38.0	11.0	38.0
72-73	29.303490052239635	34.0	22.5	38.0	11.0	38.0
74-75	31.39112806120559	36.5	28.0	38.0	11.0	38.0
76	31.688914823459672	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	1.0
5	1.0
6	3.0
7	0.0
8	0.0
9	0.0
10	3.0
11	18.0
12	62.0
13	332.0
14	898.0
15	1656.0
16	2824.0
17	4400.0
18	5657.0
19	6147.0
20	5340.0
21	4330.0
22	3493.0
23	3134.0
24	3006.0
25	3159.0
26	3565.0
27	4087.0
28	4953.0
29	6117.0
30	7465.0
31	9547.0
32	12280.0
33	16456.0
34	22430.0
35	30055.0
36	34590.0
37	19917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.54731206698529	18.691415657070877	10.917991182245935	29.8432810936979
2	21.385832314475195	17.45257678485421	37.403671594235114	23.75791930643548
3	20.475343633062874	17.39700270460524	36.20651328220518	25.92114038012671
4	14.368215331036271	18.531640176355083	32.364954984994995	34.735189507613654
5	14.203808676966394	29.555685228409466	38.90972916898225	17.33077692564188
6	27.967655885295102	33.08324997221296	16.825052795376237	22.124041347115707
7	27.52630506465118	25.550646511800228	20.199788818495055	26.723259605053535
8	24.202146135427906	31.234282591479367	18.199080227673754	26.36449104541897
9	23.299509547384947	16.076564330803112	19.54539349675579	41.078532625056155
10-11	24.828812723319217	23.180042469728164	26.79344289627799	25.19770191067463
12-13	24.678693004191466	19.50536090591205	28.74279230252646	27.073153787370032
14-15	23.8033266719618	22.312970222974553	23.58681280002223	30.296890305041412
16-17	25.23747551142337	22.69182139433208	25.81825423658165	26.2524488576629
18-19	22.467840726203296	22.82978452418169	27.6327764076557	27.06959834195931
20-21	24.63464731395438	22.33357956479554	27.06654964883071	25.965223472419375
22-23	25.060670618747682	21.54061689514635	27.375185253797703	26.023527232308265
24-25	22.902787329771886	24.463293712819585	24.020775876641494	28.613143080767028
26-27	23.50936099758015	23.608702196389906	26.888582709073855	25.993354096956082
28-29	23.334745890269797	24.3703122213963	26.025551070880255	26.269390817453647
30-31	23.184549654381883	24.069841490384057	26.20375837993678	26.54185047529728
32-33	23.173236569771884	24.23774380967157	25.843188380962527	26.74583123959402
34-35	23.501282894432148	23.999157087412815	26.120795857686712	26.378764160468325
36-37	24.349402779771857	21.81949545427178	29.72878467188781	24.102317094068553
38-39	23.988152855032258	20.78854374597646	32.822334508167486	22.400968890823787
40-41	23.454940392934223	22.891290050668324	25.850570134405366	27.80319942199209
42-43	21.462247766544245	23.38818364123916	26.936703701816867	28.21286489039973
44-45	21.25103917486621	22.30908707764344	29.21847102930994	27.22140271818041
46-47	23.038572067033932	21.523894211063794	29.45181212529612	25.985721596606158
48-49	22.987893552181845	21.967135671875436	29.92825980233237	25.116710973610353
50-51	24.503288254909226	20.726658021489442	31.49870322341608	23.271350500185253
52-53	19.8644840226848	23.653003142433036	29.235742686374994	27.24677014850717
54-55	21.813554651407824	22.665033334336798	28.554987182663826	26.966424831591553
56-57	21.810885412324822	22.986078306054964	27.877659525198915	27.3253767564213
58-59	19.993284472078457	23.822987946785233	28.58777079208494	27.595956789051375
60-61	19.87096804073816	23.10880877009756	28.449092353258564	28.571130835905713
62-63	20.075121632668182	23.816614293025314	27.957103787806858	28.151160286499643
64-65	20.59646953392568	24.116515106254386	26.92472460928907	28.362290750530867
66-67	19.411760619316354	24.639731746910247	23.684033800259822	32.26447383351358
68-69	19.767404154390647	24.984018011006725	23.731400670780292	31.517177163822335
70-71	19.87897959372713	25.312856044536254	23.136758153709348	31.67140620802727
72-73	19.34293892069138	25.29131699364568	21.867068674855037	33.498675410807905
74-75	20.7073981789383	25.932761511314478	23.915328967478395	29.444511342268825
76	21.198058504233128	25.350599307137962	24.618370106893423	28.83297208173549
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	0.5
7	0.0
8	0.0
9	0.5
10	3.0
11	3.0
12	1.0
13	2.5
14	6.5
15	9.0
16	10.0
17	11.0
18	15.5
19	28.5
20	53.5
21	70.0
22	79.0
23	113.5
24	191.5
25	244.0
26	308.0
27	462.5
28	714.5
29	876.0
30	1082.0
31	1486.5
32	2015.0
33	2345.0
34	2710.0
35	3589.0
36	4693.5
37	5284.0
38	5981.5
39	7312.5
40	8726.0
41	10158.0
42	10810.0
43	11239.0
44	12050.0
45	12549.0
46	12666.0
47	12665.5
48	12423.0
49	11727.5
50	11274.0
51	10743.0
52	9604.0
53	8405.0
54	7814.0
55	7292.0
56	6381.0
57	5640.0
58	5288.0
59	5023.0
60	4492.0
61	4160.5
62	4095.0
63	3928.0
64	3568.0
65	3219.0
66	2934.5
67	2806.0
68	2700.5
69	2506.5
70	2332.0
71	2246.0
72	2125.0
73	1860.5
74	1589.0
75	1461.0
76	1347.0
77	1092.0
78	812.0
79	673.0
80	596.0
81	447.0
82	292.5
83	210.0
84	172.5
85	110.0
86	64.5
87	44.0
88	30.5
89	13.0
90	9.5
91	6.5
92	3.0
93	4.0
94	4.5
95	3.0
96	2.0
97	1.0
98	1.5
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.002315586677040495
9	0.002315586677040495
10-11	0.004399614686376941
12-13	0.006020525360305287
14-15	0.0030102626801526435
16-17	0.005094290689489089
18-19	0.004862732021785039
20-21	0.0030102626801526435
22-23	0.003704938683264792
24-25	0.002084028009336445
26-27	0.0025471453447445443
28-29	0.0034733800155607422
30-31	0.004862732021785039
32-33	0.0030102626801526435
34-35	0.00463117335408099
36-37	0.005094290689489089
38-39	0.005094290689489089
40-41	0.006483642695713386
42-43	0.003241821347856693
44-45	0.0053258493571931385
46-47	0.0034733800155607422
48-49	0.00463117335408099
50-51	0.003704938683264792
52-53	0.005788966692601237
54-55	0.004399614686376941
56-57	0.0027787040124485937
58-59	0.004862732021785039
60-61	0.005788966692601237
62-63	0.0053258493571931385
64-65	0.0034733800155607422
66-67	0.005788966692601237
68-69	0.027787040124485938
70-71	0.006483642695713386
72-73	0.005557408024897187
74-75	0.00463117335408099
76	0.005557408024897187
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	215928.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.9124660881972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.22512751755063	90.36739024824425
2	2.5760741673762277	4.838509557688016
3	0.6158890269019979	1.7351897205907993
4	0.25356143530454583	0.9525031877725108
5	0.12614839128639138	0.592345325938193
6	0.0813353157823016	0.4583040051106926
7	0.04002032855573688	0.26308854238304047
8	0.022119704667301298	0.16618528115590966
9	0.021570437344958206	0.1823159669099373
>10	0.03815367522988627	0.444168164206638
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00463117335408099	0.0
2	0.0	0.0	0.0	0.005557408024897188	0.0
3	0.0	0.0	0.0	0.006020525360305287	0.0
4	0.0	0.0	0.0	0.00879922937275388	0.0
5	0.0	0.0	0.0	0.010188581378978177	0.0
6	0.0	0.0	0.0	0.010188581378978177	0.0
7	0.0	0.0	0.0	0.010651698714386277	0.0
8	0.0	0.0	0.0	0.010651698714386277	0.0
9	0.0	0.0	0.0	0.015282872068467266	0.0
10	0.0	0.0	0.0	0.015745989403875366	0.0
11	0.0	0.0	0.0	0.015745989403875366	0.0
12	0.0	0.0	0.0	0.016672224074691565	0.0
13	0.0	0.0	0.0	0.017135341410099664	0.0
14	0.0	0.0	0.0	0.01898781075173206	0.0
15	0.0	0.0	0.0	0.01898781075173206	0.0
16	0.0	0.0	0.0	0.01945092808714016	0.0
17	0.0	0.0	0.0	0.020377162757956355	0.0
18	0.0	0.0	0.0	0.020840280093364454	0.0
19	0.0	0.0	0.0	0.021303397428772554	0.0
20	0.0	0.0	0.0	0.022229632099588753	0.0
21	0.0	0.0	0.0	0.02361898410581305	0.0
22	0.0	0.0	0.0	0.03149197880775073	0.0
23	0.0	0.0	0.0	0.03704938683264792	0.0
24	0.0	0.0	0.0	0.039364973509688414	0.0
25	0.0	0.0	0.0	0.04168056018672891	0.0
26	0.0	0.0	0.0	0.0477010855470342	0.0
27	0.0	0.0	0.0	0.08706605905672261	0.0
28	0.0	0.0	0.0	0.11994738987069764	0.0
29	0.0	0.0	0.0	0.14773442999518357	0.0
30	0.0	0.0	0.0	0.19450928087140157	0.0
31	0.0	0.0	0.0	0.21673891297099032	0.0
32	0.0	0.0	0.0	0.2690711718721055	0.0
33	0.0	0.0	0.0	0.3232559001148531	0.0
34	0.0	0.0	0.0	0.39781779111555704	0.0
35	0.0	0.0	0.0	0.542310399762884	0.0
36	0.0	0.0	0.0	0.6553110296024601	0.0
37	0.0	0.0	0.0	0.9716201696861917	0.0
38	0.0	0.0	0.0	1.2874661924345152	0.0
39	0.0	0.0	0.0	1.6977881516060909	0.0
40	0.0	0.0	0.0	2.230373087325405	0.0
41	0.0	0.0	0.0	2.89170464228817	0.0
42	0.0	0.0	0.0	3.602126634804194	0.0
43	0.0	0.0	0.0	4.364417768885925	0.0
44	0.0	0.0	0.0	5.061409358675114	0.0
45	0.0	0.0	0.0	5.488866659256789	0.0
46	0.0	0.0	0.0	6.070078915193953	0.0
47	0.0	0.0	0.0	6.7360416435107995	0.0
48	0.0	0.0	0.0	7.18433922418584	0.0
49	0.0	0.0	0.0	7.5858619539846615	0.0
50	0.0	0.0	0.0	8.175410321959172	0.0
51	0.0	0.0	0.0	8.90435700789152	0.0
52	0.0	0.0	0.0	9.536975288058983	0.0
53	0.0	0.0	0.0	10.398373531918047	0.0
54	0.0	0.0	0.0	11.178263124745285	0.0
55	0.0	0.0	0.0	11.652958393538587	0.0
56	0.0	0.0	0.0	12.18785891593494	0.0
57	0.0	0.0	0.0	12.590307880404579	0.0
58	0.0	0.0	0.0	12.961264866066466	0.0
59	0.0	0.0	0.0	13.393816457337632	0.0
60	0.0	0.0	0.0	13.669834389240858	0.0
61	0.0	0.0	0.0	13.94955725982735	0.0
62	0.0	0.0	0.0	14.170927346152421	0.0
63	0.0	0.0	0.0	14.49047830758401	0.0
64	0.0	0.0	0.0	14.831795783779778	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGATA	15	0.0022226549	69.99838	21
GATCTAG	25	2.3869486E-4	55.9987	41
ATCTCGG	175	0.0	49.998844	42
TCTCGGT	195	0.0	48.46042	43
GCGTGTA	65	1.8371793E-10	48.46042	34
GATCTCG	170	0.0	47.35184	41
GCGAGAA	30	5.86941E-4	46.665585	13
CGTGCGA	30	5.86941E-4	46.665585	10
CCGCGTA	30	5.86941E-4	46.665585	34
CTCGGTG	200	0.0	45.498943	44
TGCCTAC	85	1.8189894E-12	45.293068	19
GCCGTAT	125	0.0	44.79896	54
ATTTCGG	55	1.3062345E-7	44.544422	42
GTGTCGA	95	0.0	44.2095	36
CGCGTAG	40	4.582983E-5	43.74899	35
GCGTAGA	40	4.582983E-5	43.74899	36
TAGATCT	180	0.0	42.776783	39
AGATCTC	140	0.0	42.49902	40
CGGTGTT	75	7.512426E-10	41.999027	46
CGTATCA	135	0.0	41.480522	56
>>END_MODULE