FastQCFastQC Report
Wed 25 May 2016
SRR1779770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779770_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215928
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2338110.828146419176763No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG26441.2244822348190136No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC17340.8030454595976436No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT10360.47978955948279056Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC7380.34178059353117707No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC6100.2825015745989404Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC3740.17320588344262902Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT3260.15097625134304027No Hit
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT2260.10466451780223036Illumina PCR Primer Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGA308.357158E-658.33333237
CGTATGC35050.050.42796341
TATGCCG35050.050.42796343
CTCGTAT35150.050.38406839
TCGTATG35150.050.38406840
TCTCGTA33950.050.3092838
CGTCTTC35000.050.248
CCGTCTT34950.050.17167747
GTATGCC35300.050.0708242
ATGCCGT35350.049.90099344
GCCGTCT35350.049.90099346
ATCTCGT34250.049.86861437
TTGAAAA35350.049.80198358
TCTTCTG35400.049.7316450
CTTGAAA35450.049.661557
TGCCGTC35450.049.661545
GTCTTCT35500.049.5915549
CTTCTGC35700.049.31372551
TATCTCG34150.049.2972236
TGAAAAA35900.049.23398259
Produced by FastQC (version 0.11.5)