FastQCFastQC Report
Wed 25 May 2016
SRR1779769_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779769_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248057
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGAC305.8727746E-446.66290716
AGTCGCT305.8727746E-446.66290724
TCTCGGT1600.039.37182643
CGACGAT459.185407E-538.88575422
TCGATCT556.681692E-638.1787439
CAACTCG654.8526636E-737.68926669
GATCTCG1151.8189894E-1236.51879541
AGATCTC1201.8189894E-1234.9971840
CGCCGTA1400.034.99717753
GCCGTAT1003.237801E-1034.99717754
CTCGGTG1650.033.93665744
ATCTCGG1305.456968E-1232.30508842
AGGACGG652.0985732E-532.3050885
GATCTCT652.0985732E-532.30508841
TAGATCT1206.548362E-1132.08074639
TCTCTCG552.9881526E-431.81561741
GGACGGA552.9881526E-431.8156176
GCCGAAA1550.031.61035570
GCTGATA450.00430371131.10860454
TCGCTGA450.00430371131.10860452
Produced by FastQC (version 0.11.5)