##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779769_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248057
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.01670180643965	34.0	31.0	34.0	21.0	34.0
2	28.316657058659906	34.0	23.0	34.0	12.0	34.0
3	27.17114211652967	32.0	23.0	34.0	11.0	34.0
4	29.609448634789583	34.0	27.0	34.0	12.0	34.0
5	29.173198095598995	34.0	27.0	34.0	11.0	34.0
6	31.624211370773654	37.0	27.0	38.0	11.0	38.0
7	32.63917567333314	37.0	31.0	38.0	12.0	38.0
8	31.820130050754464	37.0	28.0	38.0	11.0	38.0
9	33.20778288861028	37.0	34.0	38.0	21.0	38.0
10-11	33.001015895540135	37.0	34.0	38.0	11.5	38.0
12-13	31.93933047646307	37.0	29.5	38.0	16.0	38.0
14-15	32.03575186348299	37.0	29.0	38.0	11.5	38.0
16-17	31.677991348762582	36.5	29.0	38.0	16.0	38.0
18-19	32.26280250103807	37.0	31.0	38.0	11.5	38.0
20-21	31.141584393909465	36.0	27.0	38.0	16.0	38.0
22-23	31.12895423229338	35.5	28.5	38.0	16.0	38.0
24-25	32.378453339353456	37.0	30.5	38.0	16.5	38.0
26-27	30.93787718145426	36.5	25.5	38.0	11.0	38.0
28-29	33.09510112595089	37.0	33.5	38.0	21.0	38.0
30-31	33.52632257908465	38.0	34.0	38.0	21.0	38.0
32-33	33.60816667136989	38.0	34.0	38.0	21.0	38.0
34-35	33.6728937300701	38.0	34.0	38.0	21.0	38.0
36-37	31.50848595282536	36.5	27.5	38.0	11.0	38.0
38-39	29.66351685298137	34.5	23.0	38.0	11.0	38.0
40-41	31.000076595298662	35.5	26.5	38.0	11.0	38.0
42-43	32.050526693461585	36.5	30.0	38.0	16.0	38.0
44-45	33.70980460136178	37.5	34.0	38.0	21.0	38.0
46-47	32.23436145724571	37.5	29.5	38.0	16.0	38.0
48-49	31.6922541996396	36.5	29.0	38.0	16.0	38.0
50-51	29.439985567833197	34.5	23.0	38.0	11.0	38.0
52-53	32.90715641969386	37.0	32.5	38.0	21.0	38.0
54-55	31.941646476414697	37.0	29.5	38.0	16.0	38.0
56-57	32.373660086189865	37.0	30.0	38.0	16.0	38.0
58-59	33.49179019338297	37.5	34.0	38.0	21.0	38.0
60-61	33.91662601740729	38.0	34.5	38.0	21.0	38.0
62-63	33.79680879797789	38.0	34.5	38.0	21.0	38.0
64-65	33.50973768125874	38.0	34.5	38.0	16.0	38.0
66-67	31.38367794498845	37.0	27.0	38.0	11.0	38.0
68-69	31.48981282527806	37.0	28.0	38.0	11.0	38.0
70-71	31.08619188331714	36.5	26.0	38.0	11.0	38.0
72-73	29.829053403048494	34.5	23.5	38.0	11.0	38.0
74-75	32.017379070133074	36.5	30.0	38.0	16.0	38.0
76	32.36392845192839	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	1.0
5	0.0
6	0.0
7	0.0
8	2.0
9	2.0
10	3.0
11	18.0
12	51.0
13	327.0
14	785.0
15	1472.0
16	2343.0
17	3728.0
18	4835.0
19	5436.0
20	4902.0
21	4079.0
22	3489.0
23	3277.0
24	3241.0
25	3542.0
26	4084.0
27	4945.0
28	5793.0
29	7014.0
30	8706.0
31	11339.0
32	14879.0
33	19511.0
34	26982.0
35	36520.0
36	42211.0
37	24536.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.23626021438621	17.742293101988658	10.985781493769577	29.035665189855557
2	22.117497188146274	17.496381879971135	35.69381230926763	24.692308622614963
3	21.347281852814902	17.158291507931708	34.71125355263954	26.783173086613854
4	15.140935917696005	18.413584029412714	32.13145418776405	34.31402586512723
5	14.97075268990595	29.445651604268374	37.91668850304567	17.666907202780006
6	28.84659574210766	31.732222835880464	16.977549514829253	22.443631907182624
7	28.38984588219643	26.684189520956874	19.9268716464361	24.999092950410592
8	24.620644058503864	30.645590440714045	18.1796558786061	26.554109622175996
9	23.81879605889088	16.09985003144502	19.88010578427104	40.201248125393064
10-11	25.609662684894396	23.182501703306226	26.218015134228594	24.98982047757078
12-13	25.367324682968917	20.12651006779053	27.578630361206802	26.92753488803375
14-15	23.904051602499496	22.342672848216086	23.6244708728079	30.128804676476516
16-17	24.7468150298339	23.068053539751652	25.978672794710526	26.206458635703918
18-19	22.864831631676022	23.066611568581997	27.212473668826913	26.856083130915064
20-21	24.159742631496194	22.747118512874472	27.307507790799402	25.785631064829932
22-23	24.573512307317063	22.304958606050953	27.594151832239106	25.52737725439288
24-25	23.602997794790546	24.352849638579475	23.84186189019105	28.202290676438928
26-27	23.196702311002714	23.608308724967497	27.085999939528932	26.10898902450086
28-29	23.825486445245808	24.372957816887357	25.663627622712404	26.137928115154434
30-31	23.52157392382354	24.32022738038844	25.840732938911675	26.31746575687634
32-33	23.598966333264798	24.255691415808972	25.72657015347774	26.41877209744849
34-35	23.483084688432925	24.163003009540628	26.16163631928948	26.192275982736966
36-37	23.885471008942034	22.2788882527959	29.554672192612543	24.28096854564953
38-39	23.70535516305772	21.15557508637685	32.19790276607497	22.94116698449047
40-41	23.62774702569333	22.47874343354526	25.763079490890618	28.130430049870785
42-43	21.701918367100113	22.56404467656657	26.99118522714216	28.74285172919115
44-45	21.70499350916377	22.06299739560236	29.224889333258076	27.007119761975794
46-47	22.46917412662545	21.320196011634916	30.240499261227228	25.9701306005124
48-49	22.437530740761645	21.944469081849043	30.321679393006022	25.29632078438329
50-51	22.916423097235604	21.146798792164386	32.27352074405253	23.66325736654747
52-53	19.798461550866385	23.76391901371542	29.13962151571105	27.297997919707147
54-55	21.371942308273784	22.849713254784966	28.962375399377123	26.815969037564123
56-57	21.198774415932593	23.2425567941301	28.19750448507327	27.36116430486404
58-59	19.66279904209771	23.546213947637902	28.765692906846418	28.02529410341797
60-61	20.032333299064874	23.189264699657112	28.58936911766781	28.189032883610203
62-63	20.179203160748663	23.95133898424665	27.761371539151558	28.108086315853132
64-65	20.846200362830075	23.791171134851844	26.49264261237654	28.869985889941546
66-67	19.89828212498841	23.553755659392273	23.657973141320518	32.889989074298796
68-69	20.349871157405726	23.926412530194334	23.659250656310867	32.06446565608908
70-71	20.28749163448126	24.174131800259634	23.231146338120155	32.30723022713895
72-73	19.7589147130561	24.079501703319963	21.918401902880525	34.24318168074341
74-75	21.07963192533688	25.766234289803364	23.505981717211423	29.648152067648333
76	21.836360337841924	25.235743514281683	23.95855590719426	28.969340240682133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.5
6	1.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	7.0
16	13.5
17	17.0
18	20.0
19	28.0
20	48.5
21	64.0
22	90.5
23	158.0
24	261.0
25	323.0
26	403.0
27	640.5
28	942.0
29	1086.0
30	1318.5
31	1803.0
32	2418.5
33	2782.0
34	3208.5
35	4210.0
36	5456.5
37	6128.0
38	6741.5
39	8230.5
40	9980.5
41	11351.5
42	11848.0
43	12288.0
44	13209.5
45	13930.5
46	14170.0
47	14181.0
48	13940.0
49	13124.5
50	12561.0
51	12036.5
52	10856.0
53	9674.0
54	9148.0
55	8546.5
56	7492.0
57	6655.5
58	6272.0
59	6020.0
60	5517.5
61	5057.5
62	4848.0
63	4662.5
64	4311.0
65	3985.5
66	3727.5
67	3629.0
68	3357.0
69	2964.5
70	2741.5
71	2639.0
72	2468.0
73	2148.5
74	1850.0
75	1700.0
76	1579.5
77	1281.0
78	946.5
79	790.0
80	701.5
81	513.0
82	347.5
83	282.0
84	233.5
85	137.5
86	88.0
87	86.0
88	64.5
89	37.5
90	20.0
91	7.0
92	6.0
93	4.5
94	4.5
95	3.5
96	1.0
97	0.5
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	8.062663016967875E-4
4	4.0313315084839374E-4
5	0.0
6	0.0
7	0.0
8	0.0020156657542419686
9	0.0020156657542419686
10-11	0.004031331508483937
12-13	0.005442297536453315
14-15	0.002821932055938756
16-17	0.0068532635644226935
18-19	0.005845430687301709
20-21	0.00322506520678715
22-23	0.005442297536453315
24-25	0.00322506520678715
26-27	0.0018140991788177716
28-29	0.003023498631362953
30-31	0.005845430687301709
32-33	0.00322506520678715
34-35	0.005039164385604921
36-37	0.006046997262725906
38-39	0.0064501304135743
40-41	0.0064501304135743
42-43	0.003023498631362953
44-45	0.006046997262725906
46-47	0.004232898083908134
48-49	0.006046997262725906
50-51	0.004031331508483937
52-53	0.006046997262725906
54-55	0.005845430687301709
56-57	0.0048375978101807245
58-59	0.006046997262725906
60-61	0.0062485638381501035
62-63	0.005845430687301709
64-65	0.002821932055938756
66-67	0.007256396715271086
68-69	0.0322506520678715
70-71	0.006046997262725906
72-73	0.0048375978101807245
74-75	0.0038297649330597404
76	0.0048375978101807245
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	248057.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.83671605686355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.25106106289486	93.20222325313354
2	1.9808467270914107	3.79675690672854
3	0.4241713979908491	1.219535814860756
4	0.15249023386006666	0.584566529755677
5	0.08000828214005094	0.3833865508826745
6	0.048790297227790054	0.28055411170498157
7	0.02438636146380463	0.16359761594866784
8	0.010160445111169729	0.07789949545044166
9	0.01136019685062972	0.09798515639314886
>10	0.01672499536936848	0.19349456514157523
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004434464659332331	0.0
2	0.0	0.0	0.0	0.005643864111877512	0.0
3	0.0	0.0	0.0	0.006046997262725905	0.0
4	0.0	0.0	0.0	0.022575456447510047	0.0
5	0.0	0.0	0.0	0.023381722749206836	0.0
6	0.0	0.0	0.0	0.023381722749206836	0.0
7	0.0	0.0	0.0	0.024591122201752018	0.0
8	0.0	0.0	0.0	0.02499425535260041	0.0
9	0.0	0.0	0.0	0.028622453710235952	0.0
10	0.0	0.0	0.0	0.029831853162781134	0.0
11	0.0	0.0	0.0	0.0322506520678715	0.0
12	0.0	0.0	0.0	0.032653785218719894	0.0
13	0.0	0.0	0.0	0.03305691836956828	0.0
14	0.0	0.0	0.0	0.03305691836956828	0.0
15	0.0	0.0	0.0	0.034266317822113465	0.0
16	0.0	0.0	0.0	0.034266317822113465	0.0
17	0.0	0.0	0.0	0.03628198357635543	0.0
18	0.0	0.0	0.0	0.037088249878052225	0.0
19	0.0	0.0	0.0	0.03950704878314258	0.0
20	0.0	0.0	0.0	0.041925847688232945	0.0
21	0.0	0.0	0.0	0.04394151344247491	0.0
22	0.0	0.0	0.0	0.049182244403504036	0.0
23	0.0	0.0	0.0	0.05079477700689761	0.0
24	0.0	0.0	0.0	0.055632374817078334	0.0
25	0.0	0.0	0.0	0.060469972627259054	0.0
26	0.0	0.0	0.0	0.0765952986611948	0.0
27	0.0	0.0	0.0	0.14593420060711854	0.0
28	0.0	0.0	0.0	0.19310077925638058	0.0
29	0.0	0.0	0.0	0.2237388987208585	0.0
30	0.0	0.0	0.0	0.2829994718955724	0.0
31	0.0	0.0	0.0	0.31202505875665676	0.0
32	0.0	0.0	0.0	0.36846369987543187	0.0
33	0.0	0.0	0.0	0.41764594427893587	0.0
34	0.0	0.0	0.0	0.48375978101807243	0.0
35	0.0	0.0	0.0	0.6180031202505876	0.0
36	0.0	0.0	0.0	0.7542621252373446	0.0
37	0.0	4.031331508483937E-4	0.0	1.1694892706111901	0.0
38	0.0	4.031331508483937E-4	0.0	1.5919728127003068	0.0
39	0.0	4.031331508483937E-4	0.0	2.149102827172787	0.0
40	0.0	4.031331508483937E-4	0.0	2.786859471814946	4.031331508483937E-4
41	0.0	4.031331508483937E-4	0.0	3.6745586699831088	4.031331508483937E-4
42	0.0	4.031331508483937E-4	4.031331508483937E-4	4.63038737064465	4.031331508483937E-4
43	0.0	4.031331508483937E-4	4.031331508483937E-4	5.667245834626719	4.031331508483937E-4
44	0.0	4.031331508483937E-4	4.031331508483937E-4	6.537610307308401	4.031331508483937E-4
45	0.0	4.031331508483937E-4	4.031331508483937E-4	7.124975308094511	4.031331508483937E-4
46	0.0	4.031331508483937E-4	4.031331508483937E-4	7.808285998782538	4.031331508483937E-4
47	0.0	4.031331508483937E-4	4.031331508483937E-4	8.570207653886001	4.031331508483937E-4
48	0.0	4.031331508483937E-4	4.031331508483937E-4	9.083799288066855	4.031331508483937E-4
49	0.0	4.031331508483937E-4	4.031331508483937E-4	9.519183090983121	4.031331508483937E-4
50	0.0	4.031331508483937E-4	4.031331508483937E-4	10.22224730606272	4.031331508483937E-4
51	0.0	4.031331508483937E-4	4.031331508483937E-4	11.079308384766405	4.031331508483937E-4
52	0.0	4.031331508483937E-4	4.031331508483937E-4	11.950479123749783	4.031331508483937E-4
53	0.0	4.031331508483937E-4	4.031331508483937E-4	13.110293198740612	4.031331508483937E-4
54	0.0	4.031331508483937E-4	4.031331508483937E-4	14.06450936679876	4.031331508483937E-4
55	0.0	4.031331508483937E-4	4.031331508483937E-4	14.643408571417053	4.031331508483937E-4
56	0.0	4.031331508483937E-4	4.031331508483937E-4	15.262621091120186	4.031331508483937E-4
57	0.0	4.031331508483937E-4	4.031331508483937E-4	15.750412203646743	4.031331508483937E-4
58	0.0	4.031331508483937E-4	4.031331508483937E-4	16.19063360437319	4.031331508483937E-4
59	0.0	4.031331508483937E-4	4.031331508483937E-4	16.698178241291316	4.031331508483937E-4
60	0.0	4.031331508483937E-4	4.031331508483937E-4	17.015444031009	4.031331508483937E-4
61	0.0	4.031331508483937E-4	4.031331508483937E-4	17.348028880458926	4.031331508483937E-4
62	0.0	4.031331508483937E-4	4.031331508483937E-4	17.599987099739174	4.031331508483937E-4
63	0.0	4.031331508483937E-4	4.031331508483937E-4	17.976513462631573	4.031331508483937E-4
64	0.0	4.031331508483937E-4	4.031331508483937E-4	18.37359961621724	4.031331508483937E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTGAC	30	5.8727746E-4	46.662907	16
AGTCGCT	30	5.8727746E-4	46.662907	24
TCTCGGT	160	0.0	39.371826	43
CGACGAT	45	9.185407E-5	38.885754	22
TCGATCT	55	6.681692E-6	38.17874	39
CAACTCG	65	4.8526636E-7	37.689266	69
GATCTCG	115	1.8189894E-12	36.518795	41
AGATCTC	120	1.8189894E-12	34.99718	40
CGCCGTA	140	0.0	34.997177	53
GCCGTAT	100	3.237801E-10	34.997177	54
CTCGGTG	165	0.0	33.936657	44
ATCTCGG	130	5.456968E-12	32.305088	42
AGGACGG	65	2.0985732E-5	32.305088	5
GATCTCT	65	2.0985732E-5	32.305088	41
TAGATCT	120	6.548362E-11	32.080746	39
TCTCTCG	55	2.9881526E-4	31.815617	41
GGACGGA	55	2.9881526E-4	31.815617	6
GCCGAAA	155	0.0	31.610355	70
GCTGATA	45	0.004303711	31.108604	54
TCGCTGA	45	0.004303711	31.108604	52
>>END_MODULE