FastQCFastQC Report
Wed 25 May 2016
SRR1779769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248057
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT201308.115070326578165No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG23420.9441378392869382No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC15130.6099404572336197No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT7360.29670599902441774RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC6200.2499425535260041No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4050.16326892609359944No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT3710.14956239896475407No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC2930.11811801319857936RNA PCR Primer, Index 24 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGT252.3873708E-456.00000437
GTTTGCC200.00694564352.521
CCGTATG200.00694564352.540
TCTCGTA27000.048.22222538
TATGCCG28450.048.10193343
CGTATGC28500.048.0175441
CCGTCTT28300.047.98586747
TCGTATG28300.047.98586740
CTCGTAT28250.047.946939
GCCGTCT28600.047.72727246
ATGCCGT28550.047.68826744
CGTCTTC28550.047.44308548
ATCTCGT27300.047.43589837
CATCTCG27200.047.3529436
GTATGCC28900.047.11072542
GTCTTCT28850.047.07105649
CTGCTTG28650.047.0331654
TCTTCTG28800.046.90972550
CTTGAAA28800.046.90972557
TGCCGTC29100.046.90721545
Produced by FastQC (version 0.11.5)