FastQCFastQC Report
Wed 25 May 2016
SRR1779768_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779768_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116230
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAAT150.00221986170.0000120
TTACGCG150.00221986170.0000114
ATTCGTG207.8987105E-570.032
ACGAGAG207.8987105E-570.024
ATCTAGG401.05592335E-861.2542
ATTTCGG308.332978E-658.33333642
CGGTGTT553.45608E-1157.27272446
TTCGTGT509.695214E-1056.00000433
TCTAGGT509.695214E-1056.00000443
TAGATCT1500.053.66666839
GTAGATC1250.053.20000538
GGCCAGT200.006936400752.511
GATCTAG407.4257514E-752.541
ATCGGTG200.006936400752.544
TCCGGTT200.006936400752.544
TCACGTG200.006936400752.544
CGTGTAT552.2573658E-950.9090935
GCCATAG352.0758001E-550.01
ATCTGGC352.0758001E-550.012
CGTATCA850.049.41176656
Produced by FastQC (version 0.11.5)