##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779768_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116230
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.447070463735695	32.0	23.0	34.0	12.0	34.0
2	26.705807450744214	32.0	21.0	34.0	12.0	34.0
3	25.877002495052913	31.0	21.0	34.0	11.0	34.0
4	27.33057730362213	32.0	23.0	34.0	12.0	34.0
5	27.14438613094726	32.0	23.0	34.0	11.0	34.0
6	29.059192979437324	34.0	21.0	38.0	11.0	38.0
7	30.081089219650693	35.0	25.0	38.0	12.0	38.0
8	29.357059279015743	34.0	21.0	38.0	11.0	38.0
9	30.755854770713242	36.0	26.0	38.0	12.0	38.0
10-11	30.22865009033812	36.0	24.5	38.0	11.0	38.0
12-13	29.735348016863117	35.5	24.0	38.0	11.0	38.0
14-15	29.353226361524563	34.5	22.0	38.0	11.0	38.0
16-17	29.14988815280048	34.5	23.5	38.0	11.0	38.0
18-19	29.6992514841263	34.5	23.0	38.0	11.0	38.0
20-21	28.662144024778456	33.0	22.0	37.5	11.0	38.0
22-23	28.68255613869053	33.5	23.0	37.5	11.0	38.0
24-25	29.497621096102556	35.0	22.5	38.0	11.0	38.0
26-27	28.32494192549256	33.5	21.0	38.0	11.0	38.0
28-29	29.948984771573606	36.0	23.0	38.0	11.0	38.0
30-31	30.388656112879637	37.0	24.0	38.0	11.0	38.0
32-33	30.51022971694055	37.0	25.0	38.0	11.0	38.0
34-35	30.55497720037856	37.0	25.0	38.0	11.0	38.0
36-37	28.916179127591846	34.5	22.0	38.0	11.0	38.0
38-39	27.715021939258367	31.5	21.0	37.0	11.0	38.0
40-41	28.350008603630734	34.0	21.0	37.0	11.0	38.0
42-43	29.192510539447646	35.0	22.5	38.0	11.0	38.0
44-45	30.565563967994493	37.0	26.0	38.0	11.0	38.0
46-47	29.449255785941666	35.5	23.5	38.0	11.0	38.0
48-49	28.826473371762884	35.0	22.0	38.0	11.0	38.0
50-51	27.120394906650606	31.0	19.5	37.0	11.0	38.0
52-53	29.66186870859503	35.0	23.0	38.0	11.0	38.0
54-55	28.95906392497634	35.0	22.0	38.0	11.0	38.0
56-57	29.356134388712036	35.5	22.5	38.0	11.0	38.0
58-59	30.181080616019962	36.5	23.0	38.0	11.0	38.0
60-61	30.6825346296137	37.0	25.0	38.0	11.0	38.0
62-63	30.63549427858556	37.0	24.5	38.0	11.0	38.0
64-65	30.413159253204853	37.0	24.0	38.0	11.0	38.0
66-67	28.738978749032093	34.0	21.0	38.0	11.0	38.0
68-69	28.724658005678396	34.0	21.0	38.0	11.0	38.0
70-71	28.46086638561473	34.0	21.0	38.0	11.0	38.0
72-73	27.497500645272304	32.0	21.0	37.0	11.0	38.0
74-75	28.88028047836187	35.0	22.0	37.5	11.0	38.0
76	29.00868106340876	34.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	1.0
5	0.0
6	0.0
7	0.0
8	1.0
9	0.0
10	0.0
11	13.0
12	76.0
13	396.0
14	1005.0
15	1749.0
16	2848.0
17	4419.0
18	5670.0
19	5876.0
20	4999.0
21	3848.0
22	2878.0
23	2241.0
24	1966.0
25	1835.0
26	1877.0
27	2052.0
28	2333.0
29	2880.0
30	3282.0
31	4396.0
32	5558.0
33	7385.0
34	9946.0
35	13339.0
36	15031.0
37	8325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.382431386044914	17.525595801428203	13.72451174395595	33.36746106857093
2	18.6303019874387	15.076142131979694	43.03019874386991	23.263357136711694
3	18.656433905771415	15.47303575730461	39.306363354785425	26.564166982138556
4	14.90579024348275	20.536006194614124	29.536264303536093	35.02193925836703
5	14.82835756689323	26.984427428374776	39.49152542372881	18.695689581003183
6	27.2150047319969	32.290286500903385	16.02254151251828	24.472167254581436
7	25.84788780865525	22.272218876365827	20.39404628753334	31.48584702744558
8	21.32950165198238	32.776362885462554	17.21159140969163	28.682544052863435
9	21.81305066079295	14.708666024229075	18.925523127753305	44.55276018722467
10-11	24.601300103681332	21.984744647074766	26.75795786493893	26.655997384304968
12-13	23.241427078000577	17.32911158242524	29.7642043132413	29.665257026332885
14-15	24.85125641544094	21.171277753638464	22.40037513927906	31.577090691641533
16-17	27.240069352125523	19.61305644110017	24.016830366938137	29.130043839836173
18-19	22.400664246011942	19.05233088399787	27.54771041627231	30.999294453717884
20-21	26.567978352240708	19.13236882068755	25.033448197238968	29.26620462983278
22-23	28.245634413598175	18.13390809789927	24.49159578907517	29.12886169942739
24-25	23.09052425079373	22.795401942816827	21.589960679039432	32.524113127350006
26-27	25.791144686123346	22.238522164096917	24.41019066629956	27.560142483480178
28-29	24.03710061131689	22.432017345740356	24.23499348244131	29.295888560501442
30-31	23.72991460345458	21.762997698380264	24.684549033104606	29.82253866506055
32-33	23.80370579099752	21.463817558411165	25.00613042973237	29.726346220858947
34-35	24.00579914647577	21.744218061674008	24.71864675110132	29.5313360407489
36-37	26.496300120461196	18.098864223025295	29.424367578729992	25.980468077783513
38-39	25.680814999010504	16.771065470095767	33.49394687707041	24.05417265382332
40-41	24.612915854639635	22.06648511678132	24.325533571671336	28.995065456907714
42-43	20.473396200440526	22.703572411894275	26.541420016519822	30.281611371145374
44-45	20.457488502557617	19.38668834940179	29.41194185241113	30.743881295629468
46-47	25.246293762852446	18.82114555638729	27.848618603890795	28.083942076869466
48-49	24.80306999995698	19.20341761205973	29.41452313040186	26.57898925758143
50-51	29.955086729074893	17.705035104625548	28.486801349118945	23.853076817180614
52-53	19.78997272484792	21.463909897352504	28.850571745695774	29.895545632103797
54-55	24.18969033135148	19.85570593954621	26.559313721272403	29.39529000782991
56-57	23.967080809471366	20.296152257709252	26.18435090859031	29.55241602422907
58-59	20.354922669878896	22.101572414979888	27.823356062724514	29.7201488524167
60-61	19.989588755856325	20.705899561609183	27.63367908415469	31.670832598379807
62-63	20.230940324641523	21.999991395665997	27.231856686212847	30.537211593479636
64-65	20.548770257305968	23.11838827775794	26.12294415501168	30.20989730992441
66-67	19.24298092394531	27.033875700186716	21.05507705280548	32.6680663230625
68-69	19.580362406461525	26.954572251076847	21.396697806713686	32.068367535747946
70-71	18.9244536224402	27.86009292720702	20.6078988125968	32.60755463775598
72-73	18.205166813654845	28.651939168405427	19.738862956828495	33.404031061111226
74-75	20.380472191629956	25.320501789647576	22.712606690528634	31.58641932819383
76	21.44602570941818	23.8190703997522	23.72012183579701	31.014782055032608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	1.5
6	1.5
7	1.0
8	0.0
9	0.5
10	0.5
11	0.5
12	1.0
13	0.5
14	0.0
15	0.5
16	1.5
17	2.0
18	4.0
19	8.0
20	19.0
21	28.0
22	32.0
23	51.5
24	87.0
25	107.0
26	142.5
27	215.0
28	331.0
29	410.0
30	526.0
31	696.5
32	930.5
33	1110.0
34	1338.5
35	1783.0
36	2267.0
37	2535.0
38	2804.0
39	3437.5
40	4010.0
41	4495.0
42	4772.0
43	5014.0
44	5410.5
45	5665.5
46	5766.0
47	5720.5
48	5551.0
49	5224.5
50	5022.0
51	4767.0
52	4273.0
53	3905.0
54	3776.0
55	3573.0
56	3289.0
57	3169.5
58	3131.0
59	3105.0
60	3024.0
61	2930.5
62	2892.0
63	2888.5
64	2836.0
65	2710.5
66	2622.5
67	2611.0
68	2567.5
69	2398.0
70	2219.0
71	2166.0
72	2048.5
73	1824.5
74	1590.5
75	1463.0
76	1342.5
77	1068.0
78	780.0
79	646.0
80	553.0
81	386.0
82	253.5
83	195.0
84	156.0
85	88.5
86	43.5
87	27.0
88	19.5
89	9.5
90	4.0
91	1.5
92	2.0
93	1.5
94	2.0
95	2.0
96	1.0
97	0.5
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0017207261464337949
4	0.0
5	0.0
6	0.0
7	0.0
8	0.005162178439301385
9	0.005162178439301385
10-11	0.007313086122343629
12-13	0.005592359975909835
14-15	0.005592359975909835
16-17	0.009894175341994322
18-19	0.0068829045857351796
20-21	0.0047319969026929365
22-23	0.007313086122343629
24-25	0.006022541512518282
26-27	0.005162178439301385
28-29	0.0047319969026929365
30-31	0.006452723049126731
32-33	0.005592359975909835
34-35	0.005162178439301385
36-37	0.008603630732168976
38-39	0.007743267658952078
40-41	0.007313086122343629
42-43	0.005162178439301385
44-45	0.007313086122343629
46-47	0.006022541512518282
48-49	0.007313086122343629
50-51	0.005162178439301385
52-53	0.006022541512518282
54-55	0.007743267658952078
56-57	0.005162178439301385
58-59	0.006452723049126731
60-61	0.008173449195560527
62-63	0.008173449195560527
64-65	0.005592359975909835
66-67	0.009463993805385873
68-69	0.029682526025982967
70-71	0.008603630732168976
72-73	0.006452723049126731
74-75	0.005162178439301385
76	0.0068829045857351796
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	116230.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.90983874817812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.9157792356234	92.9517675864267
2	2.355739213084034	4.518771361192994
3	0.4855320834970248	1.3970191150579978
4	0.13897980380576236	0.533181222890564
5	0.05498438167101815	0.26367715898678157
6	0.024992513345519624	0.14382167549282862
7	0.01299608442575081	0.08725166531420282
8	0.0049984930422345055	0.038352372933168136
9	0.0	0.0
>10	0.00599819150524949	0.06615784170476684
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0025810892196506926	0.0
2	0.0	0.0	0.0	0.0025810892196506926	0.0
3	0.0	0.0	0.0	0.0025810892196506926	0.0
4	0.0	0.0	0.0	0.01032435687860277	0.0
5	0.0	0.0	0.0	0.01032435687860277	0.0
6	0.0	0.0	0.0	0.01032435687860277	0.0
7	0.0	0.0	0.0	0.01032435687860277	0.0
8	0.0	0.0	0.0	0.01032435687860277	0.0
9	0.0	0.0	0.0	0.011184719951819667	0.0
10	0.0	0.0	0.0	0.012905446098253464	0.0
11	0.0	0.0	0.0	0.01376580917147036	0.0
12	0.0	0.0	0.0	0.01376580917147036	0.0
13	0.0	0.0	0.0	0.016346898391121054	0.0
14	0.0	0.0	0.0	0.018927987610771746	0.0
15	0.0	0.0	0.0	0.018927987610771746	0.0
16	0.0	0.0	0.0	0.018927987610771746	0.0
17	0.0	0.0	0.0	0.021509076830422438	0.0
18	0.0	0.0	0.0	0.021509076830422438	0.0
19	0.0	0.0	0.0	0.026671255269723824	8.603630732168976E-4
20	0.0	0.0	0.0	0.029252344489374516	8.603630732168976E-4
21	0.0	0.0	0.0	0.029252344489374516	8.603630732168976E-4
22	0.0	0.0	0.0	0.04559924288049557	8.603630732168976E-4
23	0.0	0.0	0.0	0.04990105824658006	8.603630732168976E-4
24	0.0	0.0	0.0	0.05592359975909834	8.603630732168976E-4
25	0.0	0.0	0.0	0.06108577819839973	8.603630732168976E-4
26	0.0	0.0	0.0	0.06280650434483352	8.603630732168976E-4
27	0.0	0.0	0.0	0.11528865181106426	8.603630732168976E-4
28	0.0	0.0	0.0	0.1755140669362471	8.603630732168976E-4
29	0.0	0.0	0.0	0.21164931601135678	8.603630732168976E-4
30	0.0	0.0	0.0	0.2839198141615762	8.603630732168976E-4
31	0.0	0.0	0.0	0.3062892540652155	8.603630732168976E-4
32	0.0	0.0	0.0	0.3716768476296997	8.603630732168976E-4
33	0.0	0.0	0.0	0.4319022627548826	8.603630732168976E-4
34	0.0	0.0	0.0	0.5394476469069948	0.001720726146433795
35	0.0	0.0	0.0	0.7648627720898219	0.001720726146433795
36	0.0	0.0	0.0	0.9162866729759959	0.001720726146433795
37	0.0	0.0	0.0	1.343887120364794	0.001720726146433795
38	0.0	0.0	0.0	1.7009377957498064	0.001720726146433795
39	0.0	0.0	0.0	2.1724167598726662	0.001720726146433795
40	0.0	0.0	0.0	2.6748687946313345	0.001720726146433795
41	0.0	0.0	0.0	3.23066333992945	0.001720726146433795
42	0.0	0.0	0.0	3.7073044824916113	0.001720726146433795
43	0.0	0.0	0.0	4.220080874128882	0.001720726146433795
44	0.0	0.0	0.0	4.793943043964553	0.001720726146433795
45	0.0	0.0	0.0	5.165619891594253	0.001720726146433795
46	0.0	0.0	0.0	5.591499612836617	0.001720726146433795
47	0.0	0.0	0.0	6.085348016863116	0.001720726146433795
48	0.0	0.0	0.0	6.438096876882044	0.001720726146433795
49	0.0	0.0	0.0	6.817516992170696	0.001720726146433795
50	0.0	0.0	0.0	7.281252688634604	0.001720726146433795
51	0.0	0.0	0.0	7.7613352834896325	0.001720726146433795
52	0.0	0.0	0.0	8.17258883248731	0.001720726146433795
53	0.0	0.0	0.0	8.761077174567667	0.001720726146433795
54	0.0	0.0	0.0	9.22911468639766	0.001720726146433795
55	0.0	0.0	0.0	9.544867934268261	0.001720726146433795
56	0.0	0.0	0.0	9.919986234190828	0.001720726146433795
57	0.0	0.0	0.0	10.246924202013249	0.001720726146433795
58	0.0	0.0	0.0	10.520519659296223	0.001720726146433795
59	0.0	0.0	0.0	10.827669276434655	0.001720726146433795
60	0.0	0.0	0.0	11.018669878688806	0.001720726146433795
61	0.0	0.0	0.0	11.232900283919815	0.001720726146433795
62	0.0	0.0	0.0	11.38948636324529	0.001720726146433795
63	0.0	0.0	0.0	11.604577131549513	0.001720726146433795
64	0.0	0.0	0.0	11.823969715219823	0.001720726146433795
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGAAT	15	0.002219861	70.00001	20
TTACGCG	15	0.002219861	70.00001	14
ATTCGTG	20	7.8987105E-5	70.0	32
ACGAGAG	20	7.8987105E-5	70.0	24
ATCTAGG	40	1.05592335E-8	61.25	42
ATTTCGG	30	8.332978E-6	58.333336	42
CGGTGTT	55	3.45608E-11	57.272724	46
TTCGTGT	50	9.695214E-10	56.000004	33
TCTAGGT	50	9.695214E-10	56.000004	43
TAGATCT	150	0.0	53.666668	39
GTAGATC	125	0.0	53.200005	38
GGCCAGT	20	0.0069364007	52.5	11
GATCTAG	40	7.4257514E-7	52.5	41
ATCGGTG	20	0.0069364007	52.5	44
TCCGGTT	20	0.0069364007	52.5	44
TCACGTG	20	0.0069364007	52.5	44
CGTGTAT	55	2.2573658E-9	50.90909	35
GCCATAG	35	2.0758001E-5	50.0	1
ATCTGGC	35	2.0758001E-5	50.0	12
CGTATCA	85	0.0	49.411766	56
>>END_MODULE