FastQCFastQC Report
Wed 25 May 2016
SRR1779768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116230
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2422920.845736900972213No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG29442.5329088875505463No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC17861.5366084487653788No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT9670.8319710918007398TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6930.59623160973931No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC5830.5015916716854513TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT3550.3054288909919986No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC3490.30026671255269727RNA PCR Primer, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2490.2142304052310075No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2130.1832573345951992TruSeq Adapter, Index 12 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1820.15658607932547533No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1820.15658607932547533No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT1730.14884281166652327RNA PCR Primer, Index 12 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG1240.1066850210788953No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT1180.10152284263959391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAGC252.8249415E-670.0000133
ATCGTAT150.00221986170.0000139
GAAAGCT252.8249415E-670.0000134
TTGAAAG252.8249415E-670.0000132
CTTGTGT207.8987105E-570.067
AAGGGTC207.8987105E-570.033
TCTTTAT1100.060.4545441
CTTTATA1150.057.8260842
AGGGTCA252.3820717E-456.00000434
GTAATCA252.3820717E-456.00000452
CTAGTGT252.3820717E-456.0000044
CAAGGGT252.3820717E-456.00000432
GGAGTAA252.3820717E-456.00000449
AGGTGTT252.3820717E-456.00000460
AGTAATC252.3820717E-456.00000451
GATCCGA200.006936400752.511
GGGACAC200.006936400752.549
TTTATAC1400.052.53
AACCTAT200.006936400752.570
TATCGTA200.006936400752.538
Produced by FastQC (version 0.11.5)