##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779768_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116230
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.6332702400413	34.0	34.0	34.0	34.0	34.0
2	33.645754108233675	34.0	34.0	34.0	34.0	34.0
3	33.65582035619031	34.0	34.0	34.0	34.0	34.0
4	33.69550890475781	34.0	34.0	34.0	34.0	34.0
5	33.70266712552697	34.0	34.0	34.0	34.0	34.0
6	37.35819495827239	38.0	38.0	38.0	37.0	38.0
7	37.35004731996903	38.0	38.0	38.0	37.0	38.0
8	37.21945280908543	38.0	38.0	38.0	36.0	38.0
9	37.2516992170696	38.0	38.0	38.0	36.0	38.0
10-11	37.14874817172847	38.0	38.0	38.0	36.0	38.0
12-13	37.14157704551321	38.0	38.0	38.0	36.0	38.0
14-15	37.15031403252172	38.0	38.0	38.0	36.0	38.0
16-17	37.07446872580229	38.0	38.0	38.0	35.5	38.0
18-19	37.057003355415986	38.0	38.0	38.0	35.0	38.0
20-21	37.10853049987095	38.0	38.0	38.0	36.0	38.0
22-23	37.076206659210186	38.0	38.0	38.0	35.5	38.0
24-25	37.02642605179386	38.0	38.0	38.0	35.0	38.0
26-27	36.9728813559322	38.0	38.0	38.0	35.0	38.0
28-29	36.86987438699131	38.0	37.5	38.0	34.5	38.0
30-31	36.658595027101434	38.0	37.0	38.0	34.0	38.0
32-33	36.679303966273764	38.0	37.0	38.0	34.0	38.0
34-35	36.49217499784909	38.0	37.0	38.0	34.0	38.0
36-37	36.53306375290373	38.0	37.0	38.0	34.0	38.0
38-39	35.85628925406522	38.0	37.0	38.0	32.5	38.0
40-41	36.58148498666437	38.0	37.0	38.0	34.0	38.0
42-43	36.87157360406091	38.0	37.0	38.0	35.0	38.0
44-45	36.861993461240644	38.0	37.0	38.0	35.0	38.0
46-47	36.87936419168889	38.0	37.5	38.0	35.0	38.0
48-49	36.84809429579282	38.0	37.0	38.0	34.5	38.0
50-51	36.738806676417454	38.0	37.0	38.0	34.0	38.0
52-53	36.73080529983653	38.0	37.0	38.0	34.0	38.0
54-55	36.73882818549428	38.0	37.0	38.0	34.0	38.0
56-57	36.74900197883507	38.0	37.0	38.0	34.0	38.0
58-59	36.63180332100146	38.0	37.0	38.0	34.0	38.0
60-61	36.66737073044825	38.0	37.0	38.0	34.0	38.0
62-63	36.723384668330034	38.0	37.0	38.0	34.0	38.0
64-65	36.75731738793771	38.0	37.0	38.0	34.5	38.0
66-67	36.65830250365654	38.0	37.0	38.0	34.0	38.0
68-69	36.36350339843414	38.0	37.0	38.0	34.0	38.0
70-71	34.46623074937624	38.0	34.0	38.0	26.5	38.0
72-73	31.290875849608533	38.0	24.0	38.0	11.0	38.0
74-75	30.91050933493934	38.0	23.5	38.0	11.0	38.0
76	30.601488428116664	37.0	24.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	2.0
21	8.0
22	7.0
23	22.0
24	39.0
25	97.0
26	134.0
27	286.0
28	491.0
29	790.0
30	1346.0
31	1976.0
32	3130.0
33	4915.0
34	8730.0
35	19007.0
36	21704.0
37	53545.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.579024348274974	13.548137313946484	11.229458831626946	44.6433795061516
2	16.574034242450313	12.948464251914308	47.52903725372107	22.94846425191431
3	15.928761937537642	15.406521552094985	44.743181622644755	23.921534887722622
4	12.441710401789555	33.182483007829305	28.342080357911037	26.0337262324701
5	12.871031575324787	26.990449969887294	46.475092489030374	13.66342596575755
6	24.651983136883764	46.95173363159253	13.770971349909662	14.625311881614042
7	22.708422954486792	22.54237288135593	15.888324873096446	38.860879291060826
8	19.660156586079324	46.46390776907855	13.174739740170352	20.70119590467177
9	18.326593822593136	14.24589176632539	13.257334595199174	54.1701798158823
10-11	21.263873354555624	29.302675729157706	27.580228856577477	21.8532220597092
12-13	20.4633055149273	14.449367633141186	30.154004990105825	34.93332186182569
14-15	19.80813903467263	14.488944334509164	16.888927127247698	48.813989503570504
16-17	33.26292695517508	27.188333476727177	14.685107115202614	24.86363245289512
18-19	33.90432762625828	18.44962574206315	14.809429579282456	32.83661705239611
20-21	20.767013679772862	16.124924718231092	14.974619289340103	48.13344231265594
22-23	20.949840832831455	28.678482319538844	14.914393874214918	35.457282973414785
24-25	33.105910694313	28.769250623763227	14.842983739137916	23.281854942785856
26-27	33.29476038888411	30.625483954228685	15.699475178525338	20.38028047836187
28-29	20.691301729329776	18.795491697496345	27.78886690183257	32.724339671341305
30-31	19.831799019186096	27.85081304310419	19.574550460294244	32.74283747741547
32-33	19.310849178353266	17.727350942097566	40.19616278069345	22.765637098855716
34-35	32.25157016260862	27.93857007657231	20.616880323496513	19.19297943732255
36-37	20.653445754108233	42.74972038200121	16.593392411597694	20.003441452292865
38-39	18.95164759528521	19.43431127935989	29.513034500559236	32.101006624795666
40-41	19.186526714273423	15.080013765809172	31.39765981244085	34.33579970747655
42-43	31.728469414092746	14.884711348188937	30.55923599759098	22.827583240127332
44-45	19.155983825174225	27.59829648111503	32.510539447646906	20.735180246063837
46-47	30.854340531704377	16.171384324184807	21.352060569560354	31.622214574550462
48-49	30.20734750064527	14.896326249677363	18.825174223522325	36.07115202615504
50-51	19.441194183945626	15.501161490148844	31.740944678654394	33.31669964725114
52-53	16.58091714703605	15.51578766239353	44.18824744041986	23.715047750150564
54-55	16.656629097479136	15.360922309214489	34.12974275144111	33.852705841865266
56-57	29.066936247096276	15.602684332788435	21.804181364535836	33.52619805557946
58-59	18.316699647251138	16.412716166222147	41.81192463219479	23.45865955433193
60-61	29.283747741546932	28.81614041125355	20.677105738621698	21.22300610857782
62-63	17.297169405489115	45.18110642691215	16.22257592704121	21.299148240557518
64-65	16.215693022455476	46.70308870343285	15.76572313516304	21.315495138948638
66-67	16.305170782070032	46.806332272218874	15.373397573776135	21.515099371934955
68-69	16.241073733115375	47.14574550460294	15.352318678482318	21.260862083799363
70-71	16.59253204852448	44.62014970317474	16.349909661877312	22.437408586423473
72-73	16.922911468639764	37.520003441452296	18.965843585993287	26.591241503914652
74-75	17.4541856663512	34.6179987954917	20.74550460294244	27.18231093521466
76	17.59528521035877	35.55106254839542	19.91998623419083	26.93366600705498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	0.5
16	1.0
17	2.0
18	2.0
19	6.0
20	15.5
21	21.0
22	27.0
23	46.5
24	71.0
25	82.0
26	123.5
27	160.0
28	255.5
29	356.0
30	387.5
31	449.5
32	623.0
33	766.0
34	921.5
35	1177.0
36	1577.5
37	1878.0
38	6681.0
39	12593.5
40	12214.5
41	9124.0
42	7522.0
43	6303.0
44	4616.0
45	3907.0
46	3666.0
47	3537.0
48	3287.5
49	3021.5
50	2876.0
51	2618.5
52	2200.0
53	2001.0
54	1963.0
55	1880.5
56	1810.5
57	1794.5
58	1766.0
59	1825.5
60	1879.0
61	1918.5
62	1964.0
63	2002.5
64	2030.0
65	2097.5
66	2156.5
67	2137.0
68	2182.0
69	2173.5
70	2052.5
71	1985.0
72	1928.0
73	1943.0
74	1882.5
75	1750.0
76	1629.0
77	1405.5
78	1170.0
79	1037.0
80	883.5
81	639.0
82	435.0
83	322.0
84	265.0
85	156.0
86	78.0
87	52.0
88	38.0
89	16.5
90	6.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	116230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.689753075797988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.84568186137431	15.162178439301385
2	24.29071756305487	15.395336832143164
3	13.205549371487525	12.554417964380967
4	6.323134146010371	8.015142390088617
5	3.089620720549507	4.895465886604147
6	1.6371188879537373	3.1127935988987354
7	0.9529498004506828	2.1139120708939174
8	0.6000054299133929	1.521121913447475
9	0.4724024651806803	1.3473285726576614
>10	1.523090706703228	6.876021681149445
>50	0.01628974017864415	0.3888841090940377
>100	0.027149566964406915	1.7723479308268089
>500	0.008144870089322074	1.9297943732255012
>1k	0.005429913392881383	4.069517336315926
>5k	0.0	0.0
>10k+	0.0027149566964406917	20.845736900972213
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	24229	20.845736900972213	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	2944	2.5329088875505463	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	1786	1.5366084487653788	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	967	0.8319710918007398	TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	693	0.59623160973931	No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	583	0.5015916716854513	TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT	355	0.3054288909919986	No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	349	0.30026671255269727	RNA PCR Primer, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	249	0.2142304052310075	No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	213	0.1832573345951992	TruSeq Adapter, Index 12 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	182	0.15658607932547533	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	182	0.15658607932547533	No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT	173	0.14884281166652327	RNA PCR Primer, Index 12 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG	124	0.1066850210788953	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT	118	0.10152284263959391	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0025810892196506926	0.0
2	0.0	0.0	0.0	0.0025810892196506926	0.0
3	0.0	0.0	0.0	0.0025810892196506926	0.0
4	0.0	0.0	0.0	0.01032435687860277	0.0
5	0.0	0.0	0.0	0.01376580917147036	0.0
6	0.0	0.0	0.0	0.01376580917147036	0.0
7	0.0	0.0	0.0	0.01376580917147036	0.0
8	0.0	0.0	0.0	0.01376580917147036	0.0
9	0.0	0.0	0.0	0.014626172244687258	0.0
10	0.0	0.0	0.0	0.016346898391121054	0.0
11	0.0	0.0	0.0	0.01806762453755485	0.0
12	0.0	0.0	0.0	0.01806762453755485	0.0
13	0.0	0.0	0.0	0.021509076830422438	0.0
14	0.0	0.0	0.0	0.02753161834294072	0.0
15	0.0	0.0	0.0	0.029252344489374516	0.0
16	0.0	0.0	0.0	0.029252344489374516	0.0
17	0.0	0.0	0.0	0.033554159855459	0.0
18	0.0	0.0	0.0	0.03699561214832659	0.0
19	0.0	0.0	0.0	0.04129742751441108	0.0
20	0.0	0.0	0.0	0.046459605953712464	0.0
21	0.0	0.0	0.0	0.047319969026929365	0.0
22	0.0	0.0	0.0	0.05592359975909834	0.0
23	0.0	0.0	0.0	0.06022541512518283	0.0
24	0.0	0.0	0.0	0.06366686741805042	0.0
25	0.0	0.0	0.0	0.0688290458573518	0.0
26	0.0	0.0	0.0	0.0722704981502194	0.0
27	0.0	0.0	0.0	0.14368063322722188	0.0
28	0.0	0.0	0.0	0.22283403596317647	0.0
29	0.0	0.0	0.0	0.29940634947948036	0.0
30	0.0	0.0	0.0	0.3828615675815194	0.0
31	0.0	0.0	0.0	0.4654564226103416	0.0
32	0.0	0.0	0.0	0.5764432590553213	0.0
33	0.0	0.0	0.0	0.7115202615503743	0.0
34	0.0	0.0	0.0	0.9403768390260691	0.0
35	0.0	0.0	0.0	1.2784995268003096	0.0
36	0.0	0.0	0.0	1.7181450572141443	0.0
37	0.0	0.0	0.0	2.3556740944678656	0.0
38	0.0	0.0	0.0	2.9794373225501163	0.0
39	0.0	0.0	0.0	3.6668674180504173	0.0
40	0.0	0.0	0.0	4.403338208724081	0.0
41	0.0	0.0	0.0	5.188849694571109	0.0
42	0.0	0.0	0.0	5.853049987094554	0.0
43	0.0	0.0	0.0	6.563709885571711	0.0
44	0.0	0.0	0.0	7.313086122343629	0.0
45	0.0	0.0	0.0	8.030628925406521	0.0
46	0.0	0.0	0.0	8.729243740858642	0.0
47	0.0	0.0	0.0	9.402908027187474	0.0
48	0.0	0.0	0.0	10.054202873612665	0.0
49	0.0	0.0	0.0	10.752817689064786	0.0
50	0.0	0.0	0.0	11.3034500559236	0.0
51	0.0	0.0	0.0	11.856663512002065	0.0
52	0.0	0.0	0.0	12.435687860277037	0.0
53	0.0	0.0	0.0	13.056869999139638	0.0
54	0.0	0.0	0.0	13.567065301557257	0.0
55	0.0	0.0	0.0	14.069517336315926	0.0
56	0.0	0.0	0.0	14.593478447905015	0.0
57	0.0	0.0	0.0	15.129484642519143	0.0
58	0.0	0.0	0.0	15.618170868106342	0.0
59	0.0	0.0	0.0	16.052654220080875	0.0
60	0.0	0.0	0.0	16.480254667469673	0.0
61	0.0	0.0	0.0	16.931084917835328	0.0
62	0.0	0.0	0.0	17.392239525079585	0.0
63	0.0	0.0	0.0	17.86543921534888	0.0
64	0.0	0.0	0.0	18.340359631764606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAAAGC	25	2.8249415E-6	70.00001	33
ATCGTAT	15	0.002219861	70.00001	39
GAAAGCT	25	2.8249415E-6	70.00001	34
TTGAAAG	25	2.8249415E-6	70.00001	32
CTTGTGT	20	7.8987105E-5	70.0	67
AAGGGTC	20	7.8987105E-5	70.0	33
TCTTTAT	110	0.0	60.454544	1
CTTTATA	115	0.0	57.826084	2
AGGGTCA	25	2.3820717E-4	56.000004	34
GTAATCA	25	2.3820717E-4	56.000004	52
CTAGTGT	25	2.3820717E-4	56.000004	4
CAAGGGT	25	2.3820717E-4	56.000004	32
GGAGTAA	25	2.3820717E-4	56.000004	49
AGGTGTT	25	2.3820717E-4	56.000004	60
AGTAATC	25	2.3820717E-4	56.000004	51
GATCCGA	20	0.0069364007	52.5	11
GGGACAC	20	0.0069364007	52.5	49
TTTATAC	140	0.0	52.5	3
AACCTAT	20	0.0069364007	52.5	70
TATCGTA	20	0.0069364007	52.5	38
>>END_MODULE