FastQCFastQC Report
Wed 25 May 2016
SRR1779767_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779767_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184406
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGAG150.002223130569.9905119
TCTGGAC150.002223130569.990513
TCGGTTC200.006939104852.50712252
TACCCAG200.00694655352.4928865
ACGTGTA200.00694655352.49288634
CAGTGTA407.4567834E-752.49288634
GATTCGA200.00694655352.49288623
CGACTAA552.2719178E-950.9021922
ATCTCGG1550.047.41292642
GCCGTAC305.862578E-446.67299354
CGTGTAT451.6796148E-646.66033635
ACGCTTC1250.044.79392615
GACGCTT950.044.2045314
TCTCGGT2000.043.74407243
AGATCTC1450.043.44238740
GTGTCGC659.958967E-943.07108336
GTAGATC1250.041.99430538
CCGACTA1100.041.35803221
CGGTGGT2150.040.69215446
CGCCGTA953.6379788E-1240.5318153