##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779767_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	184406
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.94138477056061	34.0	23.0	34.0	12.0	34.0
2	27.6050616574298	33.0	23.0	34.0	12.0	34.0
3	26.568506447729465	32.0	21.0	34.0	11.0	34.0
4	28.733229938288343	33.0	23.0	34.0	12.0	34.0
5	28.399184408316433	34.0	23.0	34.0	11.0	34.0
6	30.539667906684166	36.0	26.0	38.0	11.0	38.0
7	31.58133141004089	37.0	27.0	38.0	12.0	38.0
8	30.763576022472154	37.0	26.0	38.0	11.0	38.0
9	32.255517716343284	37.0	31.0	38.0	12.0	38.0
10-11	31.899780918191382	37.0	28.0	38.0	11.0	38.0
12-13	31.07900231012006	36.0	27.0	38.0	11.0	38.0
14-15	30.96516653471145	36.5	26.5	38.0	11.0	38.0
16-17	30.68579655759574	35.5	26.5	38.0	11.0	38.0
18-19	31.255327917746712	36.5	26.5	38.0	11.5	38.0
20-21	30.14191512206761	35.5	24.5	38.0	11.0	38.0
22-23	30.14714271769899	35.5	26.0	38.0	11.0	38.0
24-25	31.19201381733783	36.5	27.0	38.0	11.0	38.0
26-27	29.86187271563832	35.0	24.0	38.0	11.0	38.0
28-29	31.820119735800354	37.0	29.0	38.0	11.0	38.0
30-31	32.23938483563442	37.0	31.0	38.0	11.0	38.0
32-33	32.36024587052482	37.0	31.0	38.0	11.0	38.0
34-35	32.37366191989415	37.5	31.0	38.0	11.0	38.0
36-37	30.490724271444527	36.0	25.0	38.0	11.0	38.0
38-39	28.8900578072297	34.0	22.0	37.5	11.0	38.0
40-41	29.898175764346064	35.0	24.5	37.5	11.0	38.0
42-43	30.88318167521664	36.5	25.5	38.0	11.0	38.0
44-45	32.466652386581785	37.0	31.0	38.0	11.0	38.0
46-47	31.128729542422697	36.5	27.5	38.0	11.0	38.0
48-49	30.55877791395074	35.5	25.0	38.0	11.0	38.0
50-51	28.485713588494953	34.0	21.0	37.5	11.0	38.0
52-53	31.5835384965782	36.5	28.5	38.0	11.0	38.0
54-55	30.735531924123947	36.5	26.0	38.0	11.0	38.0
56-57	31.135977137403337	36.5	27.0	38.0	11.0	38.0
58-59	32.12651161025129	37.0	31.0	38.0	11.0	38.0
60-61	32.62202422914656	38.0	32.0	38.0	11.0	38.0
62-63	32.50385020010195	38.0	32.0	38.0	11.0	38.0
64-65	32.2098575968244	37.5	30.0	38.0	11.0	38.0
66-67	30.30096634599742	36.0	24.0	38.0	11.0	38.0
68-69	30.397614502781906	36.0	24.5	38.0	11.0	38.0
70-71	30.01473650531978	35.0	24.0	38.0	11.0	38.0
72-73	28.872680390009002	34.0	22.0	37.5	11.0	38.0
74-75	30.763134062882983	36.0	25.5	38.0	11.0	38.0
76	30.959084845395488	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	0.0
5	2.0
6	0.0
7	2.0
8	2.0
9	0.0
10	2.0
11	13.0
12	59.0
13	349.0
14	996.0
15	1777.0
16	2754.0
17	4418.0
18	5838.0
19	6042.0
20	5496.0
21	4173.0
22	3427.0
23	2917.0
24	2793.0
25	2828.0
26	3012.0
27	3542.0
28	4220.0
29	5117.0
30	6140.0
31	7763.0
32	10097.0
33	13459.0
34	18321.0
35	24528.0
36	28275.0
37	16040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.71833888268277	17.353014543995315	11.07339240588701	31.8552541674349
2	20.7395637885969	16.909970391418934	38.183681658948196	24.166784161035977
3	19.93709498115561	16.51310973129796	36.024511265963504	27.52528402158293
4	14.73658523358911	18.047232992597813	31.92267020959301	35.29351156422006
5	14.607442274112556	27.99312386798694	39.07681962625945	18.32261423164105
6	27.985531924123947	31.95611856447187	17.122544819582878	22.935804691821307
7	28.17641508410789	24.426537097491405	19.84967951151264	27.547368306888064
8	23.72004490214261	31.06436516070954	18.53135286684996	26.684237070297883
9	23.044176184123472	15.219796310155207	19.772991030271477	41.96303647544985
10-11	25.275154354882005	22.35047845268213	26.84703885377434	25.527328338661526
12-13	24.372605325025017	18.367512954632346	28.87520438627175	28.384677334070886
14-15	24.324903267091287	21.344960262907435	23.26524746542154	31.064889004579733
16-17	25.77802362881555	21.439204744201938	25.48923904584489	27.29353258113762
18-19	22.836573659806394	21.2999267876027	27.31798584560319	28.54551370698772
20-21	24.827275783902213	21.344320437314938	26.671113569561495	27.157290209221358
22-23	26.088418403869934	20.38113625024404	26.382351027137247	27.14809431874878
24-25	23.49510298376338	23.673250252171933	22.99401295024892	29.837633813815767
26-27	24.064066723969166	22.84254737624152	26.50927459917625	26.584111300613067
28-29	23.859596794967395	23.598747271519407	25.27230575251834	27.269350180994863
30-31	23.724341766316876	23.011469942243554	25.637896906098323	27.62629138534125
32-33	23.825160319418647	22.97238303122331	25.463468864816498	27.738987784541546
34-35	23.98413223499043	23.105602524959462	25.574976003123663	27.335289236926446
36-37	25.045486714625437	20.52393102831158	29.561619245693336	24.868963011369644
38-39	24.6157684089504	19.517446337733332	32.94874019762031	22.918045055695956
40-41	24.145383157398093	22.21285226591755	25.248175796047086	28.39358878063727
42-43	21.42202506521185	22.592584558484592	26.79108888876838	29.194301487535178
44-45	21.130125192859914	21.125244379607963	29.258306195107796	28.486324232424327
46-47	23.5636251230365	20.297565300859834	29.37311716870982	26.76569240739385
48-49	23.31971224894317	20.76786038704849	29.839665284673433	26.07276207933491
50-51	25.6026399561815	19.62911544114058	30.920513891223827	23.84773071145409
52-53	19.946473167438114	22.55692310195316	29.30011090412623	28.196492826482494
54-55	22.32366562903348	21.370272351594956	28.35856915084005	27.947492868531516
56-57	22.41007174737116	22.120479183500816	27.413949250801263	28.055499818326762
58-59	20.17294582766157	22.991653696181523	28.057464220442206	28.7779362557147
60-61	19.942676938178405	22.30501617464824	28.017798892043377	29.734507995129984
62-63	20.07142314825862	22.94922828291593	27.594173346204325	29.385175222621125
64-65	20.816985045892704	23.461814829376753	26.439349774264837	29.281850350465703
66-67	19.575256177685223	24.94664942093946	22.662946361992155	32.81514803938317
68-69	20.150747499294813	24.925412806214332	22.875485494825003	32.04835419966585
70-71	19.835947774448528	25.720948520140464	21.804303320362813	32.6388003850482
72-73	19.23908121402095	25.654639077417833	21.104907657724837	34.00137205083638
74-75	21.03597691923903	25.10303911147747	23.14149981561422	30.71948415366928
76	22.21945042489845	24.286729176721963	23.525328503175214	29.96849189520437
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	1.0
4	2.0
5	1.5
6	1.5
7	1.5
8	1.0
9	1.5
10	2.0
11	1.0
12	0.0
13	1.5
14	1.5
15	1.0
16	2.5
17	3.0
18	8.0
19	19.0
20	33.0
21	41.0
22	60.0
23	96.5
24	152.5
25	191.0
26	236.0
27	371.5
28	565.0
29	668.0
30	822.5
31	1164.0
32	1617.5
33	1884.0
34	2209.5
35	2911.0
36	3778.5
37	4270.0
38	4697.0
39	5672.0
40	6752.5
41	7845.5
42	8406.0
43	8697.5
44	9247.0
45	9748.5
46	9992.0
47	9978.0
48	9690.0
49	9075.0
50	8734.0
51	8421.0
52	7665.0
53	6871.0
54	6520.0
55	6227.0
56	5651.5
57	5179.0
58	4989.0
59	4866.5
60	4615.5
61	4378.5
62	4270.0
63	4319.0
64	4112.5
65	3722.5
66	3458.0
67	3328.0
68	3181.0
69	2872.5
70	2565.0
71	2419.0
72	2286.0
73	1981.5
74	1682.0
75	1554.0
76	1361.5
77	1037.5
78	786.0
79	666.0
80	595.0
81	429.5
82	283.0
83	231.0
84	179.0
85	103.5
86	54.0
87	28.0
88	25.0
89	17.0
90	7.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	5.422817044998537E-4
4	5.422817044998537E-4
5	0.0
6	0.0
7	0.0
8	0.002711408522499268
9	0.004338253635998829
10-11	0.005151676192748609
12-13	0.006778521306248169
14-15	0.0035248310792490483
16-17	0.006778521306248169
18-19	0.00596509874949839
20-21	0.004338253635998829
22-23	0.005422817044998536
24-25	0.004338253635998829
26-27	0.002440267670249341
28-29	0.004609394488248755
30-31	0.00596509874949839
32-33	0.004609394488248755
34-35	0.0037959719314989747
36-37	0.006236239601748316
38-39	0.006507380453998243
40-41	0.0056939578972484625
42-43	0.0037959719314989747
44-45	0.0056939578972484625
46-47	0.005151676192748609
48-49	0.0056939578972484625
50-51	0.004880535340498682
52-53	0.006778521306248169
54-55	0.006507380453998243
56-57	0.004880535340498682
58-59	0.0070496621584980965
60-61	0.006778521306248169
62-63	0.006507380453998243
64-65	0.004609394488248755
66-67	0.006778521306248169
68-69	0.031452338860991504
70-71	0.007320803010748024
72-73	0.0056939578972484625
74-75	0.005422817044998536
76	0.004880535340498682
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	184406.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.44272187931145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.00655237333257	85.96215023507577
2	4.260815796366237	7.7924118769218955
3	0.9982510212986391	2.7384837151905015
4	0.348057368524353	1.2730925259206967
5	0.1863965737275211	0.8522305025311143
6	0.078845887842619	0.4325929555991996
7	0.049695644906007135	0.3181013525027122
8	0.0307550410430312	0.22498597315877686
9	0.015860787103725252	0.13053181895216265
>10	0.024769505855300075	0.2754190441471947
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0010845634089997071	0.0
2	0.0	0.0	0.0	0.0010845634089997071	0.0
3	0.0	0.0	0.0	0.0016268451134995607	0.0
4	0.0	0.0	0.0	0.0021691268179994143	0.0
5	0.0	0.0	0.0	0.002711408522499268	0.0
6	0.0	0.0	0.0	0.002711408522499268	0.0
7	0.0	0.0	0.0	0.002711408522499268	0.0
8	0.0	0.0	0.0	0.002711408522499268	0.0
9	0.0	0.0	0.0	0.0070496621584980965	0.0
10	0.0	0.0	0.0	0.009761070680997365	0.0
11	0.0	0.0	0.0	0.009761070680997365	0.0
12	0.0	0.0	0.0	0.010845634089997072	0.0
13	0.0	0.0	0.0	0.011387915794496925	0.0
14	0.0	0.0	0.0	0.01355704261249634	0.0
15	0.0	0.0	0.0	0.014641606021496046	0.0
16	0.0	0.0	0.0	0.014641606021496046	0.0
17	0.0	0.0	0.0	0.0151838877259959	0.0
18	0.0	0.0	0.0	0.0151838877259959	0.0
19	0.0	0.0	0.0	0.018979859657494876	0.0
20	0.0	0.0	0.0	0.020064423066494582	0.0
21	0.0	0.0	0.0	0.020064423066494582	0.0
22	0.0	0.0	0.0	0.026571803520492824	0.0
23	0.0	0.0	0.0	0.03199462056549136	0.0
24	0.0	0.0	0.0	0.034163747383490775	0.0
25	0.0	0.0	0.0	0.03687515590599005	0.0
26	0.0	0.0	0.0	0.040128846132989164	0.0
27	0.0	0.0	0.0	0.08025769226597833	0.0
28	0.0	0.0	0.0	0.1252670737394662	0.0
29	0.0	0.0	0.0	0.16756504669045474	0.0
30	0.0	0.0	0.0	0.21094758305044303	0.0
31	0.0	0.0	0.0	0.2396885133889353	0.0
32	0.0	0.0	0.0	0.2987972191794193	0.0
33	0.0	0.0	0.0	0.36061733349240266	0.0
34	0.0	5.422817044998536E-4	0.0	0.42840254655488436	0.0
35	0.0	5.422817044998536E-4	0.0	0.6062709456308363	0.0
36	0.0	5.422817044998536E-4	0.0	0.7364185547108012	0.0
37	0.0	5.422817044998536E-4	0.0	1.14855265013069	0.0
38	0.0	5.422817044998536E-4	0.0	1.5297766883940869	0.0
39	0.0	5.422817044998536E-4	0.0	2.0015617713089595	0.0
40	0.0	5.422817044998536E-4	0.0	2.5422166306953136	0.0
41	0.0	5.422817044998536E-4	0.0	3.162586900643146	0.0
42	0.0	5.422817044998536E-4	0.0	3.8306779605869656	0.0
43	0.0	5.422817044998536E-4	0.0	4.497142175417285	0.0
44	0.0	5.422817044998536E-4	0.0	5.240068110582085	0.0
45	0.0	5.422817044998536E-4	0.0	5.745474659175949	0.0
46	0.0	5.422817044998536E-4	0.0	6.37289459128228	0.0
47	0.0	5.422817044998536E-4	0.0	7.077318525427589	0.0
48	0.0	5.422817044998536E-4	0.0	7.5539841436829605	0.0
49	0.0	5.422817044998536E-4	0.0	8.03824170580133	0.0
50	0.0	5.422817044998536E-4	0.0	8.678676398815657	0.0
51	0.0	5.422817044998536E-4	0.0	9.36954329034847	0.0
52	0.0	5.422817044998536E-4	0.0	9.975814235979307	0.0
53	0.0	5.422817044998536E-4	0.0	10.742058284437599	0.0
54	0.0	5.422817044998536E-4	0.0	11.51372514994089	0.0
55	0.0	5.422817044998536E-4	0.0	12.029435050920252	0.0
56	0.0	5.422817044998536E-4	0.0	12.569005346897606	0.0
57	0.0	5.422817044998536E-4	0.0	12.988731386180493	0.0
58	0.0	5.422817044998536E-4	0.0	13.382427903647386	0.0
59	0.0	5.422817044998536E-4	0.0	13.81625326724727	0.0
60	0.0	5.422817044998536E-4	0.0	14.098239753587194	0.0
61	0.0	5.422817044998536E-4	0.0	14.41005173367461	0.0
62	0.0	5.422817044998536E-4	0.0	14.616118781384554	0.0
63	0.0	5.422817044998536E-4	0.0	14.945283776015964	0.0
64	0.0	5.422817044998536E-4	0.0	15.228897107469388	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACGAG	15	0.0022231305	69.99051	19
TCTGGAC	15	0.0022231305	69.99051	3
TCGGTTC	20	0.0069391048	52.507122	52
TACCCAG	20	0.006946553	52.492886	5
ACGTGTA	20	0.006946553	52.492886	34
CAGTGTA	40	7.4567834E-7	52.492886	34
GATTCGA	20	0.006946553	52.492886	23
CGACTAA	55	2.2719178E-9	50.90219	22
ATCTCGG	155	0.0	47.412926	42
GCCGTAC	30	5.862578E-4	46.672993	54
CGTGTAT	45	1.6796148E-6	46.660336	35
ACGCTTC	125	0.0	44.793926	15
GACGCTT	95	0.0	44.20453	14
TCTCGGT	200	0.0	43.744072	43
AGATCTC	145	0.0	43.442387	40
GTGTCGC	65	9.958967E-9	43.071083	36
GTAGATC	125	0.0	41.994305	38
CCGACTA	110	0.0	41.358032	21
CGGTGGT	215	0.0	40.692154	46
CGCCGTA	95	3.6379788E-12	40.53181	53
>>END_MODULE