FastQCFastQC Report
Wed 25 May 2016
SRR1779767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184406
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT2400113.015303189700985TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG28431.5417068858930838TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC15980.866566163790766RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT8650.46907367439237335TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC7020.3806817565588972RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC5620.3047623179289177TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT4560.2472804572519332No Hit
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC3660.19847510384694642RNA PCR Primer, Index 5 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG2670.14478921510146092TruSeq Adapter, Index 5 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC2000.10845634089997071No Hit
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC1940.10520265067297159TruSeq Adapter, Index 5 (95% over 24bp)
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1860.10086439703697278TruSeq Adapter, Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGT150.002221935270.032
AACTTGG150.002221935270.019
GTTATCT150.002221935270.034
CACGACA200.006942829652.50000422
AGAACTT200.006942829652.50000445
TTGCGCA200.006942829652.50000428
TCTCGTA33850.050.768138
CTCGTAT35100.050.55555339
TCGTATG35350.050.39603840
CTTGAAA34950.050.3719657
CGTATGC35500.050.28169341
ATCTCGT34200.050.2485437
TATGCCG35400.050.22598643
TTGAAAA35150.050.1849258
TGATCTC33900.050.17699435
TGAAAAA35150.050.0853559
ATGCCGT35500.050.0845144
CCGTCTT35100.050.05697647
GTATGCC35600.050.04213342
CGTCTTC35250.049.74468248
Produced by FastQC (version 0.11.5)