##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779767_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	184406
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.67831849289069	34.0	34.0	34.0	34.0	34.0
2	33.67169723327874	34.0	34.0	34.0	34.0	34.0
3	33.69366506512803	34.0	34.0	34.0	34.0	34.0
4	33.747486524299646	34.0	34.0	34.0	34.0	34.0
5	33.74963395984946	34.0	34.0	34.0	34.0	34.0
6	37.43073978070128	38.0	38.0	38.0	37.0	38.0
7	37.4160764834116	38.0	38.0	38.0	37.0	38.0
8	37.315076515948505	38.0	38.0	38.0	36.0	38.0
9	37.319474420572	38.0	38.0	38.0	37.0	38.0
10-11	37.2587253126254	38.0	38.0	38.0	36.0	38.0
12-13	37.22636194050085	38.0	38.0	38.0	36.0	38.0
14-15	37.24494051169702	38.0	38.0	38.0	36.0	38.0
16-17	37.22153834473933	38.0	38.0	38.0	36.0	38.0
18-19	37.201647451818275	38.0	38.0	38.0	36.0	38.0
20-21	37.22686897389456	38.0	38.0	38.0	36.0	38.0
22-23	37.18641747014739	38.0	38.0	38.0	36.0	38.0
24-25	37.1491328915545	38.0	38.0	38.0	36.0	38.0
26-27	37.07587876750214	38.0	38.0	38.0	36.0	38.0
28-29	37.068088890817	38.0	38.0	38.0	36.0	38.0
30-31	36.915149181696904	38.0	38.0	38.0	35.0	38.0
32-33	36.85149615522272	38.0	38.0	38.0	35.0	38.0
34-35	36.825211761005605	38.0	37.5	38.0	35.0	38.0
36-37	36.94102957604416	38.0	38.0	38.0	35.0	38.0
38-39	36.2384304198345	38.0	37.0	38.0	34.0	38.0
40-41	36.786229840677635	38.0	37.0	38.0	34.5	38.0
42-43	37.00697916553692	38.0	37.5	38.0	36.0	38.0
44-45	37.01665618255372	38.0	38.0	38.0	36.0	38.0
46-47	37.05711310911792	38.0	38.0	38.0	36.0	38.0
48-49	37.02928321204299	38.0	38.0	38.0	36.0	38.0
50-51	36.9647272865308	38.0	38.0	38.0	35.0	38.0
52-53	36.951623049141574	38.0	38.0	38.0	35.5	38.0
54-55	36.95759899352516	38.0	38.0	38.0	36.0	38.0
56-57	36.98559699792848	38.0	38.0	38.0	36.0	38.0
58-59	36.91891261672613	38.0	38.0	38.0	35.5	38.0
60-61	36.94711126536013	38.0	38.0	38.0	35.5	38.0
62-63	36.96795386267258	38.0	38.0	38.0	36.0	38.0
64-65	36.98983764085767	38.0	38.0	38.0	36.0	38.0
66-67	36.93518106786114	38.0	38.0	38.0	35.5	38.0
68-69	36.75545264253874	38.0	38.0	38.0	35.0	38.0
70-71	35.582849798813484	38.0	36.5	38.0	28.5	38.0
72-73	33.61912844484453	38.0	35.5	38.0	20.0	38.0
74-75	33.376601086732535	38.0	35.0	38.0	11.0	38.0
76	32.99073782848714	38.0	34.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	6.0
22	14.0
23	19.0
24	51.0
25	94.0
26	172.0
27	311.0
28	540.0
29	914.0
30	1484.0
31	2419.0
32	3651.0
33	5901.0
34	10316.0
35	22758.0
36	29031.0
37	106722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.346973525807186	15.088988427708424	10.916130711582053	37.647907334902335
2	19.032460982831363	15.690378837998765	40.92328883008145	24.353871349088426
3	17.986399574851145	17.523290999208267	38.153313883496196	26.33699554244439
4	13.580360725789834	26.48124247584135	30.545101569363254	29.393295229005563
5	14.013101525980717	29.129204038914136	41.90698784204419	14.950706593060964
6	27.928592345151458	42.07238376191664	15.239200459854885	14.759823433077015
7	25.81748966953353	24.800169191891804	17.55420105636476	31.828140082209906
8	22.865850351940825	41.33216923527434	15.26414541826188	20.537834994522957
9	20.986844245848836	15.325423250870362	16.089498172510655	47.598234330770154
10-11	23.568376300120384	26.963873192846222	26.922388642451985	22.545361864581412
12-13	22.715909460646618	17.712547314078716	28.266433847054866	31.305109378219797
14-15	21.421754172857714	17.747795624871205	19.15474550719608	41.675704695075
16-17	29.763402492326712	27.036810082101447	18.501024912421503	24.69876251315033
18-19	29.840135353513443	21.418229341778467	19.355118597008776	29.386516707699318
20-21	21.692623884255394	20.0015183887726	19.320412567920783	38.985445159051224
22-23	22.194505601770008	27.654740084379032	19.212769649577563	30.9379846642734
24-25	29.877823931976184	27.709510536533514	18.92807175471514	23.48459377677516
26-27	29.499040161383032	28.8911423706387	19.425615218593755	22.184202249384512
28-29	21.96023990542607	28.99986985239092	19.867303666908885	29.172586575274124
30-31	21.33146426905849	29.161198659479627	19.48526620608874	30.022070865373145
32-33	21.21324685747752	36.73118011344533	19.9874190644556	22.068153964621544
34-35	29.47951802002104	22.06842510547379	27.506968319902825	20.945088554602346
36-37	30.10476882530937	26.390139149485375	21.81301042265436	21.692081602550893
38-39	21.59718230426342	21.29377569059575	28.404173399997827	28.704868605143
40-41	20.810060410181883	18.576673210199235	29.902226608678678	30.711039770940207
42-43	28.17749964751689	18.80660065290717	29.50934351376853	23.506556185807405
44-45	20.48279340151622	26.532216956064335	30.664674685205473	22.320314957213974
46-47	27.665585718469032	19.119226055551337	23.929807056169537	29.285381169810094
48-49	26.69110549548279	18.639306747068968	22.74275240501936	31.926835352428878
50-51	20.123260631432814	18.861371105061657	30.741678687244452	30.273689576261077
52-53	18.14230556489485	19.04032406754661	38.70101840504105	24.116351962517488
54-55	17.654794312549484	19.026224743229612	32.360118434324264	30.95886250989664
56-57	25.45633005433663	19.215752198952313	24.358480743576674	30.969437003134388
58-59	18.612463802696226	19.716007071353427	36.82011431298331	24.85141481296704
60-61	25.70008568050931	27.28897107469388	23.44229580382417	23.568647440972637
62-63	17.829951303102938	37.395746342309906	20.59314772838194	24.18115462620522
64-65	17.663199678969228	38.59662917692483	19.398772274221013	24.341398869884927
66-67	17.938949925707405	38.58469897942583	18.85215231608516	24.6241987787816
68-69	17.824257345205687	38.97839549689272	18.69245035410995	24.504896803791635
70-71	18.168335086710844	37.73602811188356	18.845102653926663	25.25053414747893
72-73	18.387688036181036	33.96608570220058	19.73091981822717	27.915306443391213
74-75	18.792501328590173	32.85467934882813	20.25015455028578	28.10266477229591
76	19.11271867509734	33.56235697320044	19.936444584232618	27.388479767469605
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.0
15	2.0
16	5.5
17	7.0
18	12.5
19	22.0
20	43.5
21	61.0
22	74.5
23	109.5
24	185.5
25	240.0
26	302.5
27	448.5
28	586.5
29	641.0
30	814.5
31	1071.5
32	1421.0
33	1687.0
34	1897.0
35	2354.5
36	3032.0
37	3462.0
38	3913.0
39	9804.0
40	15945.0
41	15285.0
42	13924.0
43	12486.0
44	10243.5
45	8763.5
46	8088.0
47	7993.0
48	7493.5
49	6838.5
50	6588.0
51	6310.5
52	5510.5
53	4771.5
54	4555.0
55	4379.0
56	4000.0
57	3708.0
58	3619.0
59	3545.5
60	3303.0
61	3261.0
62	3388.0
63	3360.0
64	3331.5
65	3205.5
66	3017.5
67	2955.0
68	2942.5
69	2834.5
70	2616.0
71	2493.0
72	2386.0
73	2164.0
74	1900.0
75	1751.0
76	1633.0
77	1378.0
78	1111.5
79	982.0
80	891.5
81	651.5
82	418.0
83	334.0
84	278.5
85	164.0
86	88.0
87	71.0
88	48.5
89	19.0
90	12.5
91	8.5
92	4.0
93	4.5
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	184406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.87633808010585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.612774131583166	15.790700953331235
2	24.62782135425004	16.686008047460497
3	12.955018408836242	13.166057503551945
4	6.236593564911157	8.450918082925718
5	3.1102929406114934	5.268266759216077
6	1.700016007683688	3.455419021073067
7	1.043700976468705	2.474973699337332
8	0.7139426924923964	1.9348611216554776
9	0.5314550984472547	1.6203377330455626
>10	2.4267648471266208	12.937756905957507
>50	0.01440691531935329	0.3307918397449107
>100	0.017608452056987356	1.3058143444356474
>500	0.004802305106451097	1.1545177488801883
>1k	0.003201536737634064	2.40827304968385
>5k	0.0	0.0
>10k+	0.001600768368817032	13.015303189700985
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	24001	13.015303189700985	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	2843	1.5417068858930838	TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	1598	0.866566163790766	RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	865	0.46907367439237335	TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	702	0.3806817565588972	RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	562	0.3047623179289177	TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT	456	0.2472804572519332	No Hit
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	366	0.19847510384694642	RNA PCR Primer, Index 5 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	267	0.14478921510146092	TruSeq Adapter, Index 5 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	200	0.10845634089997071	No Hit
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	194	0.10520265067297159	TruSeq Adapter, Index 5 (95% over 24bp)
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	186	0.10086439703697278	TruSeq Adapter, Index 5 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0016268451134995607	0.0
2	0.0	0.0	0.0	0.0016268451134995607	0.0
3	0.0	0.0	0.0	0.0021691268179994143	0.0
4	0.0	0.0	0.0	0.0032536902269991214	0.0
5	0.0	0.0	0.0	0.0043382536359988285	0.0
6	0.0	0.0	0.0	0.0043382536359988285	0.0
7	0.0	0.0	0.0	0.0043382536359988285	0.0
8	0.0	0.0	0.0	0.0043382536359988285	0.0
9	0.0	0.0	0.0	0.01193019749899678	0.0
10	0.0	0.0	0.0	0.01681073283949546	0.0
11	0.0	0.0	0.0	0.01681073283949546	0.0
12	0.0	0.0	0.0	0.017895296248495167	0.0
13	0.0	0.0	0.0	0.018437577952995023	0.0
14	0.0	0.0	0.0	0.018437577952995023	0.0
15	0.0	0.0	0.0	0.020606704770994435	0.0
16	0.0	0.0	0.0	0.020606704770994435	0.0
17	0.0	0.0	0.0	0.021691268179994144	0.0
18	0.0	0.0	0.0	0.02277583158899385	0.0
19	0.0	0.0	0.0	0.02711408522499268	0.0
20	0.0	0.0	0.0	0.0303677754519918	0.0
21	0.0	0.0	0.0	0.0303677754519918	0.0
22	0.0	0.0	0.0	0.03470602908799063	0.0
23	0.0	0.0	0.0	0.038502001019489605	0.0
24	0.0	0.0	0.0	0.03958656442848931	0.0
25	0.0	0.0	0.0	0.04121340954198887	0.0
26	0.0	0.0	0.0	0.04500938147348785	0.0
27	0.0	0.0	0.0	0.09598386169647409	0.0
28	0.0	0.0	0.0	0.1507543138509593	0.0
29	0.0	0.0	0.0	0.22233549884493997	0.0
30	0.0	0.0	0.0	0.28415561315792326	0.0
31	0.0	0.0	0.0	0.37580122121839854	0.0
32	0.0	0.0	0.0	0.48425756211836923	0.0
33	0.0	0.0	0.0	0.6078977907443359	0.0
34	0.0	0.0	0.0	0.7765474008437904	0.0
35	0.0	0.0	0.0	1.0520265067297159	0.0
36	0.0	0.0	0.0	1.4522304046506078	0.0
37	0.0	0.0	0.0	2.0742275197119397	0.0
38	0.0	0.0	0.0	2.728219255338763	0.0
39	0.0	0.0	0.0	3.4619264015270654	0.0
40	0.0	0.0	0.0	4.244438901120354	0.0
41	0.0	0.0	0.0	5.077925880936629	0.0
42	0.0	0.0	0.0	6.025834300402373	0.0
43	0.0	0.0	0.0	6.929817901803629	0.0
44	0.0	0.0	0.0	7.933581336832858	0.0
45	0.0	0.0	0.0	8.92921054629459	0.0
46	0.0	0.0	0.0	9.916163248484322	0.0
47	0.0	0.0	0.0	10.882509245903062	0.0
48	0.0	0.0	0.0	11.781070030259318	0.0
49	0.0	0.0	0.0	12.694814702341572	0.0
50	0.0	0.0	0.0	13.490341962842857	0.0
51	0.0	0.0	0.0	14.307018209819637	0.0
52	0.0	0.0	0.0	15.164365584633906	0.0
53	0.0	0.0	0.0	16.00381766319968	0.0
54	0.0	0.0	0.0	16.870926108694945	0.0
55	0.0	0.0	0.0	17.711462750669718	0.0
56	0.0	0.0	0.0	18.48963699662701	0.0
57	0.0	0.0	0.0	19.18267301497782	0.0
58	0.0	0.0	0.0	19.86160970901164	0.0
59	0.0	0.0	0.0	20.491198767935966	0.0
60	0.0	0.0	0.0	21.1234992353828	0.0
61	0.0	0.0	0.0	21.799724520894113	0.0
62	0.0	0.0	0.0	22.423348481068945	0.0
63	0.0	0.0	0.0	23.10933483726126	0.0
64	0.0	0.0	0.0	23.764411136297085	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAGT	15	0.0022219352	70.0	32
AACTTGG	15	0.0022219352	70.0	19
GTTATCT	15	0.0022219352	70.0	34
CACGACA	20	0.0069428296	52.500004	22
AGAACTT	20	0.0069428296	52.500004	45
TTGCGCA	20	0.0069428296	52.500004	28
TCTCGTA	3385	0.0	50.7681	38
CTCGTAT	3510	0.0	50.555553	39
TCGTATG	3535	0.0	50.396038	40
CTTGAAA	3495	0.0	50.37196	57
CGTATGC	3550	0.0	50.281693	41
ATCTCGT	3420	0.0	50.24854	37
TATGCCG	3540	0.0	50.225986	43
TTGAAAA	3515	0.0	50.18492	58
TGATCTC	3390	0.0	50.176994	35
TGAAAAA	3515	0.0	50.08535	59
ATGCCGT	3550	0.0	50.08451	44
CCGTCTT	3510	0.0	50.056976	47
GTATGCC	3560	0.0	50.042133	42
CGTCTTC	3525	0.0	49.744682	48
>>END_MODULE