FastQCFastQC Report
Wed 25 May 2016
SRR1779766_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779766_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34305
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG150.002206497670.042
AGATCTC309.949508E-870.040
GTTCGCC207.819644E-570.050
TCTCGGT207.819644E-570.043
CGTGTAT207.819644E-570.035
ACATCTT150.002206497670.08
ATCTCTG252.3583703E-455.99999642
TGGTTGC252.3583703E-455.99999649
TATCTCT252.3583703E-455.99999641
GCCGTAT252.3583703E-455.99999654
TATCATT200.006894962452.558
GTGTTCG200.006894962452.548
GTAGATC200.006894962452.538
TCTGTGG200.006894962452.545
TATTATC200.006894962452.534
GTGTATA407.271774E-752.536
CGGTGGT407.271774E-752.546
TAGATCT407.271774E-752.539
CGCAACC200.006894962452.540
AAGTGTA200.006894962452.534
Produced by FastQC (version 0.11.5)