##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779766_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34305
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.071738813584027	33.0	23.0	34.0	12.0	34.0
2	26.988689695379684	32.0	23.0	34.0	12.0	34.0
3	26.237574697565954	32.0	21.0	34.0	11.0	34.0
4	28.00431423990672	33.0	23.0	34.0	12.0	34.0
5	27.69733275032794	33.0	23.0	34.0	11.0	34.0
6	29.84392945634747	36.0	23.0	38.0	11.0	38.0
7	30.890803089928582	36.0	26.0	38.0	12.0	38.0
8	30.181314677160763	36.0	25.0	38.0	11.0	38.0
9	31.630287130155953	37.0	27.0	38.0	12.0	38.0
10-11	31.111397755429238	37.0	26.0	38.0	11.0	38.0
12-13	30.408599329543797	35.5	24.5	38.0	11.0	38.0
14-15	30.211849584608657	35.0	24.5	38.0	11.0	38.0
16-17	30.031555166885294	35.5	24.0	38.0	11.0	38.0
18-19	30.46449497157849	36.0	25.0	38.0	11.0	38.0
20-21	29.530607783121994	35.0	23.5	38.0	11.0	38.0
22-23	29.55593936743915	35.5	23.5	38.0	11.0	38.0
24-25	30.59245008016324	36.5	24.5	38.0	11.0	38.0
26-27	29.137662148374872	35.0	22.0	38.0	11.0	38.0
28-29	31.012971869989798	37.0	26.0	38.0	11.0	38.0
30-31	31.51257834134966	37.0	26.5	38.0	11.0	38.0
32-33	31.64241364232619	37.0	27.0	38.0	11.0	38.0
34-35	31.682130884710684	38.0	27.0	38.0	11.0	38.0
36-37	29.873648156245444	35.0	24.0	38.0	11.0	38.0
38-39	28.38944760239032	34.0	21.0	37.5	11.0	38.0
40-41	29.24703396006413	34.0	22.0	37.5	11.0	38.0
42-43	30.24910362920857	35.5	24.5	38.0	11.0	38.0
44-45	31.68902492348054	37.0	27.0	38.0	11.0	38.0
46-47	30.41001311762134	36.0	24.5	38.0	11.0	38.0
48-49	29.65091094592625	35.5	23.5	38.0	11.0	38.0
50-51	27.863853665646406	33.0	21.0	37.5	11.0	38.0
52-53	30.782947092260603	36.5	25.5	38.0	11.0	38.0
54-55	30.055268911237427	35.5	24.0	38.0	11.0	38.0
56-57	30.41826264392946	36.0	24.5	38.0	11.0	38.0
58-59	31.363620463489287	37.0	26.5	38.0	11.0	38.0
60-61	31.861434193266287	38.0	27.0	38.0	11.0	38.0
62-63	31.775134819997085	38.0	27.5	38.0	11.0	38.0
64-65	31.48517708788806	37.5	27.0	38.0	11.0	38.0
66-67	29.850794344847692	36.0	23.5	38.0	11.0	38.0
68-69	29.740139921294272	35.5	23.5	38.0	11.0	38.0
70-71	29.289287275907302	35.0	22.0	38.0	11.0	38.0
72-73	28.277029587523685	34.0	21.0	37.5	11.0	38.0
74-75	29.90212796968372	35.5	23.5	38.0	11.0	38.0
76	30.355079434484768	36.0	24.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	19.0
13	107.0
14	305.0
15	507.0
16	783.0
17	1141.0
18	1365.0
19	1394.0
20	1218.0
21	889.0
22	686.0
23	511.0
24	466.0
25	493.0
26	450.0
27	499.0
28	584.0
29	730.0
30	908.0
31	1184.0
32	1692.0
33	2404.0
34	3408.0
35	4664.0
36	5283.0
37	2613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.28640139921294	18.038186853228392	13.86095321381723	32.81445853374144
2	20.282757615507943	15.432152747412914	41.4954088325317	22.78968080454744
3	18.571636787640287	17.14327357528057	38.72030316280426	25.564786474274886
4	13.866783267745229	20.384783559247925	30.12971869989797	35.618714473108874
5	15.10275470048098	25.870864305494827	40.387698586211926	18.638682407812272
6	25.39279988339892	34.19909634164116	16.283340620900745	24.124763154059174
7	24.766069086139048	24.79521935577904	19.393674391488123	31.045037166593794
8	21.69654569304766	33.55196035563329	16.528202885876695	28.223291065442357
9	21.069814895787786	16.507797697128698	18.854394403148227	43.567993003935285
10-11	23.315843171549336	23.89301851042122	26.567555749890687	26.223582568138752
12-13	22.281008599329542	18.412767818102317	30.221542049263956	29.084681533304185
14-15	24.09269785745518	22.221250546567557	22.579798863139484	31.10625273283778
16-17	26.363503862410724	20.787057280279843	24.39877568867512	28.45066316863431
18-19	21.25200408103775	21.183500947383763	27.85162512753243	29.712869844046057
20-21	25.796531117912842	20.69523393091386	25.515231015886897	27.993003935286403
22-23	26.88675120244862	19.877568867512025	24.919108001749017	28.316571928290337
24-25	21.823349365981635	24.063547587815187	22.576883836175483	31.536219210027696
26-27	24.4978866054511	23.14677160763737	25.61725695962688	26.738084827284652
28-29	23.024340475149398	23.60588835446728	24.895787786037022	28.473983384346308
30-31	22.69494242821746	23.04620317737939	25.566243987756888	28.692610406646263
32-33	22.543361026089492	23.165719282903368	25.847544089782833	28.443375601224314
34-35	22.935432152747413	23.212359714327356	25.29223145314094	28.559976679784288
36-37	25.980177816644805	19.54671330709809	29.793033085556043	24.680075790701064
38-39	25.233930913860952	18.61827721906428	33.67584900160326	22.471942865471505
40-41	23.94111645532721	23.216732254773355	24.174318612447166	28.667832677452264
42-43	20.253607345867948	24.180148666375164	26.171112082786767	29.395131904970125
44-45	20.39498615362192	21.46042850896371	28.68532283923626	29.459262498178106
46-47	25.24850604868095	20.049555458387992	27.24675703250255	27.455181460428506
48-49	25.025506485934994	20.94884127678181	28.306369333916336	25.719282903366857
50-51	29.683719574406066	18.581839382014284	28.466695816936305	23.267745226643346
52-53	20.142836321235972	22.367001894767526	28.232036146334355	29.258125637662147
54-55	23.80411018801924	21.020259437399798	26.573385803818685	28.60224457076228
56-57	24.19472380119516	21.454598455035708	25.96851770878881	28.382160034980323
58-59	20.7071958489411	23.231645993965806	27.355011733154544	28.70614642393855
60-61	20.217169508817957	22.059466550065586	27.428946217752515	30.294417723363942
62-63	20.094738376329982	23.11324879755138	27.019384929310597	29.772627896808046
64-65	20.583005392799883	24.212213962979156	26.268765486080746	28.93601515814021
66-67	19.226060341058155	27.054365252878586	22.127969683719577	31.591604722343682
68-69	19.415126246428365	26.919937022566913	22.291387252901043	31.37354947810368
70-71	19.69829470922606	27.60676286255648	21.307389593353737	31.387552834863726
72-73	19.89505902929602	27.923043288150417	20.577175338871882	31.60472234368168
74-75	21.40650051012972	25.44527036875091	23.40912403439732	29.739105086722052
76	23.127823932371374	23.10158868969538	24.296749744935138	29.473837632998105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	1.5
16	2.5
17	2.0
18	2.5
19	3.0
20	8.0
21	13.0
22	13.5
23	19.5
24	31.5
25	38.0
26	44.5
27	67.0
28	99.5
29	116.0
30	149.5
31	218.5
32	294.0
33	334.0
34	409.0
35	547.0
36	685.5
37	761.0
38	894.5
39	1114.5
40	1320.0
41	1535.0
42	1631.0
43	1706.5
44	1808.0
45	1856.0
46	1878.0
47	1922.0
48	1842.5
49	1660.0
50	1601.0
51	1538.5
52	1357.0
53	1199.0
54	1160.0
55	1064.0
56	892.0
57	775.5
58	735.0
59	719.5
60	681.5
61	643.5
62	628.0
63	641.0
64	682.5
65	693.0
66	673.0
67	671.0
68	671.0
69	644.0
70	601.0
71	585.0
72	573.5
73	487.5
74	400.0
75	387.0
76	335.5
77	252.5
78	184.5
79	148.0
80	137.0
81	95.0
82	51.0
83	38.0
84	26.0
85	12.5
86	8.5
87	6.0
88	5.0
89	2.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0014575134819997085
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.02040518874799592
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	34305.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.22183355196036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.4804867190978	96.72933974639265
2	1.3058317257753376	2.565223728319487
3	0.15135776821486868	0.44599912549191084
4	0.05045258940495623	0.19822183355196035
5	0.008903398130286393	0.043725404459991256
6	0.0029677993767621307	0.0174901617839965
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.002915026963999417	0.0
6	0.0	0.0	0.0	0.002915026963999417	0.0
7	0.0	0.0	0.0	0.002915026963999417	0.0
8	0.0	0.0	0.0	0.005830053927998834	0.0
9	0.0	0.0	0.0	0.00874508089199825	0.0
10	0.0	0.0	0.0	0.00874508089199825	0.0
11	0.0	0.0	0.0	0.014575134819997084	0.0
12	0.0	0.0	0.0	0.014575134819997084	0.0
13	0.0	0.0	0.0	0.014575134819997084	0.0
14	0.0	0.0	0.0	0.014575134819997084	0.0
15	0.0	0.0	0.0	0.014575134819997084	0.0
16	0.0	0.0	0.0	0.014575134819997084	0.0
17	0.0	0.0	0.0	0.014575134819997084	0.0
18	0.0	0.0	0.0	0.014575134819997084	0.0
19	0.0	0.0	0.0	0.0174901617839965	0.0
20	0.0	0.0	0.0	0.02040518874799592	0.0
21	0.0	0.0	0.0	0.026235242675994752	0.0
22	0.0	0.0	0.0	0.04664043142399067	0.0
23	0.0	0.0	0.0	0.052470485351989504	0.0
24	0.0	0.0	0.0	0.06121556624398776	0.0
25	0.0	0.0	0.0	0.06121556624398776	0.0
26	0.0	0.0	0.0	0.06121556624398776	0.0
27	0.0	0.0	0.0	0.07870572802798426	0.0
28	0.0	0.0	0.0	0.12534615945197494	0.0
29	0.0	0.0	0.0	0.1370062673079726	0.0
30	0.0	0.0	0.0	0.16324150998396736	0.0
31	0.0	0.0	0.0	0.18073167176796384	0.0
32	0.0	0.0	0.0	0.20113686051595978	0.0
33	0.0	0.0	0.0	0.22737210319195453	0.0
34	0.0	0.0	0.0	0.2565223728319487	0.0
35	0.0	0.0	0.0	0.32065296603993587	0.0
36	0.0	0.0	0.0	0.3731234513919254	0.0
37	0.0	0.0	0.0	0.5626002040518875	0.0
38	0.0	0.0	0.0	0.7316717679638537	0.0
39	0.0	0.0	0.0	0.9036583588398193	0.0
40	0.0	0.0	0.0	1.1135403002477773	0.0
41	0.0	0.0	0.0	1.3729777000437253	0.0
42	0.0	0.0	0.0	1.6936306660836613	0.0
43	0.0	0.0	0.0	1.9384929310596124	0.0
44	0.0	0.0	0.0	2.215420492639557	0.0
45	0.0	0.0	0.0	2.439877568867512	0.0
46	0.0	0.0	0.0	2.6963999416994606	0.0
47	0.0	0.0	0.0	3.0112228538113976	0.0
48	0.0	0.0	0.0	3.244425010931351	0.0
49	0.0	0.0	0.0	3.4688820871593062	0.0
50	0.0	0.0	0.0	3.7866200262352425	0.0
51	0.0	0.0	0.0	4.095612884419181	0.0
52	0.0	0.0	0.0	4.384200553855123	0.0
53	0.0	0.0	0.0	4.754408978283049	0.0
54	0.0	0.0	0.0	5.060486809502988	0.0
55	0.0	0.0	0.0	5.293688966622941	0.0
56	0.0	0.0	0.0	5.623087013554875	0.0
57	0.0	0.0	0.0	5.882524413350824	0.0
58	0.0	0.0	0.0	6.104066462614779	0.0
59	0.0	0.0	0.0	6.343098673662731	0.0
60	0.0	0.0	0.0	6.4975951027547	0.0
61	0.0	0.0	0.0	6.6491765048826705	0.0
62	0.0	0.0	0.0	6.7716076373706455	0.0
63	0.0	0.0	0.0	6.923189039498616	0.0
64	0.0	0.0	0.0	7.109750765194578	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGG	15	0.0022064976	70.0	42
AGATCTC	30	9.949508E-8	70.0	40
GTTCGCC	20	7.819644E-5	70.0	50
TCTCGGT	20	7.819644E-5	70.0	43
CGTGTAT	20	7.819644E-5	70.0	35
ACATCTT	15	0.0022064976	70.0	8
ATCTCTG	25	2.3583703E-4	55.999996	42
TGGTTGC	25	2.3583703E-4	55.999996	49
TATCTCT	25	2.3583703E-4	55.999996	41
GCCGTAT	25	2.3583703E-4	55.999996	54
TATCATT	20	0.0068949624	52.5	58
GTGTTCG	20	0.0068949624	52.5	48
GTAGATC	20	0.0068949624	52.5	38
TCTGTGG	20	0.0068949624	52.5	45
TATTATC	20	0.0068949624	52.5	34
GTGTATA	40	7.271774E-7	52.5	36
CGGTGGT	40	7.271774E-7	52.5	46
TAGATCT	40	7.271774E-7	52.5	39
CGCAACC	20	0.0068949624	52.5	40
AAGTGTA	20	0.0068949624	52.5	34
>>END_MODULE