FastQCFastQC Report
Wed 25 May 2016
SRR1779766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34305
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT408511.90788514793762No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT6641.9355779040956127RNA PCR Primer, Index 48 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG5481.5974347762716805No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC3491.0173444104357965No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC3170.9240635475878152No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2540.7404168488558519RNA PCR Primer, Index 48 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2080.6063256085118788No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC1250.3643783704999271No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT1230.3585483165719283RNA PCR Primer, Index 48 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1090.31773793907593645No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1060.3089928581839382No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1020.2973327503279405No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT800.23320215711995335No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT580.16907156391196618No Hit
TCTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT450.13117621337997376No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT420.1224311324879755No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTT150.002206497670.042
GTAATCG150.002206497670.036
CTAGTGG150.002206497670.04
CTATACA407.271774E-752.51
TTTCCTA200.006894962452.548
TTACAAA200.006894962452.529
CTTGAAA7200.044.23611557
AATCTCG6700.043.880636
TTGAAAA7200.043.75000458
CGTCTTC7300.043.63013548
GTCTTCT7300.043.63013549
TATGCCG7400.043.51351543
ATGCCGT7400.043.51351544
CTGCTTG7250.043.44827754
TCTTCTG7250.043.44827750
CCGTCTT7350.043.33333647
GCCGTCT7350.043.33333646
TCTCGTA6950.043.30935338
TGCCGTC7450.043.22147445
CTCGTAT7300.043.15068439