##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779766_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34305
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.34292377204489	34.0	34.0	34.0	33.0	34.0
2	33.37283194869553	34.0	34.0	34.0	33.0	34.0
3	33.367001894767526	34.0	34.0	34.0	34.0	34.0
4	33.44978866054511	34.0	34.0	34.0	34.0	34.0
5	33.39740562600204	34.0	34.0	34.0	34.0	34.0
6	36.783442646844485	38.0	37.0	38.0	34.0	38.0
7	36.791808774231164	38.0	37.0	38.0	34.0	38.0
8	36.55717825389885	38.0	37.0	38.0	34.0	38.0
9	36.62183355196036	38.0	37.0	38.0	34.0	38.0
10-11	36.486343098673665	38.0	37.0	38.0	34.0	38.0
12-13	36.47861827721907	38.0	37.0	38.0	34.0	38.0
14-15	36.54025652237283	38.0	37.0	38.0	34.0	38.0
16-17	36.333508234951175	38.0	37.0	38.0	34.0	38.0
18-19	36.383865325754265	38.0	37.0	38.0	34.0	38.0
20-21	36.496691444395864	38.0	37.0	38.0	34.0	38.0
22-23	36.352164407520775	38.0	37.0	38.0	34.0	38.0
24-25	36.30208424427926	38.0	37.0	38.0	34.0	38.0
26-27	36.11958898119808	38.0	36.5	38.0	33.5	38.0
28-29	35.997114123305636	38.0	36.5	38.0	32.5	38.0
30-31	35.95990380411018	38.0	37.0	38.0	32.0	38.0
32-33	35.798323859495696	38.0	36.5	38.0	31.5	38.0
34-35	35.54522664334645	38.0	36.0	38.0	31.0	38.0
36-37	35.54102900451829	38.0	36.0	38.0	31.0	38.0
38-39	35.004882670164704	38.0	35.0	38.0	27.0	38.0
40-41	35.64476023903221	38.0	36.0	38.0	31.5	38.0
42-43	35.965544381285525	38.0	36.5	38.0	32.5	38.0
44-45	35.97888062964583	38.0	36.5	38.0	32.0	38.0
46-47	36.013715201865615	38.0	37.0	38.0	32.5	38.0
48-49	35.969465092552106	38.0	36.5	38.0	32.0	38.0
50-51	35.85882524413351	38.0	36.0	38.0	31.5	38.0
52-53	35.88565806733712	38.0	36.5	38.0	31.5	38.0
54-55	35.83840548025069	38.0	37.0	38.0	31.5	38.0
56-57	35.85104212213963	38.0	36.0	38.0	32.0	38.0
58-59	35.71256376621484	38.0	36.0	38.0	31.0	38.0
60-61	35.70720011660108	38.0	36.0	38.0	31.0	38.0
62-63	35.77638828159161	38.0	36.0	38.0	31.0	38.0
64-65	35.81104795219356	38.0	36.0	38.0	31.0	38.0
66-67	35.72193557790409	38.0	36.0	38.0	31.0	38.0
68-69	35.26822620609241	38.0	36.0	38.0	29.5	38.0
70-71	33.79075936452412	37.5	33.0	38.0	22.0	38.0
72-73	31.63575280571345	37.0	26.5	38.0	11.0	38.0
74-75	31.371155808191226	37.0	25.0	38.0	11.0	38.0
76	30.78752368459408	37.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	2.0
23	12.0
24	18.0
25	48.0
26	101.0
27	161.0
28	297.0
29	478.0
30	749.0
31	1142.0
32	1604.0
33	2552.0
34	3840.0
35	5967.0
36	6234.0
37	11097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.06515085264539	16.773065150852645	12.590001457513484	37.57178253898849
2	17.796239615216443	15.618714473108877	43.4951173298353	23.08992858183938
3	17.39979594811252	20.271097507651945	39.75805276198805	22.571053782247485
4	13.511149978137299	27.753971724238447	32.126512170237575	26.608366127386677
5	13.027255502113395	32.76781810231745	40.19239177962396	14.012534615945196
6	26.080746246902788	41.425448185395716	15.499198367584901	16.9946072001166
7	24.465821308847108	26.929019093426614	17.941990963416412	30.66316863430987
8	20.845357819559833	41.60909488412768	16.230870135548752	21.314677160763736
9	19.833843463052034	17.35024048972453	15.172715347616966	47.64320069960647
10-11	22.10319195452558	27.264247194286543	27.264247194286543	23.368313656901325
12-13	22.45882524413351	16.60982364086868	29.056988777146188	31.874362337851625
14-15	21.93412039061361	17.576155079434486	18.396735169800323	42.092989360151584
16-17	30.4955545838799	26.745372394694648	17.115580819122577	25.64349220230287
18-19	30.5028421512899	21.339454889957732	17.386678326774526	30.771024631977845
20-21	22.30724384200554	19.249380556770152	17.45080891998251	40.9925666812418
22-23	23.05932079871739	27.69858621192246	17.35024048972453	31.89185249963562
24-25	30.405188747995922	28.65180002915027	17.65923334790847	23.283777874945343
26-27	29.572948549774086	29.41116455327212	20.24631977845795	20.769567118495846
28-29	21.141233056405774	20.276927561579942	29.32662877131613	29.25521061069815
30-31	21.482291211193704	17.64320069960647	38.969537968226206	21.90497012097362
32-33	27.775834426468442	18.941845212068213	23.589855706165284	29.69246465529806
34-35	22.761988048389448	20.667541174755865	27.360443084098527	29.210027692756157
36-37	20.371665937909924	34.592624981781086	21.54496429091969	23.4907447893893
38-39	20.70543652528786	22.34513919253753	27.781664480396444	29.167759801778164
40-41	20.918233493659816	18.5016761405043	30.29150269639994	30.28858766943594
42-43	29.42573968809212	18.65763008307827	29.5146480104941	22.40198221833552
44-45	21.626585045911675	26.152164407520768	30.190934266141962	22.030316280425595
46-47	29.347033960064127	18.632852353884275	22.571053782247485	29.44905990380411
48-49	28.641597434776273	17.068940387698586	21.824806879463633	32.46465529806151
50-51	21.279696837195743	17.780206966914445	30.419763882815914	30.520332313073894
52-53	19.335373852208132	18.10377495991838	39.059903804110185	23.5009473837633
54-55	19.606471359860077	17.574697565952484	32.775105669727445	30.04372540445999
56-57	27.974056260020408	17.60093280862848	24.70485351989506	29.720157411456054
58-59	21.085847544089784	20.209881941407957	36.20463489287276	22.499635621629498
60-61	27.529514648010494	29.532138172278096	22.725550211339453	20.212796968371958
62-63	19.038041101880193	41.79711412330564	18.424427925958316	20.74041684885585
64-65	18.221833551960355	43.0112228538114	18.14750036437837	20.619443229849878
66-67	17.781664480396444	43.197784579507356	18.538113977554293	20.482436962541904
68-69	17.82830491182043	43.183209444687364	18.170820580090368	20.817665063401837
70-71	18.354467278822327	40.040810377495994	19.19108001749016	22.413642326191518
72-73	19.36306660836613	33.716659379099255	20.94446873633581	25.975805276198805
74-75	19.328086284798136	31.517271534761697	22.624981781081473	26.529660399358697
76	19.18962250400816	31.721323422241653	22.5010931351115	26.587960938638684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.5
16	5.0
17	7.0
18	5.0
19	3.5
20	9.5
21	15.0
22	15.0
23	19.5
24	26.5
25	29.0
26	54.5
27	81.0
28	103.5
29	125.0
30	147.0
31	175.5
32	244.5
33	307.0
34	359.0
35	459.0
36	578.5
37	650.0
38	833.5
39	1954.5
40	3201.0
41	3397.0
42	3284.0
43	2867.0
44	2179.5
45	1714.0
46	1519.0
47	1477.5
48	1321.5
49	1143.5
50	1080.0
51	970.0
52	763.0
53	656.5
54	647.0
55	614.5
56	512.0
57	424.5
58	407.0
59	380.5
60	386.0
61	430.0
62	442.0
63	443.0
64	464.0
65	473.5
66	482.0
67	501.0
68	534.0
69	575.0
70	578.0
71	573.0
72	579.0
73	567.0
74	505.0
75	461.0
76	426.5
77	344.0
78	255.0
79	214.0
80	194.0
81	142.5
82	91.0
83	71.0
84	62.0
85	39.5
86	19.0
87	12.0
88	9.0
89	4.0
90	2.5
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	34305.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.4852062381577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.22069980749632	36.668124180148666
2	17.665043596421697	18.189768255356363
3	5.916657230211754	9.138609532138172
4	2.174159211867286	4.477481416703104
5	1.2682595402559167	3.264830199679347
6	0.6058204053901031	1.8714473108876257
7	0.41897859812025817	1.509983967351698
8	0.16985618842713168	0.6996064713598601
9	0.0905899671611369	0.41976388281591603
>10	0.3906692333824029	2.9820725841714033
>50	0.011323745895142113	0.40227372103191955
>100	0.05095685652813951	4.935140650051013
>500	0.011323745895142113	3.533012680367294
>1k	0.005661872947571056	11.90788514793762
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	4085	11.90788514793762	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	664	1.9355779040956127	RNA PCR Primer, Index 48 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	548	1.5974347762716805	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	349	1.0173444104357965	No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	317	0.9240635475878152	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	254	0.7404168488558519	RNA PCR Primer, Index 48 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	208	0.6063256085118788	No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	125	0.3643783704999271	No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT	123	0.3585483165719283	RNA PCR Primer, Index 48 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	109	0.31773793907593645	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	106	0.3089928581839382	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	102	0.2973327503279405	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	80	0.23320215711995335	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT	58	0.16907156391196618	No Hit
TCTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	45	0.13117621337997376	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT	42	0.1224311324879755	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.002915026963999417	0.0
6	0.0	0.0	0.0	0.002915026963999417	0.0
7	0.0	0.0	0.0	0.002915026963999417	0.0
8	0.0	0.0	0.0	0.002915026963999417	0.0
9	0.0	0.0	0.0	0.00874508089199825	0.0
10	0.0	0.0	0.0	0.00874508089199825	0.0
11	0.0	0.0	0.0	0.014575134819997084	0.0
12	0.0	0.0	0.0	0.014575134819997084	0.0
13	0.0	0.0	0.0	0.014575134819997084	0.0
14	0.0	0.0	0.0	0.014575134819997084	0.0
15	0.0	0.0	0.0	0.014575134819997084	0.0
16	0.0	0.0	0.0	0.014575134819997084	0.0
17	0.0	0.0	0.0	0.0174901617839965	0.0
18	0.0	0.0	0.0	0.0174901617839965	0.0
19	0.0	0.0	0.0	0.032065296603993584	0.0
20	0.0	0.0	0.0	0.037895350531992424	0.0
21	0.0	0.0	0.0	0.04955545838799009	0.0
22	0.0	0.0	0.0	0.04955545838799009	0.0
23	0.0	0.0	0.0	0.052470485351989504	0.0
24	0.0	0.0	0.0	0.05538551231598892	0.0
25	0.0	0.0	0.0	0.058300539279988337	0.0
26	0.0	0.0	0.0	0.06121556624398776	0.0
27	0.0	0.0	0.0	0.08162075499198368	0.0
28	0.0	0.0	0.0	0.13409124034397318	0.0
29	0.0	0.0	0.0	0.1544964290919691	0.0
30	0.0	0.0	0.0	0.19530680658796093	0.0
31	0.0	0.0	0.0	0.23611718408395277	0.0
32	0.0	0.0	0.0	0.2740125346159452	0.0
33	0.0	0.0	0.0	0.3235679930039353	0.0
34	0.0	0.0	0.0	0.39935869406792013	0.0
35	0.0	0.0	0.0	0.5130447456638974	0.0
36	0.0	0.0	0.0	0.6996064713598601	0.0
37	0.0	0.0	0.0	1.014429383471797	0.0
38	0.0	0.0	0.0	1.314677160763737	0.0
39	0.0	0.0	0.0	1.6090948841276782	0.0
40	0.0	0.0	0.0	1.9239177962396152	0.0
41	0.0	0.0	0.0	2.253315843171549	0.0
42	0.0	0.0	0.0	2.6614196181314678	0.0
43	0.0	0.0	0.0	3.046203177379391	0.0
44	0.0	0.0	0.0	3.4222416557353155	0.0
45	0.0	0.0	0.0	3.809940241947238	0.0
46	0.0	0.0	0.0	4.185978720303162	0.0
47	0.0	0.0	0.0	4.588252441335082	0.0
48	0.0	0.0	0.0	5.016761405042996	0.0
49	0.0	0.0	0.0	5.427780206966914	0.0
50	0.0	0.0	0.0	5.830053927998834	0.0
51	0.0	0.0	0.0	6.206092406354759	0.0
52	0.0	0.0	0.0	6.558810668998688	0.0
53	0.0	0.0	0.0	6.969829470922606	0.0
54	0.0	0.0	0.0	7.313802652674537	0.0
55	0.0	0.0	0.0	7.684011077102463	0.0
56	0.0	0.0	0.0	8.086284798134383	0.0
57	0.0	0.0	0.0	8.520623815770296	0.0
58	0.0	0.0	0.0	8.908322401982218	0.0
59	0.0	0.0	0.0	9.29602098819414	0.0
60	0.0	0.0	0.0	9.689549628334062	0.0
61	0.0	0.0	0.0	10.080163241509984	0.0
62	0.0	0.0	0.0	10.377495991837925	0.0
63	0.0	0.0	0.0	10.686488850021863	0.0
64	0.0	0.0	0.0	11.074187436233785	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCCTT	15	0.0022064976	70.0	42
GTAATCG	15	0.0022064976	70.0	36
CTAGTGG	15	0.0022064976	70.0	4
CTATACA	40	7.271774E-7	52.5	1
TTTCCTA	20	0.0068949624	52.5	48
TTACAAA	20	0.0068949624	52.5	29
CTTGAAA	720	0.0	44.236115	57
AATCTCG	670	0.0	43.8806	36
TTGAAAA	720	0.0	43.750004	58
CGTCTTC	730	0.0	43.630135	48
GTCTTCT	730	0.0	43.630135	49
TATGCCG	740	0.0	43.513515	43
ATGCCGT	740	0.0	43.513515	44
CTGCTTG	725	0.0	43.448277	54
TCTTCTG	725	0.0	43.448277	50
CCGTCTT	735	0.0	43.333336	47
GCCGTCT	735	0.0	43.333336	46
TCTCGTA	695	0.0	43.309353	38
TGCCGTC	745	0.0	43.221474	45
CTCGTAT	730	0.0	43.150684	39
>>END_MODULE