FastQCFastQC Report
Wed 25 May 2016
SRR1779765_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779765_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27251
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCC150.00220160470.0000134
TCTGACT150.00220160470.0000111
CATGCAC150.00220160470.0000110
CTTTTCA150.00220160470.0000140
TTCCGAC353.5361154E-970.0000119
TGAGTAG150.00220160470.0000129
GCCATGC150.00220160470.000018
TAGCCAT150.00220160470.000016
TCAATCG150.00220160470.0000147
CTCGGTT150.00220160470.0000144
GTGTACA150.00220160470.0000136
GCGTGTA150.00220160470.0000134
TTCGCCG150.00220160470.0000151
CGTCAAT150.00220160470.0000170
GCACTAC150.00220160470.0000113
TCAACCG150.00220160470.0000132
CGTATCC207.790757E-570.056
CGTGTAG207.790757E-570.035
CGATCTC207.790757E-570.040
CGCTTCC453.619789E-1062.22222516
Produced by FastQC (version 0.11.5)