##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779765_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27251
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.993394737807787	34.0	23.0	34.0	12.0	34.0
2	27.375729331033725	33.0	23.0	34.0	12.0	34.0
3	26.790576492605776	32.0	21.0	34.0	11.0	34.0
4	28.912333492348903	34.0	23.0	34.0	12.0	34.0
5	28.344134160214303	34.0	23.0	34.0	11.0	34.0
6	30.87005981431874	36.0	26.0	38.0	11.0	38.0
7	31.80551172434039	37.0	27.0	38.0	12.0	38.0
8	31.11383068511247	37.0	27.0	38.0	11.0	38.0
9	32.54511761036292	37.0	31.0	38.0	12.0	38.0
10-11	32.127353124655976	37.0	31.0	38.0	11.0	38.0
12-13	31.257109830831897	36.5	28.0	38.0	11.0	38.0
14-15	31.289787530732816	36.5	27.0	38.0	11.0	38.0
16-17	30.978936552787054	36.0	27.0	38.0	11.0	38.0
18-19	31.4132508898756	37.0	27.0	38.0	11.5	38.0
20-21	30.462973835822538	35.5	25.0	38.0	11.0	38.0
22-23	30.49700928406297	35.5	26.0	38.0	11.0	38.0
24-25	31.678910865656306	37.0	29.0	38.0	11.0	38.0
26-27	30.170562548163367	36.0	24.0	38.0	11.0	38.0
28-29	32.243734174892666	37.0	31.0	38.0	11.0	38.0
30-31	32.711955524567905	38.0	33.0	38.0	11.0	38.0
32-33	32.80426406370408	38.0	34.0	38.0	11.0	38.0
34-35	32.859711570217605	38.0	34.0	38.0	11.0	38.0
36-37	30.802319180947485	36.5	25.0	38.0	11.0	38.0
38-39	29.152875123848666	34.0	23.0	37.5	11.0	38.0
40-41	30.329822758797842	35.0	25.0	38.0	11.0	38.0
42-43	31.445084584051962	36.5	28.5	38.0	11.0	38.0
44-45	32.952478808117135	38.0	34.0	38.0	11.0	38.0
46-47	31.532053869582768	37.0	28.5	38.0	11.0	38.0
48-49	30.851069685516126	36.0	27.0	38.0	11.0	38.0
50-51	28.82545594657077	34.0	21.5	38.0	11.0	38.0
52-53	32.02311841767275	37.0	29.5	38.0	11.0	38.0
54-55	31.20795567135151	36.5	27.0	38.0	11.0	38.0
56-57	31.580327327437526	37.0	29.0	38.0	11.0	38.0
58-59	32.68456203442075	37.5	33.0	38.0	11.0	38.0
60-61	33.139976514623314	38.0	34.0	38.0	11.0	38.0
62-63	33.01706359399655	38.0	34.0	38.0	11.0	38.0
64-65	32.75793548860592	38.0	32.5	38.0	11.0	38.0
66-67	30.948845913911416	37.0	25.5	38.0	11.0	38.0
68-69	30.901856812594033	37.0	25.5	38.0	11.0	38.0
70-71	30.285861069318557	36.5	24.0	38.0	11.0	38.0
72-73	29.32505229165902	34.5	22.5	38.0	11.0	38.0
74-75	31.157040108619867	36.5	27.5	38.0	11.0	38.0
76	31.798466111335365	37.0	28.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	10.0
13	68.0
14	174.0
15	315.0
16	465.0
17	710.0
18	802.0
19	763.0
20	764.0
21	552.0
22	415.0
23	356.0
24	336.0
25	320.0
26	353.0
27	401.0
28	457.0
29	615.0
30	706.0
31	970.0
32	1499.0
33	2011.0
34	2964.0
35	4085.0
36	4721.0
37	2417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.668562621555175	22.923929397086347	12.322483578584272	33.08502440277421
2	17.412205056695168	17.151664159113427	42.09019852482478	23.345932259366627
3	19.276356830941985	17.793842427800815	37.49954130123665	25.430259440020546
4	13.056401599941287	20.461634435433563	33.78224652306338	32.69971744156177
5	13.548126674250485	28.971413893068142	40.479248467946135	17.00121096473524
6	25.323841326923784	34.46845987303218	16.542512201387105	23.66518659865693
7	24.028476019228652	24.964221496458848	22.718432351106383	28.288870133206125
8	20.608418039704965	32.96759751935709	18.24520201093538	28.17878243000257
9	21.65425122013871	14.803126490770982	20.212102308172177	43.33051998091813
10-11	23.213827015522366	23.8688488495835	27.4833951047668	25.433929030127334
12-13	22.347803750321088	19.38460973909214	30.365858133646473	27.901728376940294
14-15	23.037686690396683	22.86338116032439	23.553264100399986	30.545668048878937
16-17	24.784411581226376	22.55146600124766	25.624747715680158	27.0393747018458
18-19	21.026751311878463	22.52761366555356	28.444827712744488	28.000807309823493
20-21	24.364243513999487	22.202854941103077	26.296282705221824	27.13661883967561
22-23	25.101831125463285	21.49278925544017	26.67975487138087	26.72562474771568
24-25	21.540493926828375	24.93853436571135	24.043154379655792	29.477817327804484
26-27	23.39730652086162	23.756926351326555	26.7696598289971	26.076107298814723
28-29	22.257898792704857	24.492679167736963	25.77520091005835	27.47422112949984
30-31	22.04506256651132	24.450478881508936	26.388022457891452	27.116436094088293
32-33	22.002862280283292	24.474331217203037	26.287108729954866	27.235697772558805
34-35	22.265237972918424	24.536714249018384	26.074272503761332	27.12377527430186
36-37	24.51102711827089	21.66892958056585	29.899820190084768	23.920223111078492
38-39	24.07618069061686	20.72034053796191	33.02631096106565	22.177167810355584
40-41	22.978973248688124	23.678030164030677	25.013760962900445	28.329235624380754
42-43	20.27081574988074	24.516531503431064	26.88525191736083	28.327400829327367
44-45	19.883673993614913	23.547759715239806	28.79527356794246	27.773292723202818
46-47	23.808300612821547	22.393673626655904	27.91824153242083	25.87978422810172
48-49	23.604638361894974	22.729441121426735	28.743899306447467	24.922021210230817
50-51	26.80819052511834	20.593739679277824	29.655792447983558	22.942277347620273
52-53	20.094675424755053	24.17159003339327	28.088877472386336	27.64485706946534
54-55	22.74044989174709	22.96246009320759	27.40449891747092	26.892591097574403
56-57	22.933103372353308	22.97163406847455	26.778833804264064	27.316428754908078
58-59	20.889141682874023	24.19177277898059	27.439360023485378	27.479725514660014
60-61	20.219441488385748	23.522072584492314	27.156801585262926	29.101684341859013
62-63	19.77909067557154	24.77156801585263	27.00818318593813	28.4411581226377
64-65	20.766591438689197	25.305957688849745	26.51327498578008	27.41417588668098
66-67	19.329565887490368	26.250412828887015	23.153278778760413	31.266742504862204
68-69	19.158149690820014	26.872052698214645	23.400429365676434	30.569368245288903
70-71	19.80661260137243	26.237569263513265	23.560603280613556	30.39521485450075
72-73	19.463505926388024	26.24857803383362	21.903783347400097	32.38413269237826
74-75	20.747862463762797	26.110968404829183	24.615610436314263	28.525558695093757
76	22.63770136875711	24.644967157168544	25.140361821584527	27.57696965248982
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.0
16	2.0
17	3.0
18	3.5
19	3.0
20	6.0
21	10.0
22	14.5
23	26.0
24	33.5
25	34.0
26	51.0
27	84.5
28	122.5
29	144.0
30	181.0
31	254.5
32	331.5
33	372.0
34	440.5
35	541.0
36	654.5
37	736.0
38	815.0
39	972.5
40	1144.0
41	1307.0
42	1377.0
43	1465.0
44	1588.5
45	1592.5
46	1561.0
47	1560.0
48	1530.5
49	1392.0
50	1282.0
51	1204.5
52	1079.0
53	957.5
54	884.0
55	813.5
56	700.0
57	626.0
58	595.0
59	552.5
60	484.0
61	446.0
62	434.0
63	410.5
64	413.0
65	433.0
66	405.5
67	384.0
68	378.0
69	378.5
70	368.0
71	351.0
72	323.5
73	277.5
74	236.5
75	214.0
76	195.0
77	154.5
78	106.5
79	80.0
80	66.0
81	46.5
82	31.5
83	22.0
84	17.5
85	11.0
86	7.0
87	5.0
88	5.0
89	2.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.001834795053392536
66-67	0.0
68-69	0.005504385160177609
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	27251.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.12483945543283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.41062079281974	96.56526366004917
2	1.3425579655946147	2.634765696671682
3	0.1907255048616305	0.561447286338116
4	0.037397157816005985	0.14678360427140288
5	0.018698578908002993	0.0917397526696268
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.003669590106785072	0.0
18	0.0	0.0	0.0	0.003669590106785072	0.0
19	0.0	0.0	0.0	0.007339180213570144	0.0
20	0.0	0.0	0.0	0.007339180213570144	0.0
21	0.0	0.0	0.0	0.014678360427140288	0.0
22	0.0	0.0	0.0	0.05871344170856115	0.0
23	0.0	0.0	0.0	0.05871344170856115	0.0
24	0.0	0.0	0.0	0.08807016256284173	0.0
25	0.0	0.0	0.0	0.08807016256284173	0.0
26	0.0	0.0	0.0	0.08807016256284173	0.0
27	0.0	0.0	0.0	0.10274852298998202	0.0
28	0.0	0.0	0.0	0.11375729331033724	0.0
29	0.0	0.0	0.0	0.12109647352390739	0.0
30	0.0	0.0	0.0	0.12109647352390739	0.0
31	0.0	0.0	0.0	0.12109647352390739	0.0
32	0.0	0.0	0.0	0.15045319437818797	0.0
33	0.0	0.0	0.0	0.16513155480532823	0.0
34	0.0	0.0	0.0	0.1834795053392536	0.0
35	0.0	0.0	0.0	0.24586253715459983	0.0
36	0.0	0.0	0.0	0.2788888481156655	0.0
37	0.0	0.0	0.0	0.407324501853143	0.0
38	0.0	0.0	0.0	0.49539466441598473	0.0
39	0.0	0.0	0.0	0.6385086785806026	0.0
40	0.0	0.0	0.0	0.7742835125316502	0.0
41	0.0	0.0	0.0	0.8807016256284173	0.0
42	0.0	0.0	0.0	1.1008770320355217	0.0
43	0.0	0.0	0.0	1.27334776705442	0.0
44	0.0	0.0	0.0	1.4421489119665334	0.0
45	0.0	0.0	0.0	1.6072804667718616	0.0
46	0.0	0.0	0.0	1.8054383325382555	0.0
47	0.0	0.0	0.0	2.091666360867491	0.0
48	0.0	0.0	0.0	2.2164324244981835	0.0
49	0.0	0.0	0.0	2.3595464386628016	0.0
50	0.0	0.0	0.0	2.653113647205607	0.0
51	0.0	0.0	0.0	2.8659498733991415	0.0
52	0.0	0.0	0.0	3.108142820446956	0.0
53	0.0	0.0	0.0	3.342996587281201	0.0
54	0.0	0.0	0.0	3.658581336464717	0.0
55	0.0	0.0	0.0	3.860408792337896	0.0
56	0.0	0.0	0.0	4.08792337895857	0.0
57	0.0	0.0	0.0	4.31176837547246	0.0
58	0.0	0.0	0.0	4.454882389637078	0.0
59	0.0	0.0	0.0	4.671388205937397	0.0
60	0.0	0.0	0.0	4.862206891490221	0.0
61	0.0	0.0	0.0	5.034677626509119	0.0
62	0.0	0.0	0.0	5.166782870353382	0.0
63	0.0	0.0	0.0	5.295218524090859	0.0
64	0.0	0.0	0.0	5.416314997614767	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCC	15	0.002201604	70.00001	34
TCTGACT	15	0.002201604	70.00001	11
CATGCAC	15	0.002201604	70.00001	10
CTTTTCA	15	0.002201604	70.00001	40
TTCCGAC	35	3.5361154E-9	70.00001	19
TGAGTAG	15	0.002201604	70.00001	29
GCCATGC	15	0.002201604	70.00001	8
TAGCCAT	15	0.002201604	70.00001	6
TCAATCG	15	0.002201604	70.00001	47
CTCGGTT	15	0.002201604	70.00001	44
GTGTACA	15	0.002201604	70.00001	36
GCGTGTA	15	0.002201604	70.00001	34
TTCGCCG	15	0.002201604	70.00001	51
CGTCAAT	15	0.002201604	70.00001	70
GCACTAC	15	0.002201604	70.00001	13
TCAACCG	15	0.002201604	70.00001	32
CGTATCC	20	7.790757E-5	70.0	56
CGTGTAG	20	7.790757E-5	70.0	35
CGATCTC	20	7.790757E-5	70.0	40
CGCTTCC	45	3.619789E-10	62.222225	16
>>END_MODULE