FastQCFastQC Report
Wed 25 May 2016
SRR1779765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27251
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT16986.230964001321052No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT5662.076988000440351TruSeq Adapter, Index 2 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2991.0972074419287365No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG2650.972441378298044No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2220.8146490037062861No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2040.7485963817841547No Hit
TATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCT1030.3779677809988624No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC970.355950240358152No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG910.33393269971744155No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG830.304575978863161No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC820.30090638875637593No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT530.19448827565960883No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT500.1834795053392536No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC460.16880114491211332No Hit
TCTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT430.1577923745917581No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA352.8687828E-760.0000042
TATGAGG200.006879788452.551
TCTTTAT407.2158946E-752.51
TATAGCA200.006879788452.52
CGCCGCG200.006879788452.554
AAACCAA305.7783874E-446.66666870
CTATACA503.3570614E-642.01
TTTATAC503.3570614E-642.03
CTTTACA350.00123432240.0000041
TTCTGCT4900.035.71428352
GTCTTCT4900.035.71428349
CTGCTTG4900.035.71428354
TCTTCTG4900.035.71428350
CGTCTTC5000.035.748
ATCTCGT4750.035.36842337
TCTCGTA4750.035.36842338
TCTGCTT4950.035.35353553
CCGTCTT5050.035.34653547
GCCGTCT5050.035.34653546
CTCGTAT5050.035.34653539