##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779765_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27251 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.265935195038715 34.0 34.0 34.0 32.0 34.0 2 33.29433782246523 34.0 34.0 34.0 33.0 34.0 3 33.36006018127775 34.0 34.0 34.0 33.0 34.0 4 33.41822318447029 34.0 34.0 34.0 34.0 34.0 5 33.36607830905288 34.0 34.0 34.0 34.0 34.0 6 36.65865472826685 38.0 37.0 38.0 34.0 38.0 7 36.76672415691167 38.0 37.0 38.0 34.0 38.0 8 36.35716120509339 38.0 37.0 38.0 34.0 38.0 9 36.47466148031265 38.0 37.0 38.0 34.0 38.0 10-11 36.32393306667645 38.0 37.0 38.0 34.0 38.0 12-13 36.3283916186562 38.0 37.0 38.0 34.0 38.0 14-15 36.3935084951011 38.0 37.0 38.0 34.0 38.0 16-17 36.22511100510073 38.0 36.5 38.0 33.5 38.0 18-19 36.27492569080034 38.0 37.0 38.0 34.0 38.0 20-21 36.34817070933177 38.0 37.0 38.0 34.0 38.0 22-23 36.1994055264027 38.0 37.0 38.0 34.0 38.0 24-25 36.13874720193754 38.0 36.0 38.0 33.0 38.0 26-27 35.95611170232285 38.0 36.0 38.0 31.5 38.0 28-29 35.74980734651939 38.0 36.0 38.0 31.5 38.0 30-31 35.47878976918278 38.0 36.0 38.0 31.0 38.0 32-33 35.49264247183589 38.0 36.0 38.0 31.0 38.0 34-35 35.44884591391141 38.0 36.0 38.0 31.0 38.0 36-37 35.397930351179774 38.0 36.0 38.0 31.0 38.0 38-39 35.16004917250743 38.0 36.0 38.0 28.5 38.0 40-41 35.63716927819163 38.0 36.0 38.0 31.5 38.0 42-43 35.824942203955814 38.0 36.0 38.0 31.5 38.0 44-45 35.79918168140619 38.0 36.0 38.0 31.0 38.0 46-47 35.80395214854501 38.0 36.0 38.0 31.0 38.0 48-49 35.787035338152734 38.0 36.0 38.0 31.0 38.0 50-51 35.670250633004294 38.0 36.0 38.0 31.0 38.0 52-53 35.67793842427801 38.0 36.0 38.0 31.0 38.0 54-55 35.69571758834538 38.0 36.0 38.0 31.0 38.0 56-57 35.629518182818984 38.0 36.0 38.0 31.0 38.0 58-59 35.54790649884408 38.0 36.0 38.0 31.0 38.0 60-61 35.54843858940956 38.0 36.0 38.0 31.0 38.0 62-63 35.61124362408719 38.0 36.0 38.0 31.0 38.0 64-65 35.65652636600492 38.0 36.0 38.0 31.0 38.0 66-67 35.63482074052328 38.0 36.0 38.0 31.0 38.0 68-69 35.12364683864813 38.0 36.0 38.0 28.0 38.0 70-71 34.099812850904556 37.5 34.5 38.0 23.5 38.0 72-73 32.79982385967487 37.0 33.0 38.0 15.0 38.0 74-75 32.60788594913948 38.0 33.0 38.0 11.0 38.0 76 31.853620050640345 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 3.0 22 9.0 23 14.0 24 31.0 25 53.0 26 85.0 27 171.0 28 248.0 29 416.0 30 619.0 31 879.0 32 1353.0 33 1994.0 34 3047.0 35 4315.0 36 5284.0 37 8729.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.30145682727239 20.12770173571612 13.192176433892334 31.37866500311915 2 18.292906682323583 18.380976844886426 41.89937983927196 21.426736633518036 3 17.911269311217936 24.1642508531797 36.29958533631793 21.62489449928443 4 13.584822575318336 24.70001100877032 35.68309419837804 26.032072217533305 5 12.663755458515283 37.02983376756816 37.473854170489155 12.832556603427397 6 26.42838794906609 40.10861986716084 16.810392279182416 16.652599904590655 7 24.3367215881986 30.60805108069429 20.564382958423543 24.490844372683572 8 20.982716230597042 40.11228945726762 17.84888627940259 21.05610803273274 9 21.859748266118675 17.478257678617297 18.16080143847932 42.50119261678471 10-11 22.911085831712597 26.7953469597446 28.028329235624383 22.265237972918424 12-13 23.861509669369934 19.04150306410774 28.16593886462882 28.931048401893513 14-15 23.278044842391104 20.391912223404645 20.861619757073136 35.468423177131115 16-17 28.12924296356097 27.01919195625849 19.876334813401343 24.9752302667792 18-19 26.976991670030458 24.12205056695167 20.74419287365601 28.156764889361856 20-21 22.60100546768926 22.823015669149758 20.379068658030896 34.19691020513009 22-23 24.010128068694726 27.832006164911377 20.507504311768376 27.650361454625518 24-25 27.872371656086013 29.035631719936884 20.490991156287844 22.60100546768926 26-27 28.002642104876884 29.75670617592015 20.91482881362152 21.325822905581447 28-29 28.67784668452534 24.032145609335437 21.85607867601189 25.433929030127334 30-31 28.428314557263956 27.34762027081575 21.31481413526109 22.909251036659207 32-33 23.88352721001064 28.052181571318485 21.83406113537118 26.230230083299695 34-35 26.931121793695645 24.356904333785916 23.26703607207075 25.444937800447693 36-37 23.228505375949506 26.57150196323071 28.56225459616161 21.637738064658176 38-39 21.42306704341125 23.136765623279878 29.12003229239294 26.320135040915932 40-41 22.63770136875711 20.82859344611207 29.573226670580897 26.960478514549923 42-43 27.112766503981504 21.48911966533338 29.745697405599795 21.65241642508532 44-45 22.426699937616966 26.354996146930386 29.231954790649883 21.98634912480276 46-47 27.626509118931413 21.52581556640123 24.42295695570805 26.424718358959304 48-49 27.120105684195075 20.24329382407985 23.98260614289384 28.653994348831237 50-51 22.107445598326667 20.41759935415214 29.998899122967966 27.47605592455323 52-53 21.42306704341125 20.48915636123445 35.251917360830795 22.835859234523504 54-55 21.270779053979673 19.979083336391326 31.815346225826573 26.93479138380243 56-57 26.12564676525632 20.399251403618216 27.02836593152545 26.446735899600014 58-59 21.94414883857473 23.342262669259846 32.523577116436094 22.19001137572933 60-61 25.66144361674801 30.235587684855602 24.07618069061686 20.02678800777953 62-63 20.074492679167736 37.954570474478 21.16069135077612 20.810245495578144 64-65 19.735055594290117 39.13984807896958 20.933176764155444 20.191919562584857 66-67 19.784595060731718 38.61326189864592 20.808410700524753 20.793732340097613 68-69 19.472679901654985 38.89398554181498 20.782723569777257 20.85061098675278 70-71 20.05064034347363 36.7747972551466 21.28545741440681 21.889104986972953 72-73 20.37356427287072 32.63549961469304 22.279916333345565 24.711019779090677 74-75 20.588235294117645 31.186011522512935 23.252357711643608 24.97339547172581 76 21.17720450625665 31.044732303401712 22.641370958863895 25.136692231477742 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.5 16 3.5 17 5.0 18 4.5 19 7.0 20 11.5 21 13.0 22 21.0 23 36.5 24 57.5 25 71.0 26 84.0 27 101.0 28 134.5 29 164.0 30 201.5 31 250.0 32 341.0 33 421.0 34 461.5 35 536.0 36 644.0 37 718.0 38 749.5 39 914.0 40 1163.0 41 1830.0 42 2381.0 43 2492.0 44 2532.5 45 2184.0 46 1906.0 47 1729.0 48 1371.5 49 1121.5 50 1052.0 51 925.0 52 670.0 53 533.5 54 525.0 55 492.5 56 433.0 57 377.0 58 348.0 59 317.5 60 277.0 61 277.0 62 287.0 63 281.5 64 284.0 65 290.0 66 305.0 67 322.0 68 337.5 69 359.0 70 347.0 71 329.0 72 319.5 73 300.5 74 268.5 75 246.0 76 241.5 77 201.0 78 145.5 79 126.0 80 100.5 81 69.0 82 59.0 83 55.0 84 34.5 85 9.5 86 5.0 87 5.0 88 3.0 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 27251.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.73178965909508 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0140581068416 44.057098822061576 2 14.901593252108716 17.503944809364793 3 5.429553264604811 9.566621408388682 4 2.105592002499219 4.946607463946277 5 1.1184004998437989 3.2842831455726396 6 0.49984379881287094 1.7614032512568347 7 0.331146516713527 1.3614179296172617 8 0.17494532958450484 0.8219881839198562 9 0.09372071227741331 0.4953946644159848 >10 0.2561699468915964 2.392572749623867 >50 0.031240237425804434 1.4898535833547393 >100 0.031240237425804434 4.010861986716084 >500 0.006248047485160888 2.076988000440351 >1k 0.006248047485160888 6.230964001321052 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 1698 6.230964001321052 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 566 2.076988000440351 TruSeq Adapter, Index 2 (95% over 21bp) CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 299 1.0972074419287365 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 265 0.972441378298044 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 222 0.8146490037062861 No Hit CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 204 0.7485963817841547 No Hit TATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCT 103 0.3779677809988624 No Hit TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 97 0.355950240358152 No Hit CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 91 0.33393269971744155 No Hit CTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 83 0.304575978863161 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 82 0.30090638875637593 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT 53 0.19448827565960883 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT 50 0.1834795053392536 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 46 0.16880114491211332 No Hit TCTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 43 0.1577923745917581 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.003669590106785072 0.0 18 0.0 0.0 0.0 0.003669590106785072 0.0 19 0.0 0.0 0.0 0.011008770320355216 0.0 20 0.0 0.0 0.0 0.011008770320355216 0.0 21 0.0 0.0 0.0 0.014678360427140288 0.0 22 0.0 0.0 0.0 0.01834795053392536 0.0 23 0.0 0.0 0.0 0.01834795053392536 0.0 24 0.0 0.0 0.0 0.01834795053392536 0.0 25 0.0 0.0 0.0 0.02201754064071043 0.0 26 0.0 0.0 0.0 0.029356720854280576 0.0 27 0.0 0.0 0.0 0.05137426149499101 0.0 28 0.0 0.0 0.0 0.05871344170856115 0.0 29 0.0 0.0 0.0 0.0660526219221313 0.0 30 0.0 0.0 0.0 0.07339180213570144 0.0 31 0.0 0.0 0.0 0.08807016256284173 0.0 32 0.0 0.0 0.0 0.11008770320355216 0.0 33 0.0 0.0 0.0 0.13944442405783275 0.0 34 0.0 0.0 0.0 0.17980991523246853 0.0 35 0.0 0.0 0.0 0.2605408975817401 0.0 36 0.0 0.0 0.0 0.355950240358152 0.0 37 0.0 0.0 0.0 0.5908040071923966 0.0 38 0.0 0.0 0.0 0.7229092510366592 0.0 39 0.0 0.0 0.0 0.9320758871234083 0.0 40 0.0 0.0 0.0 1.1375729331033724 0.0 41 0.0 0.0 0.0 1.2880261274815603 0.0 42 0.0 0.0 0.0 1.5485670250633004 0.0 43 0.0 0.0 0.0 1.8091079226450406 0.0 44 0.0 0.0 0.0 2.029283329052145 0.0 45 0.0 0.0 0.0 2.3118417672745952 0.0 46 0.0 0.0 0.0 2.6274265164581116 0.0 47 0.0 0.0 0.0 2.9833767568162637 0.0 48 0.0 0.0 0.0 3.2329088840776485 0.0 49 0.0 0.0 0.0 3.54482404315438 0.0 50 0.0 0.0 0.0 3.860408792337896 0.0 51 0.0 0.0 0.0 4.106271329492496 0.0 52 0.0 0.0 0.0 4.429195258889582 0.0 53 0.0 0.0 0.0 4.6750577960441815 0.0 54 0.0 0.0 0.0 4.997981725441268 0.0 55 0.0 0.0 0.0 5.3098968845179995 0.0 56 0.0 0.0 0.0 5.618142453487946 0.0 57 0.0 0.0 0.0 5.959414333418957 0.0 58 0.0 0.0 0.0 6.216285640893912 0.0 59 0.0 0.0 0.0 6.509852849436718 0.0 60 0.0 0.0 0.0 6.86580308979487 0.0 61 0.0 0.0 0.0 7.181387838978386 0.0 62 0.0 0.0 0.0 7.500642178268687 0.0 63 0.0 0.0 0.0 7.779531026384353 0.0 64 0.0 0.0 0.0 8.065759054713588 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTATA 35 2.8687828E-7 60.000004 2 TATGAGG 20 0.0068797884 52.5 51 TCTTTAT 40 7.2158946E-7 52.5 1 TATAGCA 20 0.0068797884 52.5 2 CGCCGCG 20 0.0068797884 52.5 54 AAACCAA 30 5.7783874E-4 46.666668 70 CTATACA 50 3.3570614E-6 42.0 1 TTTATAC 50 3.3570614E-6 42.0 3 CTTTACA 35 0.001234322 40.000004 1 TTCTGCT 490 0.0 35.714283 52 GTCTTCT 490 0.0 35.714283 49 CTGCTTG 490 0.0 35.714283 54 TCTTCTG 490 0.0 35.714283 50 CGTCTTC 500 0.0 35.7 48 ATCTCGT 475 0.0 35.368423 37 TCTCGTA 475 0.0 35.368423 38 TCTGCTT 495 0.0 35.353535 53 CCGTCTT 505 0.0 35.346535 47 GCCGTCT 505 0.0 35.346535 46 CTCGTAT 505 0.0 35.346535 39 >>END_MODULE