FastQCFastQC Report
Wed 25 May 2016
SRR1779764_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779764_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15584
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCCG150.00218374770.048
GTATCAT150.00218374770.057
TGCCTAC207.685639E-570.019
AGTGTAG207.685639E-570.035
GATCTCG252.7113474E-670.041
CGTGTAT150.00218374770.035
CTGCCTA150.00218374770.018
ATCCGAC150.00218374770.010
AGATCTC150.00218374770.040
TCTCGGT453.45608E-1062.22221843
ATCTCGG352.788256E-760.00000442
TCGGTGG352.788256E-760.00000445
CGCTTCC352.788256E-760.00000416
CGGTGGT352.788256E-760.00000446
CCGTATC307.999513E-658.33333255
TGTAGAT307.999513E-658.33333237
CGTATCA252.3181946E-455.99999656
TTACGCT252.3181946E-455.99999613
CTCGGTG452.4791007E-854.44444344
CTCTGCC200.006824409652.516
Produced by FastQC (version 0.11.5)