##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779764_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15584
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.31038244353183	32.0	23.0	34.0	12.0	34.0
2	26.316927618069816	31.0	21.0	34.0	12.0	34.0
3	25.61729979466119	31.0	21.0	34.0	11.0	34.0
4	27.365118069815196	32.0	23.0	34.0	12.0	34.0
5	26.88501026694045	32.0	21.0	34.0	11.0	34.0
6	28.92113706365503	34.0	21.0	38.0	11.0	38.0
7	29.900154004106778	34.0	24.0	38.0	12.0	38.0
8	29.201296201232033	34.0	21.0	38.0	11.0	38.0
9	30.805056468172484	36.0	26.0	38.0	12.0	38.0
10-11	30.203349589322382	36.0	24.0	38.0	11.0	38.0
12-13	29.65397202258727	35.5	23.5	38.0	11.0	38.0
14-15	29.30008341889117	34.5	22.0	38.0	11.0	38.0
16-17	29.19728567761807	35.0	23.5	38.0	11.0	38.0
18-19	29.64659265913758	34.5	23.0	38.0	11.0	38.0
20-21	28.662955595482543	33.0	22.0	37.5	11.0	38.0
22-23	28.653009496919918	33.5	22.5	37.5	11.0	38.0
24-25	29.582937628336758	35.0	22.5	38.0	11.0	38.0
26-27	28.287698921971252	33.0	21.5	38.0	11.0	38.0
28-29	30.00506930184805	36.5	23.0	38.0	11.0	38.0
30-31	30.464931981519506	37.0	24.0	38.0	11.0	38.0
32-33	30.693435574948666	37.0	25.5	38.0	11.0	38.0
34-35	30.738513860369608	37.0	26.0	38.0	11.0	38.0
36-37	28.873716632443532	34.0	22.0	38.0	11.0	38.0
38-39	27.759914014373717	32.0	21.0	37.0	11.0	38.0
40-41	28.395309291581107	34.0	21.0	37.0	11.0	38.0
42-43	29.335632700205338	35.0	23.0	38.0	11.0	38.0
44-45	30.73078157084189	37.0	26.0	38.0	11.0	38.0
46-47	29.503850102669404	35.5	23.5	38.0	11.0	38.0
48-49	28.79260780287474	35.0	22.0	38.0	11.0	38.0
50-51	27.034554671457904	31.0	16.0	37.0	11.0	38.0
52-53	29.776694045174537	35.5	23.5	38.0	11.0	38.0
54-55	29.000609599589325	35.0	22.0	38.0	11.0	38.0
56-57	29.46534907597536	35.5	23.0	38.0	11.0	38.0
58-59	30.324082392197127	37.0	23.5	38.0	11.0	38.0
60-61	30.86579183778234	37.0	25.0	38.0	11.0	38.0
62-63	30.712846509240244	37.0	24.5	38.0	11.0	38.0
64-65	30.476514373716633	37.0	24.0	38.0	11.0	38.0
66-67	29.004074691991786	34.5	21.5	38.0	11.0	38.0
68-69	28.80200847022587	34.0	21.0	38.0	11.0	38.0
70-71	28.356038244353183	34.0	21.0	38.0	11.0	38.0
72-73	27.406121663244356	32.0	20.0	37.0	11.0	38.0
74-75	28.78365631416838	35.0	22.0	37.5	11.0	38.0
76	29.254299281314168	36.0	22.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	15.0
13	43.0
14	151.0
15	266.0
16	379.0
17	575.0
18	735.0
19	754.0
20	726.0
21	509.0
22	367.0
23	318.0
24	256.0
25	217.0
26	209.0
27	244.0
28	312.0
29	337.0
30	421.0
31	575.0
32	817.0
33	1075.0
34	1471.0
35	1927.0
36	1934.0
37	950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.63603696098563	18.069815195071868	12.403747433264888	36.89040041067762
2	17.46021560574949	14.803644763860369	45.110369609856264	22.62577002053388
3	17.466632443531825	17.20354209445585	40.734086242299796	24.595739219712527
4	14.046457905544148	20.546714579055443	29.119609856262834	36.287217659137575
5	14.104209445585218	24.69199178644764	41.061344969199176	20.142453798767967
6	24.25564681724846	34.715092402464066	15.7661704312115	25.263090349075974
7	23.607546201232033	23.177618069815196	20.16170431211499	33.053131416837786
8	20.60446611909651	32.7258726899384	17.967145790554415	28.70251540041068
9	20.829055441478438	14.983316221765913	19.443018480492814	44.744609856262834
10-11	22.46214065708419	22.468557494866527	28.28221252566735	26.78708932238193
12-13	21.631160164271044	17.161832648870636	31.285292607802873	29.921714579055443
14-15	23.96368069815195	21.335985626283367	23.382956878850102	31.31737679671458
16-17	27.02130390143737	19.78952772073922	23.95084702258727	29.238321355236142
18-19	21.522073921971252	19.529645790554415	28.55813655030801	30.390143737166326
20-21	26.617043121149898	19.542479466119097	25.118711498973305	28.7217659137577
22-23	27.91003593429158	18.413116016427107	24.26527207392197	29.411575975359344
24-25	21.6472022587269	23.816093429158112	21.974460985626283	32.562243326488705
26-27	25.978567761806982	22.25680184804928	24.752951745379878	27.011678644763858
28-29	23.73588295687885	22.738064681724847	24.271688911704313	29.25436344969199
30-31	22.757315195071868	22.661062628336754	25.413886036960985	29.16773613963039
32-33	22.484599589322382	22.487808008213552	25.66093429158111	29.36665811088296
34-35	23.28349589322382	22.26642710472279	25.240631416837783	29.209445585215605
36-37	26.969969199178646	18.547869609856264	29.700333675564682	24.781827515400412
38-39	25.94969199178645	17.41208932238193	33.95469712525667	22.68352156057495
40-41	24.043891170431213	23.36370636550308	24.043891170431213	28.548511293634498
42-43	19.975616016427107	23.64283880903491	26.479081108829565	29.90246406570842
44-45	20.437628336755647	20.47292094455852	29.16452772073922	29.924922997946613
46-47	26.41491273100616	19.04838295687885	27.01488706365503	27.521817248459957
48-49	25.8791067761807	19.475102669404517	28.73459958932238	25.911190965092402
50-51	31.824306981519506	17.652720739219713	27.02451232032854	23.498459958932237
52-53	19.96278234086242	21.246149897330596	28.664014373716633	30.127053388090346
54-55	25.503721765913756	20.30929158110883	25.81172997946612	28.375256673511295
56-57	25.019250513347025	20.367043121149898	25.176463039014372	29.437243326488705
58-59	20.9958932238193	22.718814168377826	26.902592402464066	29.382700205338807
60-61	20.52104722792608	20.665426078028748	27.399897330595486	31.41362936344969
62-63	20.190580082135522	22.866401437371664	26.91221765913758	30.030800821355236
64-65	20.16491273100616	24.066350102669404	26.225616016427107	29.543121149897328
66-67	19.305056468172484	27.23305954825462	20.97664271047228	32.48524127310062
68-69	19.335066268733353	28.201919065498537	21.218831231346876	31.24418343442123
70-71	19.6291067761807	28.805184804928132	20.501796714579054	31.063911704312115
72-73	19.366016427104725	29.190195071868587	19.654774127310063	31.789014373716633
74-75	21.021560574948666	25.529389117043124	23.135908624229977	30.313141683778234
76	23.27387063655031	22.08033880903491	24.58290554414784	30.062885010266942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	0.5
17	0.0
18	0.0
19	1.0
20	4.0
21	6.0
22	7.5
23	9.5
24	20.0
25	30.0
26	32.0
27	45.5
28	68.5
29	80.0
30	94.5
31	129.0
32	159.5
33	170.0
34	216.0
35	272.0
36	319.5
37	357.0
38	390.5
39	469.0
40	558.5
41	648.5
42	694.0
43	718.5
44	749.0
45	742.0
46	729.0
47	732.5
48	718.0
49	659.5
50	619.0
51	613.5
52	581.5
53	537.0
54	519.0
55	481.0
56	422.5
57	387.5
58	373.0
59	339.5
60	331.5
61	353.0
62	349.0
63	357.0
64	360.0
65	353.0
66	352.0
67	353.0
68	333.5
69	328.0
70	325.5
71	309.0
72	295.0
73	258.0
74	211.0
75	187.0
76	174.0
77	140.0
78	99.0
79	79.0
80	71.0
81	48.0
82	26.5
83	20.0
84	16.0
85	9.0
86	5.0
87	4.0
88	4.5
89	2.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.02245893223819302
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	15584.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.14014373716633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.2168284789644	98.36370636550308
2	0.7119741100323626	1.4117043121149897
3	0.05825242718446602	0.1732546201232033
4	0.012944983818770225	0.051334702258726904
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006416837782340862	0.0
2	0.0	0.0	0.0	0.006416837782340862	0.0
3	0.0	0.0	0.0	0.006416837782340862	0.0
4	0.0	0.0	0.0	0.006416837782340862	0.0
5	0.0	0.0	0.0	0.006416837782340862	0.0
6	0.0	0.0	0.0	0.006416837782340862	0.0
7	0.0	0.0	0.0	0.006416837782340862	0.0
8	0.0	0.0	0.0	0.006416837782340862	0.0
9	0.0	0.0	0.0	0.006416837782340862	0.0
10	0.0	0.0	0.0	0.006416837782340862	0.0
11	0.0	0.0	0.0	0.006416837782340862	0.0
12	0.0	0.0	0.0	0.006416837782340862	0.0
13	0.0	0.0	0.0	0.006416837782340862	0.0
14	0.0	0.0	0.0	0.012833675564681724	0.0
15	0.0	0.0	0.0	0.012833675564681724	0.0
16	0.0	0.0	0.0	0.012833675564681724	0.0
17	0.0	0.0	0.0	0.012833675564681724	0.0
18	0.0	0.0	0.0	0.012833675564681724	0.0
19	0.0	0.0	0.0	0.019250513347022588	0.0
20	0.0	0.0	0.0	0.03208418891170431	0.0
21	0.0	0.0	0.0	0.038501026694045176	0.0
22	0.0	0.0	0.0	0.1540041067761807	0.0
23	0.0	0.0	0.0	0.1540041067761807	0.0
24	0.0	0.0	0.0	0.16042094455852157	0.0
25	0.0	0.0	0.0	0.1668377823408624	0.0
26	0.0	0.0	0.0	0.1668377823408624	0.0
27	0.0	0.0	0.0	0.1668377823408624	0.0
28	0.0	0.0	0.0	0.1668377823408624	0.006416837782340862
29	0.0	0.0	0.0	0.1668377823408624	0.006416837782340862
30	0.0	0.0	0.0	0.17967145790554415	0.006416837782340862
31	0.0	0.0	0.0	0.186088295687885	0.006416837782340862
32	0.0	0.0	0.0	0.186088295687885	0.006416837782340862
33	0.0	0.0	0.0	0.19892197125256675	0.006416837782340862
34	0.0	0.0	0.0	0.26309034907597534	0.006416837782340862
35	0.0	0.0	0.0	0.3080082135523614	0.006416837782340862
36	0.0	0.0	0.0	0.32725872689938396	0.006416837782340862
37	0.0	0.0	0.0	0.4940965092402464	0.006416837782340862
38	0.0	0.0	0.0	0.6160164271047228	0.006416837782340862
39	0.0	0.0	0.0	0.7379363449691991	0.006416837782340862
40	0.0	0.0	0.0	0.8662731006160165	0.006416837782340862
41	0.0	0.0	0.0	1.0587782340862424	0.006416837782340862
42	0.0	0.0	0.0	1.251283367556468	0.006416837782340862
43	0.0	0.0	0.0	1.4309548254620124	0.006416837782340862
44	0.0	0.0	0.0	1.6876283367556468	0.006416837782340862
45	0.0	0.0	0.0	1.7774640657084189	0.006416837782340862
46	0.0	0.0	0.0	1.9378850102669405	0.006416837782340862
47	0.0	0.0	0.0	2.1303901437371664	0.006416837782340862
48	0.0	0.0	0.0	2.284394250513347	0.006416837782340862
49	0.0	0.0	0.0	2.419147843942505	0.006416837782340862
50	0.0	0.0	0.0	2.5859856262833674	0.006416837782340862
51	0.0	0.0	0.0	2.797741273100616	0.006416837782340862
52	0.0	0.0	0.0	2.9324948665297743	0.006416837782340862
53	0.0	0.0	0.0	3.176334702258727	0.006416837782340862
54	0.0	0.0	0.0	3.413757700205339	0.006416837782340862
55	0.0	0.0	0.0	3.638347022587269	0.006416837782340862
56	0.0	0.0	0.0	3.824435318275154	0.006416837782340862
57	0.0	0.0	0.0	3.965605749486653	0.006416837782340862
58	0.0	0.0	0.0	4.100359342915811	0.006416837782340862
59	0.0	0.0	0.0	4.260780287474333	0.006416837782340862
60	0.0	0.0	0.0	4.382700205338809	0.006416837782340862
61	0.0	0.0	0.0	4.511036960985626	0.006416837782340862
62	0.0	0.0	0.0	4.581622176591376	0.006416837782340862
63	0.0	0.0	0.0	4.671457905544147	0.006416837782340862
64	0.0	0.0	0.0	4.729209445585216	0.006416837782340862
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTCCG	15	0.002183747	70.0	48
GTATCAT	15	0.002183747	70.0	57
TGCCTAC	20	7.685639E-5	70.0	19
AGTGTAG	20	7.685639E-5	70.0	35
GATCTCG	25	2.7113474E-6	70.0	41
CGTGTAT	15	0.002183747	70.0	35
CTGCCTA	15	0.002183747	70.0	18
ATCCGAC	15	0.002183747	70.0	10
AGATCTC	15	0.002183747	70.0	40
TCTCGGT	45	3.45608E-10	62.222218	43
ATCTCGG	35	2.788256E-7	60.000004	42
TCGGTGG	35	2.788256E-7	60.000004	45
CGCTTCC	35	2.788256E-7	60.000004	16
CGGTGGT	35	2.788256E-7	60.000004	46
CCGTATC	30	7.999513E-6	58.333332	55
TGTAGAT	30	7.999513E-6	58.333332	37
CGTATCA	25	2.3181946E-4	55.999996	56
TTACGCT	25	2.3181946E-4	55.999996	13
CTCGGTG	45	2.4791007E-8	54.444443	44
CTCTGCC	20	0.0068244096	52.5	16
>>END_MODULE