FastQCFastQC Report
Wed 25 May 2016
SRR1779764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15584
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT179811.537474332648872No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT4853.1121663244353184RNA PCR Primer, Index 47 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC2361.5143737166324436No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG2211.4181211498973307No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1951.251283367556468No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC1701.0908624229979467No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC820.5261806981519507No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT810.5197638603696099No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC790.5069301848049281No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG770.49409650924024645No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG770.49409650924024645No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC630.40426078028747436No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT600.3850102669404517No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT470.30159137577002054No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT350.22458932238193016No Hit
TCTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT290.186088295687885No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT260.1668377823408624No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGTCTT240.1540041067761807No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA200.12833675564681724No Hit
CTTATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC170.10908624229979465No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATA150.00218374770.02
TAATACA150.00218374770.04
TTAATAC150.00218374770.03
TCTTAAT150.00218374770.01
CTTTACA453.45608E-1062.2222181
TTACACA453.45608E-1062.2222183
TTTACAC453.45608E-1062.2222182
CTATACA352.788256E-760.0000041
AAAACTA200.006824409652.570
AATACAC200.006824409652.55
AAAAGAA351.993132E-550.00000470
AAACACA305.7013467E-446.66666470
ATCTCGT4750.041.2631637
TCTCGTA4750.041.2631638
CTTATAC4750.041.263161
CTCGTAT5100.040.49019239
GATCTCG4700.040.21276536
TGCTTGA5050.040.19802555
AAAAACC350.001217962840.00000466
TTATACA4900.040.02
Produced by FastQC (version 0.11.5)