##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779764_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15584
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.23941221765914	34.0	34.0	34.0	32.0	34.0
2	33.31371919917864	34.0	34.0	34.0	33.0	34.0
3	33.3758341889117	34.0	34.0	34.0	34.0	34.0
4	33.44808778234086	34.0	34.0	34.0	34.0	34.0
5	33.391042094455855	34.0	34.0	34.0	34.0	34.0
6	36.77354979466119	38.0	37.0	38.0	34.0	38.0
7	36.77996663244353	38.0	37.0	38.0	34.0	38.0
8	36.48966889117043	38.0	37.0	38.0	34.0	38.0
9	36.5444045174538	38.0	37.0	38.0	34.0	38.0
10-11	36.4577772073922	38.0	37.0	38.0	34.0	38.0
12-13	36.4227091889117	38.0	37.0	38.0	34.0	38.0
14-15	36.444654774127315	38.0	37.0	38.0	34.0	38.0
16-17	36.221188398357285	38.0	36.5	38.0	34.0	38.0
18-19	36.230524897330596	38.0	37.0	38.0	34.0	38.0
20-21	36.418955338809035	38.0	37.0	38.0	34.0	38.0
22-23	36.283656314168375	38.0	37.0	38.0	34.0	38.0
24-25	36.19285805954826	38.0	36.0	38.0	34.0	38.0
26-27	35.98350872689939	38.0	36.0	38.0	32.0	38.0
28-29	35.775250256673516	38.0	36.0	38.0	31.5	38.0
30-31	35.520501796714576	38.0	36.0	38.0	31.0	38.0
32-33	35.485465862423	38.0	36.0	38.0	31.0	38.0
34-35	35.38051848049281	38.0	36.0	38.0	29.0	38.0
36-37	35.355204055441476	38.0	36.0	38.0	31.0	38.0
38-39	34.42277335728953	37.0	34.0	38.0	26.0	38.0
40-41	35.38154517453799	37.5	36.0	38.0	29.5	38.0
42-43	35.850006416837786	38.0	36.0	38.0	31.5	38.0
44-45	35.86280800821355	38.0	36.0	38.0	31.0	38.0
46-47	35.834958932238195	38.0	36.0	38.0	31.5	38.0
48-49	35.81817890143738	38.0	36.0	38.0	31.5	38.0
50-51	35.709381416837786	38.0	36.0	38.0	31.0	38.0
52-53	35.75981776180698	38.0	36.0	38.0	31.0	38.0
54-55	35.67421714579056	38.0	36.0	38.0	31.0	38.0
56-57	35.63465734086242	38.0	36.0	38.0	31.0	38.0
58-59	35.463359856262834	38.0	36.0	38.0	31.0	38.0
60-61	35.49496278234086	38.0	36.0	38.0	31.0	38.0
62-63	35.53567761806981	38.0	36.0	38.0	31.0	38.0
64-65	35.609086242299796	38.0	36.0	38.0	31.0	38.0
66-67	35.55329183778234	38.0	36.0	38.0	31.0	38.0
68-69	34.8371727412731	38.0	35.0	38.0	26.0	38.0
70-71	33.0600295174538	37.5	31.5	38.0	17.0	38.0
72-73	30.73816093429158	37.0	24.0	38.0	11.0	38.0
74-75	30.342306211498972	37.0	23.0	38.0	11.0	38.0
76	29.748588295687885	36.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	0.0
22	4.0
23	8.0
24	16.0
25	38.0
26	51.0
27	114.0
28	150.0
29	240.0
30	404.0
31	550.0
32	848.0
33	1256.0
34	1991.0
35	2819.0
36	2755.0
37	4337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.678901437371664	17.26771047227926	13.135266940451745	38.91812114989733
2	16.43352156057495	14.899897330595483	45.61729979466119	23.049281314168375
3	15.663501026694046	21.817248459958932	41.24101642710472	21.2782340862423
4	12.833675564681723	28.343172484599588	31.02541067761807	27.79774127310062
5	12.403747433264888	34.59958932238193	39.502053388090346	13.494609856262835
6	24.287731006160165	41.40143737166325	15.368326488706366	18.942505133470224
7	22.56160164271047	30.44147843942505	16.06776180698152	30.92915811088296
8	19.71252566735113	41.63244353182752	16.401437371663246	22.253593429158112
9	19.147843942505133	17.94147843942505	14.22612936344969	48.684548254620125
10-11	21.201232032854207	27.204183778234086	27.61165297741273	23.982931211498972
12-13	21.358444558521562	15.807879876796715	28.789142710472284	34.04453285420945
14-15	22.568018480492814	17.04312114989733	17.729722792607802	42.659137577002056
16-17	30.547356262833674	26.92505133470226	15.111652977412732	27.415939425051334
18-19	30.27784907597536	21.258983572895275	16.20251540041068	32.26065195071869
20-21	22.240759753593426	18.67620636550308	15.669917864476385	43.413116016427104
22-23	22.946611909650922	28.19558521560575	15.740503080082135	33.11729979466119
24-25	31.176206365503077	29.693916837782343	15.541581108829568	23.58829568788501
26-27	29.748459958932237	32.834958932238195	16.65169404517454	20.76488706365503
28-29	22.381930184804926	31.558008213552363	18.637705338809035	27.422356262833674
30-31	24.01822381930185	28.461883983572893	27.486524640657084	20.033367556468175
32-33	38.98549794661191	20.22266427104723	21.80762320328542	18.984214579055443
34-35	26.001026694045176	27.938911704312115	27.566735112936346	18.493326488706366
36-37	29.03298254620123	28.391298767967143	21.461113963039015	21.114604722792606
38-39	21.778747433264886	19.73177618069815	28.19237679671458	30.29709958932238
40-41	20.97985112936345	17.521175564681723	29.806211498973305	31.69276180698152
42-43	30.030800821355236	17.87731006160164	29.957007186858313	22.134881930184804
44-45	22.372304928131417	25.29838295687885	30.30993326488706	22.01937885010267
46-47	29.68429158110883	17.48588295687885	22.045046201232033	30.784779260780287
48-49	29.36024127310062	15.429286447638605	21.249358316221766	33.96111396303901
50-51	21.41298767967146	16.074178644763858	30.80723819301848	31.7055954825462
52-53	19.64835728952772	16.154389117043124	39.98652464065708	24.210728952772072
54-55	20.055826488706366	16.038886036960985	33.065965092402465	30.839322381930184
56-57	28.90464579055442	16.1351386036961	24.83637063655031	30.123844969199176
58-59	21.653619096509242	19.9435318275154	36.20379876796714	22.199050308008214
60-61	27.560318275154007	31.596509240246405	21.563783367556468	19.279389117043124
62-63	18.95533880903491	44.090092402464066	17.447381930184804	19.50718685831622
64-65	18.08585728952772	46.522073921971256	16.3083932238193	19.083675564681723
66-67	17.32546201232033	46.54774127310062	16.15118069815195	19.975616016427107
68-69	17.800308008213552	45.559548254620125	16.488064681724847	20.15207905544148
70-71	17.947895277207394	43.05698151950719	17.755390143737166	21.239733059548254
72-73	19.3467659137577	36.15567248459959	19.43981006160164	25.057751540041068
74-75	19.892197125256676	33.54722792607803	20.726386036960985	25.834188911704313
76	20.37987679671458	34.74717659137577	20.046201232032853	24.826745379876797
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.0
15	1.0
16	1.5
17	1.0
18	2.5
19	5.0
20	8.0
21	10.0
22	17.0
23	23.0
24	27.5
25	33.0
26	45.0
27	56.5
28	61.0
29	66.0
30	88.5
31	114.5
32	132.0
33	146.0
34	184.5
35	231.0
36	256.0
37	273.0
38	364.0
39	939.0
40	1579.5
41	1611.0
42	1486.0
43	1272.5
44	922.0
45	667.5
46	550.0
47	501.5
48	444.0
49	384.0
50	333.0
51	322.0
52	273.5
53	236.5
54	237.0
55	247.5
56	238.5
57	191.0
58	163.0
59	176.0
60	182.0
61	215.0
62	255.0
63	239.5
64	230.0
65	266.5
66	287.5
67	278.0
68	282.0
69	312.0
70	313.0
71	288.0
72	280.0
73	275.5
74	258.0
75	237.0
76	216.5
77	179.0
78	145.0
79	128.0
80	99.5
81	64.5
82	47.0
83	36.0
84	29.0
85	17.0
86	9.5
87	7.0
88	4.5
89	1.5
90	2.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	15584.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.397587268993846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.50597337581068	43.71149897330596
2	15.917624303106157	17.954312114989733
3	3.9708726817612927	6.718429158110883
4	1.2970758903174424	2.9260780287474333
5	0.4664922061667994	1.3154517453798769
6	0.1706678803049266	0.5775154004106776
7	0.1251564455569462	0.49409650924024645
8	0.11377858686995107	0.5133470225872689
9	0.056889293434975535	0.2887577002053388
>10	0.22755717373990214	2.245893223819302
>50	0.07964501080896576	3.3303388090349078
>100	0.056889293434975535	8.386806981519507
>500	0.0	0.0
>1k	0.011377858686995108	11.537474332648872
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	1798	11.537474332648872	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	485	3.1121663244353184	RNA PCR Primer, Index 47 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	236	1.5143737166324436	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	221	1.4181211498973307	No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	195	1.251283367556468	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	170	1.0908624229979467	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	82	0.5261806981519507	No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT	81	0.5197638603696099	No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	79	0.5069301848049281	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	77	0.49409650924024645	No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	77	0.49409650924024645	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	63	0.40426078028747436	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT	60	0.3850102669404517	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT	47	0.30159137577002054	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT	35	0.22458932238193016	No Hit
TCTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	29	0.186088295687885	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT	26	0.1668377823408624	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGTCTT	24	0.1540041067761807	No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA	20	0.12833675564681724	No Hit
CTTATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	17	0.10908624229979465	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006416837782340862	0.0
2	0.0	0.0	0.0	0.006416837782340862	0.0
3	0.0	0.0	0.0	0.006416837782340862	0.0
4	0.0	0.0	0.0	0.006416837782340862	0.0
5	0.0	0.0	0.0	0.006416837782340862	0.0
6	0.0	0.0	0.0	0.006416837782340862	0.0
7	0.0	0.0	0.0	0.006416837782340862	0.0
8	0.0	0.0	0.0	0.006416837782340862	0.0
9	0.0	0.0	0.0	0.006416837782340862	0.0
10	0.0	0.0	0.0	0.019250513347022588	0.0
11	0.0	0.0	0.0	0.019250513347022588	0.0
12	0.0	0.0	0.0	0.019250513347022588	0.0
13	0.0	0.0	0.0	0.019250513347022588	0.0
14	0.0	0.0	0.0	0.019250513347022588	0.0
15	0.0	0.0	0.0	0.019250513347022588	0.0
16	0.0	0.0	0.0	0.019250513347022588	0.0
17	0.0	0.0	0.0	0.019250513347022588	0.0
18	0.0	0.0	0.0	0.019250513347022588	0.0
19	0.0	0.0	0.0	0.02566735112936345	0.0
20	0.0	0.0	0.0	0.038501026694045176	0.0
21	0.0	0.0	0.0	0.038501026694045176	0.0
22	0.0	0.0	0.0	0.038501026694045176	0.0
23	0.0	0.0	0.0	0.038501026694045176	0.0
24	0.0	0.0	0.0	0.038501026694045176	0.0
25	0.0	0.0	0.0	0.038501026694045176	0.0
26	0.0	0.0	0.0	0.038501026694045176	0.0
27	0.0	0.0	0.0	0.038501026694045176	0.0
28	0.0	0.0	0.0	0.04491786447638604	0.0
29	0.0	0.0	0.0	0.0513347022587269	0.0
30	0.0	0.0	0.0	0.06416837782340862	0.0
31	0.0	0.0	0.0	0.08983572895277207	0.0
32	0.0	0.0	0.0	0.09625256673511294	0.0
33	0.0	0.0	0.0	0.14117043121149897	0.0
34	0.0	0.0	0.0	0.24383983572895276	0.0
35	0.0	0.0	0.0	0.3400924024640657	0.0
36	0.0	0.0	0.0	0.4491786447638604	0.0
37	0.0	0.0	0.0	0.8085215605749486	0.0
38	0.0	0.0	0.0	1.0074435318275154	0.0
39	0.0	0.0	0.0	1.1678644763860369	0.0
40	0.0	0.0	0.0	1.3603696098562628	0.0
41	0.0	0.0	0.0	1.591375770020534	0.0
42	0.0	0.0	0.0	1.86088295687885	0.0
43	0.0	0.0	0.0	2.091889117043121	0.0
44	0.0	0.0	0.0	2.3934804928131417	0.0
45	0.0	0.006416837782340862	0.0	2.6180698151950716	0.0
46	0.0	0.006416837782340862	0.0	2.9196611909650922	0.0
47	0.0	0.006416837782340862	0.0	3.1635010266940453	0.0
48	0.0	0.006416837782340862	0.0	3.4265913757700206	0.0
49	0.0	0.006416837782340862	0.0	3.6768480492813143	0.0
50	0.0	0.006416837782340862	0.0	3.8886036960985626	0.0
51	0.0	0.006416837782340862	0.0	4.126026694045175	0.0
52	0.0	0.006416837782340862	0.0	4.305698151950718	0.0
53	0.0	0.006416837782340862	0.0	4.530287474332649	0.0
54	0.0	0.006416837782340862	0.0	4.76129363449692	0.0
55	0.0	0.006416837782340862	0.0	5.082135523613963	0.0
56	0.0	0.006416837782340862	0.0	5.325975359342916	0.0
57	0.0	0.006416837782340862	0.0	5.589065708418891	0.0
58	0.0	0.006416837782340862	0.0	5.813655030800821	0.0
59	0.0	0.006416837782340862	0.0	6.0767453798767965	0.0
60	0.0	0.006416837782340862	0.0	6.314168377823409	0.0
61	0.0	0.006416837782340862	0.0	6.615759753593429	0.0
62	0.0	0.006416837782340862	0.0	6.8467659137577	0.0
63	0.0	0.006416837782340862	0.0	7.058521560574949	0.0
64	0.0	0.006416837782340862	0.0	7.308778234086242	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAATA	15	0.002183747	70.0	2
TAATACA	15	0.002183747	70.0	4
TTAATAC	15	0.002183747	70.0	3
TCTTAAT	15	0.002183747	70.0	1
CTTTACA	45	3.45608E-10	62.222218	1
TTACACA	45	3.45608E-10	62.222218	3
TTTACAC	45	3.45608E-10	62.222218	2
CTATACA	35	2.788256E-7	60.000004	1
AAAACTA	20	0.0068244096	52.5	70
AATACAC	20	0.0068244096	52.5	5
AAAAGAA	35	1.993132E-5	50.000004	70
AAACACA	30	5.7013467E-4	46.666664	70
ATCTCGT	475	0.0	41.26316	37
TCTCGTA	475	0.0	41.26316	38
CTTATAC	475	0.0	41.26316	1
CTCGTAT	510	0.0	40.490192	39
GATCTCG	470	0.0	40.212765	36
TGCTTGA	505	0.0	40.198025	55
AAAAACC	35	0.0012179628	40.000004	66
TTATACA	490	0.0	40.0	2
>>END_MODULE