##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779763_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16542
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.040805223068553	32.0	21.0	34.0	12.0	34.0
2	26.341977995405635	31.0	21.0	34.0	12.0	34.0
3	25.79839197195019	31.0	21.0	34.0	11.0	34.0
4	26.999879095635354	32.0	21.0	34.0	12.0	34.0
5	26.91083303107242	32.0	23.0	34.0	11.0	34.0
6	28.68764357393302	34.0	21.0	38.0	11.0	38.0
7	29.65953330915246	34.0	22.0	38.0	12.0	38.0
8	29.08324265505985	34.0	21.0	38.0	11.0	38.0
9	30.461189698948132	36.0	26.0	38.0	12.0	38.0
10-11	29.80561600773788	36.0	23.0	38.0	11.0	38.0
12-13	29.399679603433682	35.5	23.0	38.0	11.0	38.0
14-15	28.873413130214	34.0	21.0	38.0	11.0	38.0
16-17	28.883871357756014	34.5	22.0	38.0	11.0	38.0
18-19	29.32459799298755	34.0	22.5	38.0	11.0	38.0
20-21	28.446439366461128	32.5	21.5	37.5	11.0	38.0
22-23	28.416606214484343	33.5	17.0	37.5	11.0	38.0
24-25	29.28439729174223	35.0	22.0	38.0	11.0	38.0
26-27	27.975123926973765	32.5	21.0	38.0	11.0	38.0
28-29	29.518951759158504	36.0	21.5	38.0	11.0	38.0
30-31	30.03645266594124	37.0	22.5	38.0	11.0	38.0
32-33	30.083877402974245	37.0	22.5	38.0	11.0	38.0
34-35	30.16866158868335	37.0	23.0	38.0	11.0	38.0
36-37	28.71656994317495	34.0	22.0	38.0	11.0	38.0
38-39	27.68126586869786	31.5	21.0	37.0	11.0	38.0
40-41	28.06601378309757	33.5	21.0	37.5	11.0	38.0
42-43	28.895689759400312	35.0	21.5	38.0	11.0	38.0
44-45	30.191603191875227	37.0	23.5	38.0	11.0	38.0
46-47	29.12755410470318	35.5	22.0	38.0	11.0	38.0
48-49	28.388647080159593	33.5	16.5	38.0	11.0	38.0
50-51	26.883720227300206	31.0	16.0	37.0	11.0	38.0
52-53	29.22219199613106	35.0	21.5	38.0	11.0	38.0
54-55	28.618818764357393	34.5	21.0	38.0	11.0	38.0
56-57	29.021974368274698	35.5	22.0	38.0	11.0	38.0
58-59	29.767833393785516	36.5	22.0	38.0	11.0	38.0
60-61	30.22267561358965	37.0	23.0	38.0	11.0	38.0
62-63	30.237395719985493	37.0	23.0	38.0	11.0	38.0
64-65	29.977239753355096	37.0	22.5	38.0	11.0	38.0
66-67	28.565681296094787	34.0	21.0	38.0	11.0	38.0
68-69	28.528896143150767	34.0	21.0	38.0	11.0	38.0
70-71	28.045822754201428	34.0	21.0	38.0	11.0	38.0
72-73	27.2339801716842	31.5	16.0	37.0	11.0	38.0
74-75	28.439970982952484	35.0	21.0	37.5	11.0	38.0
76	28.72300810059243	35.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	8.0
13	68.0
14	188.0
15	316.0
16	492.0
17	779.0
18	873.0
19	892.0
20	736.0
21	579.0
22	441.0
23	297.0
24	296.0
25	248.0
26	219.0
27	212.0
28	258.0
29	255.0
30	323.0
31	504.0
32	667.0
33	988.0
34	1436.0
35	2012.0
36	2247.0
37	1206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.70443141285291	19.128226830300466	13.729520585212502	38.43782117163413
2	15.935195260548907	14.514568975940032	48.43428847781405	21.115947285697015
3	18.171926006528835	15.693386531253777	42.23189457139403	23.90279289082336
4	12.821907870874139	21.629790835449157	31.88248095756257	33.66582033611413
5	12.75541047031798	24.990932172651434	44.22077136984645	18.032885987184137
6	23.00205537419901	35.73328497158747	16.539717083786726	24.72494257042679
7	21.62374561721678	24.918389553862895	20.49328980776206	32.96457502115827
8	18.347237335267803	33.635594244952244	17.839439003748037	30.177729416031916
9	19.526054890581552	14.937734252206505	19.042437431991292	46.49377342522065
10-11	20.979929875468503	23.8634989723129	28.554588320638373	26.601982831580216
12-13	20.505380244226817	17.812235521702334	32.490025389916575	29.192358844154278
14-15	22.64236488937251	22.470076169749728	24.02369725547092	30.863861685406846
16-17	26.051867972433808	20.65651070003627	24.945593035908598	28.346028291621327
18-19	19.831338411316647	20.209164550840285	30.24120420747189	29.71829283037118
20-21	25.837262725184377	19.571394027324388	25.761697497279652	28.82964575021158
22-23	26.93144722524483	18.773425220650466	24.95768347237335	29.337444081731352
24-25	20.63232982710676	24.709829524845848	22.63027445290775	32.02756619513964
26-27	24.585902551082096	23.135050175311328	26.045822754201424	26.23322451940515
28-29	22.068069157296577	23.787933744408175	25.371780921291258	28.77221617700399
30-31	21.505863861685405	23.346632813444565	26.611050658928786	28.53645266594124
32-33	21.602587353403457	22.950671019223794	26.45992020311933	28.986821424253417
34-35	21.629790835449157	23.225728448797	26.00652883569097	29.137951880062868
36-37	26.184862773546126	19.444444444444446	30.552532946439364	23.818159835570064
38-39	25.29923830250272	17.567404183291018	35.03808487486398	22.09527263934228
40-41	23.334542376979808	24.035787691935678	24.740055616007737	27.88961431507677
42-43	18.42280256317253	24.66449038810301	27.532946439366462	29.379760609357998
44-45	18.812719139160926	20.756256800870514	30.45278684560513	29.97823721436344
46-47	24.652399951638255	19.65300447346149	27.925885624471043	27.76870995042921
48-49	24.649377342522065	20.25148107846693	29.57925281102648	25.519888767984522
50-51	31.906661830492084	17.92709466811752	27.678031676943537	22.488211824446864
52-53	19.093821786966508	22.013662193205175	29.618546729536938	29.27396929029138
54-55	24.241325111836538	20.444928061903035	27.36065771974368	27.953089106516742
56-57	23.99951638254141	20.65046548180389	26.03977753596905	29.31024059968565
58-59	19.32051747068069	23.06855277475517	28.90521097811631	28.70571877644783
60-61	18.52859388223915	21.327529923830248	29.074477088622903	31.0693991053077
62-63	18.564865191633416	22.986942328618063	28.27650828194898	30.171684197799543
64-65	19.06359569580462	24.885140853584815	26.952605489058158	29.09865796155241
66-67	17.198645871115946	28.7691935678878	21.871599564744287	32.16056099625197
68-69	17.981501632208925	28.524362229476484	21.92902913795188	31.565107000362712
70-71	18.371418208197316	29.594365856607425	21.112924676580825	30.921291258614435
72-73	17.86059726756136	30.192842461612862	20.472131543948738	31.47442872687704
74-75	19.601620118486277	25.90980534397292	23.603554588320637	30.885019949220165
76	20.40261153427639	24.168782493048	25.6377705235159	29.790835449159715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.0
15	1.0
16	4.0
17	6.0
18	5.0
19	6.5
20	10.5
21	12.0
22	14.0
23	27.0
24	40.0
25	42.0
26	46.5
27	70.5
28	106.5
29	123.0
30	142.0
31	166.0
32	205.5
33	240.0
34	273.0
35	335.0
36	379.5
37	395.0
38	481.5
39	591.5
40	646.5
41	713.5
42	749.0
43	746.0
44	781.5
45	833.0
46	846.0
47	837.5
48	809.0
49	754.5
50	720.0
51	697.5
52	625.5
53	564.0
54	552.0
55	516.0
56	475.5
57	443.0
58	415.0
59	371.5
60	331.5
61	326.0
62	317.0
63	322.5
64	313.0
65	314.0
66	309.0
67	288.0
68	291.0
69	278.5
70	246.0
71	229.0
72	228.0
73	212.0
74	177.5
75	158.0
76	136.0
77	103.0
78	85.5
79	79.0
80	59.0
81	39.0
82	26.5
83	14.0
84	11.0
85	6.0
86	3.5
87	3.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0060452182323781885
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	16542.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.20203119332608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.25045703839123	98.45846935074356
2	0.7068860450944546	1.4024906299117397
3	0.030469226081657527	0.09067827348567283
4	0.012187690432663009	0.04836174585902551
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0060452182323781885	0.0
21	0.0	0.0	0.0	0.0060452182323781885	0.0
22	0.0	0.0	0.0	0.06045218232378189	0.0
23	0.0	0.0	0.0	0.06045218232378189	0.0
24	0.0	0.0	0.0	0.1088139281828074	0.0
25	0.0	0.0	0.0	0.1088139281828074	0.0
26	0.0	0.0	0.0	0.1088139281828074	0.0
27	0.0	0.0	0.0	0.11485914641518559	0.0
28	0.0	0.0	0.0	0.13299480111232015	0.0
29	0.0	0.0	0.0	0.13904001934469834	0.0
30	0.0	0.0	0.0	0.1571756740418329	0.0
31	0.0	0.0	0.0	0.1571756740418329	0.0
32	0.0	0.0	0.0	0.18135654697134568	0.0
33	0.0	0.0	0.0	0.18740176520372384	0.0
34	0.0	0.0	0.0	0.19344698343610203	0.0
35	0.0	0.0	0.0	0.2659896022246403	0.0
36	0.0	0.0	0.0	0.29621569338653125	0.0
37	0.0	0.0	0.0	0.45339136742836417	0.0
38	0.0	0.0	0.0	0.5863861685406844	0.0
39	0.0	0.0	0.0	0.7254261878853827	0.0
40	0.0	0.0	0.0	0.8765566436948374	0.0
41	0.0	0.0	0.0	1.0700036271309394	0.0
42	0.0	0.0	0.0	1.215088864708016	0.0
43	0.0	0.0	0.0	1.40249062991174	0.0
44	0.0	0.0	0.0	1.6866158868335146	0.0
45	0.0	0.0	0.0	1.8377463426429694	0.0
46	0.0	0.0	0.0	2.049328980776206	0.0
47	0.0	0.0	0.0	2.212549873050417	0.0
48	0.0	0.0	0.0	2.442268165880788	0.0
49	0.0	0.0	0.0	2.593398621690243	0.0
50	0.0	0.0	0.0	2.786845605126345	0.0
51	0.0	0.0	0.0	3.034699552653851	0.0
52	0.0	0.0	0.0	3.330915246040382	0.0
53	0.0	0.0	0.0	3.5969048482650225	0.0
54	0.0	0.0	0.0	3.814532704630637	0.0
55	0.0	0.0	0.0	4.026115342763874	0.0
56	0.0	0.0	0.0	4.2497884173618665	0.0
57	0.0	0.0	0.0	4.419054527868456	0.0
58	0.0	0.0	0.0	4.570184983677911	0.0
59	0.0	0.0	0.0	4.757586748881635	0.0
60	0.0	0.0	0.0	4.902671986458711	0.0
61	0.0	0.0	0.0	4.9873050417120055	0.0
62	0.0	0.0	0.0	5.0598476605005445	0.0
63	0.0	0.0	0.0	5.186797243380486	0.0
64	0.0	0.0	0.0	5.30770160802805	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTACGA	15	0.0021861359	70.00001	21
ATCTCGG	15	0.0021861359	70.00001	42
CTTTACG	15	0.0021861359	70.00001	30
GTGTATC	15	0.0021861359	70.00001	36
GTCGCCC	15	0.0021861359	70.00001	57
CCTCTTA	15	0.0021861359	70.00001	9
ACGAGCC	15	0.0021861359	70.00001	24
CGTATCA	15	0.0021861359	70.00001	56
CGACGAC	15	0.0021861359	70.00001	22
AGATCTC	15	0.0021861359	70.00001	40
ACGTGTC	15	0.0021861359	70.00001	34
ACGCTTC	25	2.322404E-4	55.999996	15
TCGGTGG	25	2.322404E-4	55.999996	45
CTCGGTG	25	2.322404E-4	55.999996	44
TCCTTAA	20	0.0068318197	52.500004	60
TGTCTTA	20	0.0068318197	52.500004	56
CACGTGT	20	0.0068318197	52.500004	33
GCGGTCG	20	0.0068318197	52.500004	48
CCGTATC	20	0.0068318197	52.500004	55
GACGCTT	20	0.0068318197	52.500004	14
>>END_MODULE