FastQCFastQC Report
Wed 25 May 2016
SRR1779763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16542
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT226613.698464514568975No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT5223.1556039173014145Illumina PCR Primer Index 9 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG3241.958650707290533No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2661.6080280498125983No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2611.5778019586507073No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2001.2090436464756378No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT1070.6468383508644662Illumina PCR Primer Index 9 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC990.5984766050054406No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG940.5682505138435497No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG910.5501148591464152No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC890.5380244226816588No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT820.49570789505501145No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT570.3445774392455568No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT530.320396566316044No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC490.29621569338653125No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT360.2176278563656148No Hit
TCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT330.19949220166848025No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCTT250.15113045580945472No Hit
CTTATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC230.13904001934469834No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG220.13299480111232015No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG220.13299480111232015No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGTC200.12090436464756377No Hit
CTTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC180.1088139281828074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA501.2732926E-1162.9999961
TCTTAAT200.006831819752.5000041
TCTTTAT351.9985508E-550.01
CTTATAC4350.044.2528761
TTATACA4750.040.5263142
CTTTATA458.813685E-538.888892
AGATCTC4650.037.6344135
TCTCGTA4750.037.57894538
CAGATCT4700.037.23404334
ATCTCGT4800.037.18750437
TATACAC5300.036.9811333
GATCTCG4750.036.84210236
AACAGAT4850.036.80412332
GAAAAAA5050.036.73267460
TGCTTGA5150.036.69902855
GCTTGAA5150.036.69902856
CTTGAAA5150.036.69902857
CCGTCTT5250.036.66666847
GTCTTCT5250.036.66666849
CTGCTTG5250.036.66666854
Produced by FastQC (version 0.11.5)