##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779762_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55999
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.562242182896124	34.0	27.0	34.0	12.0	34.0
2	27.873140591796282	34.0	23.0	34.0	12.0	34.0
3	26.992482008607297	32.0	23.0	34.0	11.0	34.0
4	29.411507348345506	34.0	24.0	34.0	12.0	34.0
5	28.830996982088966	34.0	23.0	34.0	11.0	34.0
6	31.359613564527937	37.0	27.0	38.0	11.0	38.0
7	32.497866033322026	37.0	31.0	38.0	12.0	38.0
8	31.643797210664477	37.0	27.0	38.0	11.0	38.0
9	33.1617350309827	37.0	34.0	38.0	21.0	38.0
10-11	32.85789031946999	37.5	33.0	38.0	11.0	38.0
12-13	31.84877408525152	37.0	29.5	38.0	16.0	38.0
14-15	31.85919391417704	37.0	29.0	38.0	11.5	38.0
16-17	31.56078679976428	36.5	28.5	38.0	16.0	38.0
18-19	32.01302701833961	37.0	29.0	38.0	11.5	38.0
20-21	31.07127805853676	36.0	27.0	38.0	11.0	38.0
22-23	30.97053518812836	35.5	27.5	38.0	11.0	38.0
24-25	32.30960374292398	37.0	30.0	38.0	11.0	38.0
26-27	30.680181788960518	36.5	25.0	38.0	11.0	38.0
28-29	32.93039161413597	37.0	33.0	38.0	11.0	38.0
30-31	33.38960517152092	38.0	34.0	38.0	16.0	38.0
32-33	33.50180360363578	38.0	34.0	38.0	16.0	38.0
34-35	33.57058161752889	38.0	34.0	38.0	21.0	38.0
36-37	31.29719280701441	36.5	27.5	38.0	11.0	38.0
38-39	29.495660636797087	34.5	23.0	38.0	11.0	38.0
40-41	30.812068072644156	35.5	26.0	38.0	11.0	38.0
42-43	31.919953927748708	36.5	30.0	38.0	16.0	38.0
44-45	33.5950820550367	38.0	34.0	38.0	21.0	38.0
46-47	32.07396560652869	37.5	29.5	38.0	16.0	38.0
48-49	31.321318237825672	36.5	27.5	38.0	14.0	38.0
50-51	29.175547777638887	34.5	22.5	38.0	11.0	38.0
52-53	32.71332523795068	37.0	32.5	38.0	15.5	38.0
54-55	31.761576099573205	37.0	29.0	38.0	11.0	38.0
56-57	32.17600314291327	37.5	29.5	38.0	16.0	38.0
58-59	33.34552402721477	37.5	34.0	38.0	21.0	38.0
60-61	33.82626475472776	38.0	35.0	38.0	21.0	38.0
62-63	33.706655475990644	38.0	34.5	38.0	16.0	38.0
64-65	33.36294398114252	38.0	35.0	38.0	16.0	38.0
66-67	31.394551688423007	37.0	26.5	38.0	11.0	38.0
68-69	31.381890748049074	37.0	26.5	38.0	11.0	38.0
70-71	30.772147716923516	36.5	25.5	38.0	11.0	38.0
72-73	29.653797389239095	35.0	23.5	38.0	11.0	38.0
74-75	31.75641529313023	36.5	29.0	38.0	11.0	38.0
76	32.37006017964607	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	21.0
13	113.0
14	302.0
15	482.0
16	729.0
17	1097.0
18	1361.0
19	1427.0
20	1165.0
21	949.0
22	781.0
23	679.0
24	631.0
25	597.0
26	706.0
27	764.0
28	1015.0
29	1205.0
30	1709.0
31	2297.0
32	3154.0
33	4660.0
34	6406.0
35	8859.0
36	9926.0
37	4964.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.72922730763049	18.562831479133557	11.348416936016715	33.359524277219236
2	20.721798603546493	16.43422203967928	39.17034232754156	23.673637029232665
3	19.786067608350148	16.92173074519188	36.14885980106788	27.143341845390097
4	14.689810350369656	18.01850066073788	31.131468981035038	36.160220007857426
5	14.769906605475096	28.45943677565671	39.19712852015215	17.57352809871605
6	29.427311201985752	32.2309326952267	15.930641618600333	22.411114484187216
7	27.355845640100718	25.812960945731174	19.400346434757765	27.430846979410344
8	23.291487347988358	31.350559831425564	18.109251950927696	27.24870086965839
9	22.359327845140093	14.987767638707833	19.628921945034733	43.023982571117344
10-11	25.240629296951735	23.024518294969553	26.36207789424811	25.372774513830603
12-13	24.28614796692798	18.91640922159324	28.64961874319184	28.147824068286937
14-15	23.987035482776477	21.703958999267844	23.4405971535206	30.86840836443508
16-17	25.43884712227004	22.156645654386686	25.47634779192486	26.92815943141842
18-19	22.599510705548315	22.10396614225254	27.390667690494475	27.90585546170467
20-21	24.89330166610118	21.74056679583564	26.18171753067019	27.184414007392988
22-23	25.689744459722498	21.172699512491295	26.374578117466385	26.76297791031983
24-25	22.953088448008	24.263826139752496	23.255772424507583	29.527312987731925
26-27	23.872747727637993	23.24595082055037	26.553152734870267	26.328148716941374
28-29	23.6522080751442	24.132573795960642	25.16384221146806	27.051375917427094
30-31	23.697744602582187	23.896855300987518	24.97008875158485	27.43531134484544
32-33	23.820961088590867	23.67720852158074	25.17991392703441	27.321916462793975
34-35	23.34505973320952	23.675422775406705	25.419203914355613	27.56031357702816
36-37	24.737941748959802	21.381631814853836	29.220164645797247	24.660261790389114
38-39	24.59240343577564	20.26821907534063	32.42111466276184	22.718262826121897
40-41	23.87810496616011	23.086126537973893	24.22543259701066	28.81033589885534
42-43	21.563777924605795	23.596849943748996	26.146895480276434	28.692476651368775
44-45	21.890569474454903	22.28254075965642	27.711209128734442	28.115680637154234
46-47	23.798851754957724	21.235390233666973	28.088252363902605	26.8775056474727
48-49	24.276326362970767	21.477169235164915	28.390685547956213	25.855818853908108
50-51	25.94064179717495	20.331613064519008	29.979999642850764	23.747745495455273
52-53	21.21109305523313	22.716477079947854	28.012107359059986	28.060322505759032
54-55	23.382560402864335	21.811103769710176	27.411203771495916	27.395132055929572
56-57	23.166485115805642	22.54325970106609	26.53350952695584	27.756745656172434
58-59	21.067340488223003	23.505776888873015	27.44066858336756	27.98621403953642
60-61	21.110198396399934	22.634332755942072	27.402275040625725	28.85319380703227
62-63	20.657511741281095	23.232557724245076	27.312987731923783	28.796942802550046
64-65	21.306630475544207	24.078108537652458	26.252254504544727	28.363006482258612
66-67	20.21018232468437	24.4986517616386	23.24059358202825	32.05057233164878
68-69	20.64565100910877	24.76245758171102	23.05679585640293	31.535095552777282
70-71	20.666440472151287	25.13973463811854	22.512902016107432	31.68092287362274
72-73	20.900373220950375	24.77365667244058	21.31466633332738	33.01130377328166
74-75	22.299505348309793	24.327220128930875	23.806675119198555	29.56659940356078
76	23.712923445061517	23.128984446150824	24.43615064554724	28.721941463240412
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.5
16	4.5
17	6.0
18	7.0
19	8.0
20	11.0
21	14.0
22	27.0
23	47.5
24	63.5
25	72.0
26	101.0
27	162.5
28	229.5
29	264.0
30	333.5
31	438.5
32	586.5
33	699.0
34	772.5
35	957.5
36	1224.5
37	1380.0
38	1525.5
39	1786.0
40	2072.5
41	2342.5
42	2441.0
43	2555.5
44	2707.0
45	2771.5
46	2799.0
47	2724.5
48	2641.5
49	2563.0
50	2493.0
51	2369.5
52	2144.5
53	1944.5
54	1846.0
55	1781.5
56	1683.5
57	1615.5
58	1581.0
59	1546.5
60	1505.5
61	1472.5
62	1446.0
63	1443.5
64	1417.5
65	1366.0
66	1252.0
67	1166.0
68	1125.0
69	997.0
70	848.5
71	787.0
72	686.0
73	530.0
74	440.0
75	405.0
76	345.0
77	260.5
78	200.5
79	165.0
80	141.0
81	94.5
82	52.0
83	32.0
84	25.5
85	16.0
86	10.0
87	7.0
88	6.5
89	4.0
90	2.0
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0017857461740388224
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	8.928730870194112E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0160717155663494
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	55999.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.88389078376399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.22308169445762	96.14457401025018
2	1.488670777538585	2.9143377560313577
3	0.21892217316743898	0.6428686226539759
4	0.04925748896267377	0.19286058679619278
5	0.014594811544495934	0.07142984696155288
6	0.0036487028861239834	0.021428954088465863
7	0.0018243514430619917	0.012500223218271756
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0017857461740388221	0.0
10	0.0	0.0	0.0	0.0017857461740388221	0.0
11	0.0	0.0	0.0	0.0017857461740388221	0.0
12	0.0	0.0	0.0	0.0017857461740388221	0.0
13	0.0	0.0	0.0	0.0017857461740388221	0.0
14	0.0	0.0	0.0	0.0017857461740388221	0.0
15	0.0	0.0	0.0	0.0017857461740388221	0.0
16	0.0	0.0	0.0	0.0035714923480776443	0.0
17	0.0	0.0	0.0	0.0035714923480776443	0.0
18	0.0	0.0	0.0	0.0035714923480776443	0.0
19	0.0	0.0	0.0	0.007142984696155289	0.0
20	0.0	0.0	0.0	0.00892873087019411	0.0
21	0.0	0.0	0.0	0.00892873087019411	0.0
22	0.0	0.0	0.0	0.01785746174038822	0.0
23	0.0	0.0	0.0	0.02500044643654351	0.0
24	0.0	0.0	0.0	0.037500669654815265	0.0
25	0.0	0.0	0.0	0.03928641582885409	0.0
26	0.0	0.0	0.0	0.044643654350970556	0.0
27	0.0	0.0	0.0	0.06250111609135878	0.0
28	0.0	0.0	0.0	0.08393007017982464	0.0
29	0.0	0.0	0.0	0.09285880105001874	0.0
30	0.0	0.0	0.0	0.10535902426829051	0.0
31	0.0	0.0	0.0	0.11428775513848462	0.0
32	0.0	0.0	0.0	0.13393096305291166	0.0
33	0.0	0.0	0.0	0.14821693244522224	0.0
34	0.0	0.0	0.0	0.17500312505580456	0.0
35	0.0	0.0	0.0	0.24107573349524097	0.0
36	0.0	0.0	0.0	0.3160770728048715	0.0
37	0.0	0.0	0.0	0.532152359863569	0.0017857461740388221
38	0.0	0.0	0.0	0.7035839925712959	0.0017857461740388221
39	0.0	0.0	0.0	0.9303737566742263	0.0017857461740388221
40	0.0	0.0	0.0	1.203592921302166	0.0017857461740388221
41	0.0	0.0	0.0	1.5589564099358917	0.0017857461740388221
42	0.0	0.0	0.0	1.9482490758763549	0.0017857461740388221
43	0.0	0.0	0.0	2.3875426346899054	0.0017857461740388221
44	0.0	0.0	0.0	2.8125502241111446	0.0017857461740388221
45	0.0	0.0	0.0	3.1286272969160165	0.0017857461740388221
46	0.0	0.0	0.0	3.510776978160324	0.0017857461740388221
47	0.0	0.0	0.0	4.021500383935427	0.0017857461740388221
48	0.0	0.0	0.0	4.359006410828765	0.0017857461740388221
49	0.0	0.0	0.0	4.684012214503831	0.0017857461740388221
50	0.0	0.0	0.0	5.180449650886623	0.0017857461740388221
51	0.0	0.0	0.0	5.703673279879998	0.0017857461740388221
52	0.0	0.0	0.0	6.150109823389704	0.0017857461740388221
53	0.0	0.0	0.0	6.7501205378667475	0.0017857461740388221
54	0.0	0.0	0.0	7.296558867122627	0.0017857461740388221
55	0.0	0.0	0.0	7.692994517759246	0.0017857461740388221
56	0.0	0.0	0.0	8.105501883962214	0.0017857461740388221
57	0.0	0.0	0.0	8.507294773120949	0.0017857461740388221
58	0.0	0.0	0.0	8.80730013035947	0.0017857461740388221
59	0.0	0.0	0.0	9.200164288648011	0.0017857461740388221
60	0.0	0.0	0.0	9.444811514491331	0.0017857461740388221
61	0.0	0.0	0.0	9.70195896355292	0.0017857461740388221
62	0.0	0.0	0.0	9.876962088608725	0.0017857461740388221
63	0.0	0.0	0.0	10.130538045322238	0.0017857461740388221
64	0.0	0.0	0.0	10.366256540295362	0.0017857461740388221
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACACT	15	0.0022146134	69.993744	38
TCATTAA	30	1.0071926E-7	69.993744	60
CAATTGT	15	0.0022146134	69.993744	14
ACGCTTC	30	8.281186E-6	58.32812	15
CGAAACT	25	2.3724108E-4	55.99499	25
CGGTTGT	25	2.3724108E-4	55.99499	46
TTCGTTT	25	2.3724108E-4	55.99499	33
CTCGGTT	25	2.3724108E-4	55.99499	44
TCGGTTG	25	2.3724108E-4	55.99499	45
CGTATCC	20	0.006920138	52.49531	56
GAAACTC	20	0.006920138	52.49531	26
CGTCCAT	20	0.006920138	52.49531	37
GCGTCGC	20	0.006920138	52.49531	36
TCGGTGT	20	0.006920138	52.49531	45
TGGTCTC	20	0.006920138	52.49531	49
GCACTAA	20	0.006920138	52.49531	28
CAGACTT	20	0.006920138	52.49531	14
CATCTCA	35	2.0629644E-5	49.995533	9
CGGTGTT	35	2.0629644E-5	49.995533	46
GCCTACG	35	2.0629644E-5	49.995533	20
>>END_MODULE