FastQCFastQC Report
Wed 25 May 2016
SRR1779762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55999
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT36716.555474204896516No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT5881.0500187503348273RNA PCR Primer, Index 42 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG4880.8714441329309451No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3760.6714405614385971RNA PCR Primer, Index 32 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC3100.5535813139520349No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2440.43572206646547257No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1550.27679065697601746No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT1430.25536170288755156No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1400.2500044643654351RNA PCR Primer, Index 32 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1330.23750424114716337No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1150.20536081001446455No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT980.17500312505580456RNA PCR Primer, Index 42 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG970.17321737888176575No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT780.13928820157502814No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT730.13035947070483403No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT610.10893051661636814No Hit
TCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT560.10000178574617405No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATTC150.002213828570.016
TTCCATC150.002213828570.03
TTCGGCT150.002213828570.020
TCTTTAT453.7471182E-1062.2222251
TTAATAC252.3713624E-456.0000042
TTTACAC252.3713624E-456.0000042
CTTTATA509.531504E-1056.0000042
TGTGGAA200.006917693752.549
ATTCGGC200.006917693752.519
TCGGCTA200.006917693752.521
GAGACGT200.006917693752.524
TATTCGG200.006917693752.518
CGGCTAT200.006917693752.522
CCGAAAA200.006917693752.527
ACCCGAT200.006917693752.532
TTCGAAC200.006917693752.557
ACTCGAC451.6570011E-646.66666832
CGAACTC305.8313156E-446.66666435
AACTCGT305.8313156E-446.66666437
AATACAC404.5387285E-543.754