##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779762_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55999
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.30541616814586	34.0	34.0	34.0	33.0	34.0
2	33.32488080144288	34.0	34.0	34.0	33.0	34.0
3	33.344220432507726	34.0	34.0	34.0	33.0	34.0
4	33.44145431168414	34.0	34.0	34.0	34.0	34.0
5	33.38022107537635	34.0	34.0	34.0	34.0	34.0
6	36.679922855765284	38.0	37.0	38.0	34.0	38.0
7	36.76035286344399	38.0	37.0	38.0	34.0	38.0
8	36.43463276129931	38.0	37.0	38.0	34.0	38.0
9	36.533723816496725	38.0	37.0	38.0	34.0	38.0
10-11	36.40490008750156	38.0	37.0	38.0	34.0	38.0
12-13	36.40326612975232	38.0	37.0	38.0	34.0	38.0
14-15	36.462463615421704	38.0	37.0	38.0	34.0	38.0
16-17	36.33105948320505	38.0	37.0	38.0	34.0	38.0
18-19	36.33734530973767	38.0	37.0	38.0	34.0	38.0
20-21	36.41400739298916	38.0	37.0	38.0	34.0	38.0
22-23	36.27771031625565	38.0	37.0	38.0	34.0	38.0
24-25	36.23275415632422	38.0	37.0	38.0	34.0	38.0
26-27	36.04952767013697	38.0	36.5	38.0	32.5	38.0
28-29	35.897980321077156	38.0	36.0	38.0	31.5	38.0
30-31	35.7346470472687	38.0	36.0	38.0	31.0	38.0
32-33	35.69430704119716	38.0	36.0	38.0	31.0	38.0
34-35	35.562768978017466	38.0	36.0	38.0	31.0	38.0
36-37	35.715780638939975	38.0	36.0	38.0	31.0	38.0
38-39	35.165101162520756	38.0	36.0	38.0	27.0	38.0
40-41	35.71468240504295	38.0	36.0	38.0	31.5	38.0
42-43	35.935204200075006	38.0	36.5	38.0	32.0	38.0
44-45	35.93795424918302	38.0	36.0	38.0	32.0	38.0
46-47	35.972401292880235	38.0	37.0	38.0	32.0	38.0
48-49	35.96800835729209	38.0	36.5	38.0	32.0	38.0
50-51	35.89793567742281	38.0	36.0	38.0	32.0	38.0
52-53	35.913668101216096	38.0	37.0	38.0	32.0	38.0
54-55	35.86799764281505	38.0	37.0	38.0	32.0	38.0
56-57	35.84258647475848	38.0	36.0	38.0	31.5	38.0
58-59	35.79649636600654	38.0	36.0	38.0	31.0	38.0
60-61	35.80448758013536	38.0	36.0	38.0	31.5	38.0
62-63	35.78609439454276	38.0	36.0	38.0	31.0	38.0
64-65	35.80220182503259	38.0	36.0	38.0	31.5	38.0
66-67	35.73739709637672	38.0	36.0	38.0	31.0	38.0
68-69	35.44766870836979	38.0	36.0	38.0	31.0	38.0
70-71	34.60113573456669	38.0	35.0	38.0	25.0	38.0
72-73	33.463329702316116	38.0	34.0	38.0	20.0	38.0
74-75	33.34180967517277	38.0	34.0	38.0	19.0	38.0
76	32.47809782317541	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	8.0
23	16.0
24	35.0
25	85.0
26	150.0
27	255.0
28	471.0
29	736.0
30	1177.0
31	1668.0
32	2618.0
33	3680.0
34	5762.0
35	8441.0
36	10828.0
37	20066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.850675904926874	18.000321434311328	11.741281094305256	32.407721566456544
2	20.10214468115502	17.944963302916122	38.368542295398136	23.584349720530724
3	18.83069340523938	21.280737156020642	34.289898033893465	25.598671404846513
4	14.602046465115448	22.716477079947854	32.945231164842234	29.736245290094466
5	14.95026696905302	33.48452650940195	36.57029589814103	14.994910623403989
6	30.268397649958033	38.1649672315577	15.689565885105091	15.877069233379167
7	27.380846086537257	27.8379971070912	18.246754406328687	26.534402400042858
8	23.736138145324023	38.582831836282786	16.821728959445704	20.859301058947484
9	22.441472169145875	16.609225164735083	17.109234093465954	43.84006857265309
10-11	25.025446882980052	25.625457597457096	26.567438704262575	22.781656815300273
12-13	24.402221468240505	18.700333934534548	27.062983267558348	29.8344613296666
14-15	23.91114127037983	19.42177538884623	19.789639100698228	36.87744424007571
16-17	28.62372542366828	26.44690083751496	19.668208360863588	25.261165377953176
18-19	28.333541670386968	23.01469669101234	20.42268611939499	28.229075519205697
20-21	23.188806942981124	22.203967927998715	20.25214735977428	34.35507776924588
22-23	23.995964213646673	26.74690619475348	20.487865854747405	28.769263736852444
24-25	28.478187110484114	27.071911998428543	20.430721977178166	24.01917891390918
26-27	27.73799532134502	28.301398239254272	21.452168788728372	22.508437650672334
28-29	22.900408935873855	28.10228754084894	22.037893533813104	26.959409989464096
30-31	22.30129109448383	22.73612028786228	26.992446293683813	27.97014232397007
32-33	22.50129466597618	26.28975517419954	22.332541652529507	28.87640850729477
34-35	23.520955731352345	22.1066447615136	26.38350684833658	27.988892658797475
36-37	27.71567349416954	25.463847568706584	22.895944570438758	23.92453436668512
38-39	23.07362631475562	22.81469311951999	26.967445847247273	27.14423471847712
40-41	22.689690887337274	20.940552509866247	28.016571724495083	28.353184878301395
42-43	27.862104680440723	20.728941588242648	27.677279951427703	23.731673779888926
44-45	23.020053929534456	25.420989660529653	28.255861711816284	23.303094698119608
46-47	27.26923695065983	20.66465472597725	24.085251522348614	27.980856801014305
48-49	26.792442722191467	19.83071126270112	23.954892051643778	29.421953963463633
50-51	22.336113144877586	19.837854247397274	29.109448383006843	28.7165842247183
52-53	21.12091287344417	19.84678297826747	34.465794032036285	24.566510116252076
54-55	20.870015535991715	20.07535848854444	30.53268808371578	28.521937891748067
56-57	25.681708601939317	20.427150484830086	25.489740888230145	28.401400025000445
58-59	21.499491062340397	21.533420239647135	32.58540331077341	24.381685387239056
60-61	25.342416828871944	27.34780978231754	24.262040393578456	23.047732995232057
62-63	20.25482597903534	34.24614725262951	21.76913873462026	23.72988803371489
64-65	19.897676744227574	35.455990285540814	21.075376346006177	23.570956624225435
66-67	19.93607028696941	35.2024143288273	20.863765424382578	23.99774995982071
68-69	19.605707244772226	35.07919784281862	20.822693262379683	24.492401650029468
70-71	20.228039786424755	33.60060001071447	21.017339595349917	25.154020607510848
72-73	20.326255825996892	30.83537206021536	21.6834229182664	27.15494919552135
74-75	20.384292576653156	29.402310755549205	22.317362810050177	27.89603385774746
76	20.370006607260844	29.573742388256935	22.434329184449723	27.6219218200325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.5
16	2.5
17	0.0
18	4.5
19	15.5
20	19.5
21	17.0
22	32.0
23	58.5
24	92.0
25	114.0
26	132.5
27	192.5
28	267.5
29	301.0
30	342.0
31	430.5
32	588.5
33	699.0
34	775.0
35	899.0
36	1074.5
37	1202.0
38	1323.5
39	1731.5
40	2871.5
41	4114.5
42	4504.0
43	4331.5
44	3739.5
45	3046.5
46	2773.0
47	2592.0
48	2219.5
49	1995.5
50	1963.0
51	1810.0
52	1517.0
53	1321.0
54	1265.0
55	1220.0
56	1178.0
57	1155.0
58	1129.0
59	1145.5
60	1186.0
61	1200.0
62	1190.0
63	1218.5
64	1269.5
65	1259.5
66	1179.5
67	1132.0
68	1071.5
69	971.0
70	890.0
71	849.0
72	776.0
73	652.5
74	541.5
75	481.0
76	421.0
77	333.0
78	264.0
79	223.0
80	175.0
81	122.0
82	98.0
83	79.0
84	61.5
85	32.5
86	17.5
87	14.0
88	9.5
89	5.0
90	3.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	55999.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.09032304148288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.61696895173687	41.60252861658244
2	17.74362127267138	20.614653833104164
3	5.923762680602521	10.32339863211843
4	2.216415616354135	5.150091965927963
5	0.9929296034429757	2.8839800710726977
6	0.5010759299108515	1.746459758209968
7	0.3135567168767292	1.275022768263719
8	0.17214878573624345	0.8000142859693924
9	0.12603750384260684	0.6589403382203254
>10	0.3412234860129112	2.7554063465419025
>50	0.018444512757454658	0.8268004785799747
>100	0.027666769136181987	3.757209950177682
>500	0.003074085459575776	1.0500187503348273
>1k	0.003074085459575776	6.555474204896516
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	3671	6.555474204896516	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	588	1.0500187503348273	RNA PCR Primer, Index 42 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	488	0.8714441329309451	No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	376	0.6714405614385971	RNA PCR Primer, Index 32 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	310	0.5535813139520349	No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	244	0.43572206646547257	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	155	0.27679065697601746	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	143	0.25536170288755156	No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	140	0.2500044643654351	RNA PCR Primer, Index 32 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	133	0.23750424114716337	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	115	0.20536081001446455	No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT	98	0.17500312505580456	RNA PCR Primer, Index 42 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	97	0.17321737888176575	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT	78	0.13928820157502814	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT	73	0.13035947070483403	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT	61	0.10893051661636814	No Hit
TCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	56	0.10000178574617405	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0017857461740388221	0.0
10	0.0	0.0	0.0	0.005357238522116467	0.0
11	0.0	0.0	0.0	0.005357238522116467	0.0
12	0.0	0.0	0.0	0.005357238522116467	0.0
13	0.0	0.0	0.0	0.005357238522116467	0.0
14	0.0	0.0	0.0	0.005357238522116467	0.0
15	0.0	0.0	0.0	0.005357238522116467	0.0
16	0.0	0.0	0.0	0.007142984696155289	0.0
17	0.0	0.0	0.0	0.007142984696155289	0.0
18	0.0	0.0	0.0	0.010714477044232933	0.0
19	0.0	0.0	0.0	0.014285969392310577	0.0
20	0.0	0.0	0.0	0.016071715566349398	0.0
21	0.0	0.0	0.0	0.01785746174038822	0.0
22	0.0	0.0	0.0	0.021428954088465867	0.0
23	0.0	0.0	0.0	0.02500044643654351	0.0
24	0.0	0.0	0.0	0.028571938784621154	0.0
25	0.0	0.0	0.0	0.030357684958659977	0.0
26	0.0	0.0	0.0	0.03392917730673762	0.0
27	0.0	0.0	0.0	0.05178663904712584	0.0
28	0.0	0.0	0.0	0.06964410078751407	0.0
29	0.0	0.0	0.0	0.08571581635386347	0.0
30	0.0	0.0	0.0	0.10714477044232933	0.0
31	0.0	0.0	0.0	0.12500223218271755	0.0
32	0.0	0.0	0.0	0.1535741709673387	0.0
33	0.0	0.0	0.0	0.20357506384042573	0.0
34	0.0	0.0	0.0	0.27321916462793977	0.0
35	0.0	0.0	0.0	0.3642922195039197	0.0
36	0.0	0.0	0.0	0.5732245218664619	0.0
37	0.0	0.0	0.0	0.883944356149217	0.0
38	0.0	0.0	0.0	1.164306505473312	0.0
39	0.0	0.0	0.0	1.5089555170628046	0.0
40	0.0	0.0	0.0	1.9053911676994233	0.0
41	0.0	0.0	0.0	2.3928998732120217	0.0
42	0.0	0.0	0.0	2.9446954409900177	0.0
43	0.0	0.0	0.0	3.5786353327737994	0.0
44	0.0	0.0	0.0	4.137573885247951	0.0
45	0.0	0.0	0.0	4.737584599724995	0.0
46	0.0	0.0	0.0	5.389381953249165	0.0
47	0.0	0.0	0.0	6.089394453472384	0.0
48	0.0	0.0	0.0	6.717977106734049	0.0
49	0.0	0.0	0.0	7.346559759995714	0.0
50	0.0	0.0	0.0	7.92871301273237	0.0
51	0.0	0.0	0.0	8.534080965731532	0.0
52	0.0	0.0	0.0	9.15730638047108	0.0
53	0.0	0.0	0.0	9.800175003125055	0.0
54	0.0	0.0	0.0	10.389471240557867	0.0
55	0.0	0.0	0.0	10.984124716512795	0.0
56	0.0	0.0	0.0	11.559134984553296	0.0
57	0.0	0.0	0.0	12.184146145466883	0.0
58	0.0	0.0	0.0	12.714512759156413	0.0
59	0.0	0.0	0.0	13.28059429632672	0.0
60	0.0	0.0	0.0	13.837747102626833	0.0
61	0.0	0.0	0.0	14.377042447186557	0.0
62	0.0	0.0	0.0	14.93240950731263	0.0
63	0.0	0.0	0.0	15.49491955213486	0.0
64	0.0	0.0	0.0	16.02171467347631	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTATTC	15	0.0022138285	70.0	16
TTCCATC	15	0.0022138285	70.0	3
TTCGGCT	15	0.0022138285	70.0	20
TCTTTAT	45	3.7471182E-10	62.222225	1
TTAATAC	25	2.3713624E-4	56.000004	2
TTTACAC	25	2.3713624E-4	56.000004	2
CTTTATA	50	9.531504E-10	56.000004	2
TGTGGAA	20	0.0069176937	52.5	49
ATTCGGC	20	0.0069176937	52.5	19
TCGGCTA	20	0.0069176937	52.5	21
GAGACGT	20	0.0069176937	52.5	24
TATTCGG	20	0.0069176937	52.5	18
CGGCTAT	20	0.0069176937	52.5	22
CCGAAAA	20	0.0069176937	52.5	27
ACCCGAT	20	0.0069176937	52.5	32
TTCGAAC	20	0.0069176937	52.5	57
ACTCGAC	45	1.6570011E-6	46.666668	32
CGAACTC	30	5.8313156E-4	46.666664	35
AACTCGT	30	5.8313156E-4	46.666664	37
AATACAC	40	4.5387285E-5	43.75	4
>>END_MODULE