FastQCFastQC Report
Wed 25 May 2016
SRR1779761_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779761_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22394
Sequences flagged as poor quality0
Sequence length76
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGTG207.7600256E-570.0000144
CTTATAT207.7600256E-570.000011
CAAGCCC207.7600256E-570.0000129
GCCTCTG150.002196392370.014
AGTGTAG150.002196392370.035
GATCTCT150.002196392370.041
CTTAAAC150.002196392370.062
TACCCTT150.002196392370.039
CTTTGTG150.002196392370.032
CGTCTCA150.002196392370.056
GTTGTCC150.002196392370.054
TAGGTTG150.002196392370.045
TGGTTGC252.3404977E-455.99999649
CCCTCTG252.3404977E-455.9999968
CCAAGCC252.3404977E-455.99999628
TCTTACG252.3404977E-455.99999611
GTAGATC407.15665E-752.50000438
GTGTAGG200.00686362452.50000436
TCTGGGG200.00686362452.50000444
TCGGCCC200.00686362452.50000429