FastQCFastQC Report
Wed 25 May 2016
SRR1779761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22394
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT433719.366794677145663No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT7333.2731981780834154RNA PCR Primer, Index 15 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG6122.73287487719925No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC4051.8085201393230328No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3711.656693757256408No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2381.062784674466375No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1690.754666428507636No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1410.6296329373939448No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1310.5849781191390551No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT1290.5760471554880772No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1220.5447887827096544No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1160.5179958917567206No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT860.38403143699205144No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT720.3215146914352059No Hit
TCTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT530.23667053675091543No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT490.21880860944895955No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC470.2098776457979816No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCG370.1652228275430919No Hit
CTTATACACATCTCCGAGCCCACGAGACATTAGTTAATCTCGTATGCCGT330.14736090024113602No Hit
CTCTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG330.14736090024113602No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT310.1384299365901581No Hit
CTTATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC310.1384299365901581No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGTCTT270.12056800928820219No Hit
CTTATTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG270.12056800928820219No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG260.11610252746271323No Hit
CTTATAAACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT240.10717156381173527No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGCTAATCTCGTATGCCGT240.10717156381173527No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGCT207.7600256E-570.0000129
TAAGCTA207.7600256E-570.0000130
AGCTAAT207.7600256E-570.0000132
AAGCTAA207.7600256E-570.0000131
CATAAGC207.7600256E-570.0000128
CTTTACA150.002196392370.01
TTATTAC150.002196392370.02
ACACTAA150.002196392370.070
ATTACAC150.002196392370.04
GCTAATC252.7508559E-670.033
CTTATTA150.002196392370.01
CTAATCT252.7508559E-670.034
TATTACA150.002196392370.03
TCTTTAT500.070.01
TTTACAC150.002196392370.02
AACACTA150.002196392370.069
CTATACA401.00499165E-861.2500041
AAACACT252.3404977E-455.99999668
GCAGCGT200.00686362452.5000041
TCCGGCG200.00686362452.50000416
Produced by FastQC (version 0.11.5)