##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779760_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11050
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.486968325791857	34.0	27.0	34.0	12.0	34.0
2	27.815384615384616	34.0	23.0	34.0	12.0	34.0
3	27.15683257918552	32.0	23.0	34.0	11.0	34.0
4	29.44841628959276	34.0	27.0	34.0	12.0	34.0
5	28.96552036199095	34.0	23.0	34.0	11.0	34.0
6	31.449411764705882	37.0	27.0	38.0	11.0	38.0
7	32.33049773755656	37.0	28.0	38.0	12.0	38.0
8	31.814570135746607	37.0	27.0	38.0	11.0	38.0
9	33.1764705882353	37.0	34.0	38.0	12.0	38.0
10-11	32.76814479638009	38.0	33.5	38.0	11.0	38.0
12-13	31.771809954751134	37.0	29.5	38.0	11.0	38.0
14-15	31.7931221719457	37.0	29.0	38.0	11.5	38.0
16-17	31.542262443438915	36.5	28.5	38.0	11.0	38.0
18-19	31.956199095022626	37.0	29.0	38.0	11.5	38.0
20-21	31.083031674208144	36.0	27.0	38.0	11.0	38.0
22-23	31.082398190045247	36.0	28.0	38.0	11.0	38.0
24-25	32.292307692307695	37.0	30.0	38.0	11.0	38.0
26-27	30.805067873303166	36.5	25.0	38.0	11.0	38.0
28-29	32.93660633484163	37.5	33.5	38.0	11.0	38.0
30-31	33.38054298642534	38.0	34.0	38.0	11.0	38.0
32-33	33.43334841628959	38.0	34.0	38.0	11.0	38.0
34-35	33.55289592760181	38.0	34.0	38.0	11.0	38.0
36-37	31.561764705882354	37.0	28.0	38.0	11.0	38.0
38-39	29.65701357466063	35.0	23.0	38.0	11.0	38.0
40-41	30.88904977375566	36.0	26.5	38.0	11.0	38.0
42-43	32.03981900452489	37.0	30.0	38.0	11.0	38.0
44-45	33.59633484162896	38.0	34.5	38.0	16.0	38.0
46-47	32.16371040723982	37.5	29.5	38.0	11.0	38.0
48-49	31.54081447963801	36.5	28.5	38.0	16.0	38.0
50-51	29.433529411764706	35.0	22.5	38.0	11.0	38.0
52-53	32.787013574660634	37.0	32.5	38.0	11.0	38.0
54-55	31.919592760180997	37.0	29.5	38.0	11.0	38.0
56-57	32.24384615384616	37.5	30.0	38.0	11.0	38.0
58-59	33.321312217194574	38.0	34.0	38.0	11.0	38.0
60-61	33.786923076923074	38.0	36.0	38.0	20.5	38.0
62-63	33.6410407239819	38.0	35.0	38.0	11.0	38.0
64-65	33.35977375565611	38.0	35.0	38.0	11.0	38.0
66-67	31.63099547511312	37.0	27.0	38.0	11.0	38.0
68-69	31.552805429864254	37.0	27.0	38.0	11.0	38.0
70-71	30.988733031674208	36.5	25.5	38.0	11.0	38.0
72-73	29.856968325791854	35.0	23.5	38.0	11.0	38.0
74-75	31.940135746606334	36.5	30.0	38.0	11.0	38.0
76	32.533574660633484	37.0	32.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	20.0
14	59.0
15	103.0
16	177.0
17	234.0
18	259.0
19	287.0
20	249.0
21	227.0
22	168.0
23	120.0
24	116.0
25	117.0
26	136.0
27	134.0
28	163.0
29	205.0
30	291.0
31	375.0
32	501.0
33	855.0
34	1167.0
35	1696.0
36	2197.0
37	1191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.18552036199095	20.38009049773756	12.60633484162896	35.828054298642535
2	17.44796380090498	16.895927601809955	41.755656108597286	23.900452488687783
3	17.32126696832579	17.429864253393664	37.0316742081448	28.217194570135746
4	10.895927601809955	19.20361990950226	33.918552036199095	35.98190045248869
5	12.39819004524887	29.6289592760181	40.77828054298642	17.194570135746606
6	23.945701357466064	34.71493212669684	16.914027149321267	24.425339366515836
7	23.51131221719457	29.30316742081448	20.38914027149321	26.796380090497742
8	20.488687782805428	33.7737556561086	17.6289592760181	28.108597285067873
9	20.46153846153846	16.16289592760181	19.257918552036198	44.11764705882353
10-11	21.380090497737555	24.923076923076923	28.167420814479637	25.529411764705884
12-13	21.429864253393667	20.823529411764703	29.81447963800905	27.932126696832576
14-15	21.149321266968325	23.09049773755656	24.144796380090497	31.615384615384617
16-17	22.751131221719454	24.542986425339368	25.669683257918553	27.036199095022624
18-19	18.914027149321267	25.33031674208145	28.34841628959276	27.407239819004523
20-21	22.34841628959276	24.79638009049774	26.27601809954751	26.579185520361992
22-23	22.601809954751133	23.67420814479638	26.823529411764707	26.900452488687783
24-25	19.74660633484163	26.529411764705884	24.850678733031675	28.873303167420815
26-27	21.031674208144796	25.122171945701353	27.733031674208146	26.1131221719457
28-29	20.104072398190045	25.86425339366516	27.41628959276018	26.615384615384613
30-31	20.004524886877828	26.416289592760183	27.239819004524886	26.339366515837103
32-33	20.36651583710407	25.769230769230766	26.855203619909503	27.009049773755656
34-35	19.84162895927602	25.846153846153847	27.339366515837106	26.972850678733028
36-37	21.529411764705884	23.74660633484163	30.438914027149323	24.285067873303166
38-39	21.710407239819006	22.43891402714932	33.4841628959276	22.366515837104075
40-41	19.914027149321267	25.15837104072398	26.823529411764707	28.104072398190045
42-43	18.56561085972851	26.18099547511312	27.624434389140273	27.6289592760181
44-45	17.877828054298643	25.588235294117645	28.61990950226244	27.914027149321267
46-47	21.69230769230769	24.02714932126697	28.53393665158371	25.746606334841626
48-49	21.8868778280543	24.108597285067873	28.728506787330314	25.27601809954751
50-51	24.330316742081447	22.199095022624434	29.434389140271495	24.036199095022624
52-53	18.466063348416288	24.95022624434389	28.886877828054295	27.69683257918552
54-55	21.04977375565611	24.63348416289593	27.542986425339365	26.773755656108598
56-57	21.040723981900452	25.02714932126697	27.34841628959276	26.58371040723982
58-59	19.321266968325794	25.687782805429865	28.34841628959276	26.642533936651585
60-61	18.58823529411765	25.325791855203622	27.877828054298643	28.20814479638009
62-63	18.402714932126695	25.674208144796378	28.56561085972851	27.35746606334842
64-65	19.39819004524887	26.647058823529413	27.633484162895925	26.32126696832579
66-67	17.90950226244344	26.294117647058822	24.60633484162896	31.19004524886878
68-69	18.185932832443196	26.65882139947497	24.468181406716756	30.687064361365074
70-71	18.171945701357465	27.203619909502265	24.36651583710407	30.2579185520362
72-73	17.475113122171944	25.782805429864254	24.226244343891405	32.515837104072396
74-75	19.33031674208145	26.475113122171944	25.520361990950224	28.67420814479638
76	20.289592760180998	24.97737556561086	26.407239819004523	28.32579185520362
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	2.0
19	2.5
20	5.0
21	8.0
22	11.5
23	16.5
24	18.0
25	18.0
26	27.0
27	45.5
28	78.5
29	102.0
30	99.5
31	130.5
32	194.5
33	225.0
34	226.5
35	264.0
36	328.0
37	356.0
38	399.5
39	463.0
40	514.5
41	586.0
42	626.0
43	628.0
44	635.5
45	634.0
46	627.0
47	623.0
48	610.0
49	575.0
50	549.0
51	523.5
52	457.0
53	370.5
54	325.0
55	306.0
56	277.0
57	243.0
58	219.0
59	213.5
60	185.5
61	145.5
62	128.0
63	134.5
64	131.0
65	126.5
66	131.0
67	130.0
68	114.0
69	84.5
70	81.5
71	92.0
72	96.0
73	86.0
74	64.5
75	57.0
76	51.5
77	32.5
78	19.0
79	19.0
80	18.0
81	15.5
82	10.0
83	6.0
84	6.5
85	3.5
86	1.5
87	3.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.027149321266968326
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	11050.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.32579185520362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.6010124252186	96.9502262443439
2	1.1780947998159228	2.3167420814479636
3	0.14726184997699035	0.4343891402714932
4	0.06442705936493327	0.25339366515837103
5	0.009203865623561897	0.04524886877828054
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.00904977375565611
10	0.0	0.0	0.0	0.0	0.00904977375565611
11	0.0	0.0	0.0	0.0	0.00904977375565611
12	0.0	0.0	0.0	0.0	0.00904977375565611
13	0.0	0.0	0.0	0.0	0.00904977375565611
14	0.0	0.0	0.0	0.03619909502262444	0.00904977375565611
15	0.0	0.0	0.0	0.03619909502262444	0.00904977375565611
16	0.0	0.0	0.0	0.03619909502262444	0.00904977375565611
17	0.0	0.0	0.0	0.03619909502262444	0.00904977375565611
18	0.0	0.0	0.0	0.03619909502262444	0.00904977375565611
19	0.0	0.0	0.0	0.03619909502262444	0.00904977375565611
20	0.0	0.0	0.0	0.04524886877828054	0.00904977375565611
21	0.0	0.0	0.0	0.08144796380090498	0.00904977375565611
22	0.0	0.0	0.0	0.11764705882352941	0.00904977375565611
23	0.0	0.0	0.0	0.12669683257918551	0.00904977375565611
24	0.0	0.0	0.0	0.14479638009049775	0.00904977375565611
25	0.0	0.0	0.0	0.16289592760180996	0.00904977375565611
26	0.0	0.0	0.0	0.16289592760180996	0.00904977375565611
27	0.0	0.0	0.0	0.16289592760180996	0.00904977375565611
28	0.0	0.0	0.0	0.16289592760180996	0.00904977375565611
29	0.0	0.0	0.0	0.17194570135746606	0.00904977375565611
30	0.0	0.0	0.0	0.18099547511312217	0.00904977375565611
31	0.0	0.0	0.0	0.18099547511312217	0.00904977375565611
32	0.0	0.0	0.0	0.18099547511312217	0.00904977375565611
33	0.0	0.0	0.0	0.18099547511312217	0.00904977375565611
34	0.0	0.0	0.0	0.2081447963800905	0.00904977375565611
35	0.0	0.0	0.0	0.24434389140271492	0.00904977375565611
36	0.0	0.0	0.0	0.2986425339366516	0.00904977375565611
37	0.0	0.0	0.0	0.3891402714932127	0.00904977375565611
38	0.0	0.0	0.0	0.45248868778280543	0.00904977375565611
39	0.0	0.0	0.0	0.5701357466063348	0.00904977375565611
40	0.0	0.0	0.0	0.6877828054298643	0.00904977375565611
41	0.0	0.0	0.0	0.8416289592760181	0.00904977375565611
42	0.0	0.0	0.0	0.9683257918552036	0.00904977375565611
43	0.0	0.0	0.0	1.0407239819004526	0.00904977375565611
44	0.0	0.0	0.0	1.1312217194570136	0.00904977375565611
45	0.0	0.0	0.0	1.1945701357466063	0.00904977375565611
46	0.0	0.0	0.0	1.339366515837104	0.00904977375565611
47	0.0	0.0	0.0	1.583710407239819	0.00904977375565611
48	0.0	0.0	0.0	1.7194570135746607	0.00904977375565611
49	0.0	0.0	0.0	1.8552036199095023	0.00904977375565611
50	0.0	0.0	0.0	2.0	0.00904977375565611
51	0.0	0.0	0.0	2.235294117647059	0.00904977375565611
52	0.0	0.0	0.0	2.434389140271493	0.00904977375565611
53	0.0	0.0	0.0	2.669683257918552	0.00904977375565611
54	0.0	0.0	0.0	2.9049773755656108	0.00904977375565611
55	0.0	0.0	0.0	3.158371040723982	0.00904977375565611
56	0.0	0.0	0.0	3.2941176470588234	0.00904977375565611
57	0.0	0.0	0.0	3.411764705882353	0.00904977375565611
58	0.0	0.0	0.0	3.5565610859728505	0.00904977375565611
59	0.0	0.0	0.0	3.683257918552036	0.00904977375565611
60	0.0	0.0	0.0	3.8099547511312215	0.00904977375565611
61	0.0	0.0	0.0	3.936651583710407	0.00904977375565611
62	0.0	0.0	0.0	4.0633484162895925	0.00904977375565611
63	0.0	0.0	0.0	4.262443438914027	0.00904977375565611
64	0.0	0.0	0.0	4.416289592760181	0.00904977375565611
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATGT	20	7.603865E-5	69.96833	31
TATGTGT	15	0.002170782	69.96832	33
TTCTATG	15	0.002170782	69.96832	30
CTATGTG	15	0.002170782	69.96832	32
TTTCTAT	20	0.0067842584	52.476246	29
>>END_MODULE