FastQCFastQC Report
Wed 25 May 2016
SRR1779760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11050
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT4013.6289592760180995Illumina PCR Primer Index 9 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1721.5565610859728507No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1551.402714932126697No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1351.2217194570135748No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG620.5610859728506787No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC580.5248868778280543No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT540.48868778280542985No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC420.3800904977375566No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC420.3800904977375566No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGTCTT210.1900452488687783No Hit
CTTATAAACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT190.17194570135746606No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG180.16289592760180996No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGTCTT150.13574660633484162Illumina Single End Adapter 1 (95% over 21bp)
TCTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT150.13574660633484162No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG150.13574660633484162No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGTC120.1085972850678733No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAG120.1085972850678733No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGTC120.1085972850678733No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG120.1085972850678733No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC309.335963E-870.02
CTTTACA352.7135138E-760.01
TTACACA352.7135138E-760.03
CTATACA305.628917E-446.6666641
TGCCGTC1753.983587E-922.044
CCGTCTT1753.983587E-922.046
GCCGTCT1753.983587E-922.045
CGTCTTC1753.983587E-922.047
TGCTTGA1805.515176E-921.38888754
ATGCCGT1805.515176E-921.38888743
TCTTCTG1805.515176E-921.38888749
GTATGCC1857.568815E-920.8108141
ACTTGCT1857.568815E-920.8108126
TATGCCG1857.568815E-920.8108142
TCTGCTT1857.568815E-920.8108152
GTCTTCT1857.568815E-920.8108148
CTTGCTA1857.568815E-920.8108127
GCTTGAA1857.568815E-920.8108155
TGCTAAG1857.568815E-920.8108129
CTGCTTG1857.568815E-920.8108153
Produced by FastQC (version 0.11.5)