FastQCFastQC Report
Wed 25 May 2016
SRR1779759_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779759_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24832
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATC207.776901E-570.038
AGATCTC308.141871E-658.33333240
TATATCT308.141871E-658.33333239
TTCCGCT252.3455557E-455.99999613
GGTCGCC200.00687250352.550
ATGGAGA200.00687250352.545
AAGCAAT200.00687250352.543
GCTGTGT200.00687250352.533
TATACCC200.00687250352.53
CTGATAC200.00687250352.51
GGCCTCA200.00687250352.51
ATACCCA200.00687250352.54
CTGGAGG200.00687250352.545
GTAGATC352.0284258E-550.038
TCTCGGT352.0284258E-550.043
TAGATCT352.0284258E-550.039
GTGTAGA305.7682337E-446.66666436
TACCCCT350.001232165740.05
ATCTCGG458.9441295E-538.8888942
TGTAGAT458.9441295E-538.8888937
Produced by FastQC (version 0.11.5)