##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779759_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24832
Sequences flagged as poor quality	0
Sequence length	76
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.65004832474227	33.0	23.0	34.0	12.0	34.0
2	27.090649162371133	32.0	21.0	34.0	12.0	34.0
3	26.570151417525775	32.0	21.0	34.0	11.0	34.0
4	28.595884342783506	33.0	23.0	34.0	12.0	34.0
5	28.010188466494846	34.0	23.0	34.0	11.0	34.0
6	30.3278431056701	36.0	26.0	38.0	11.0	38.0
7	31.43234536082474	37.0	27.0	38.0	12.0	38.0
8	30.711541559278352	37.0	26.0	38.0	11.0	38.0
9	32.18826514175258	37.0	28.0	38.0	12.0	38.0
10-11	31.762383215206185	37.0	27.5	38.0	11.0	38.0
12-13	31.014316204896907	36.0	27.0	38.0	11.0	38.0
14-15	30.833480992268044	36.5	26.5	38.0	11.0	38.0
16-17	30.567090850515463	35.5	25.0	38.0	11.0	38.0
18-19	30.98218025128866	36.5	26.5	38.0	11.5	38.0
20-21	30.02879349226804	35.5	24.0	38.0	11.0	38.0
22-23	30.121758215206185	35.5	24.5	38.0	11.0	38.0
24-25	31.17843911082474	36.5	27.0	38.0	11.0	38.0
26-27	29.562922841494846	35.0	22.5	38.0	11.0	38.0
28-29	31.69219152706186	37.0	29.0	38.0	11.0	38.0
30-31	32.202379993556704	37.5	31.0	38.0	11.0	38.0
32-33	32.347777061855666	38.0	31.0	38.0	11.0	38.0
34-35	32.34125322164948	38.0	31.0	38.0	11.0	38.0
36-37	30.286847615979383	36.0	24.5	38.0	11.0	38.0
38-39	28.715125644329895	34.0	22.0	37.5	11.0	38.0
40-41	29.785478414948454	35.0	24.0	38.0	11.0	38.0
42-43	30.80742590206186	36.5	25.0	38.0	11.0	38.0
44-45	32.48073050902062	37.5	31.5	38.0	11.0	38.0
46-47	30.99484536082474	36.5	27.5	38.0	11.0	38.0
48-49	30.19460776417526	35.5	24.5	38.0	11.0	38.0
50-51	28.177170586340203	33.5	21.0	37.5	11.0	38.0
52-53	31.409089078608247	36.5	28.0	38.0	11.0	38.0
54-55	30.527625644329895	36.0	25.0	38.0	11.0	38.0
56-57	31.023699259020617	36.5	27.0	38.0	11.0	38.0
58-59	32.01455782860825	37.0	31.0	38.0	11.0	38.0
60-61	32.52931701030928	38.0	32.5	38.0	11.0	38.0
62-63	32.436976481958766	38.0	32.0	38.0	11.0	38.0
64-65	32.159431378865975	38.0	30.5	38.0	11.0	38.0
66-67	30.448574420103093	36.5	24.5	38.0	11.0	38.0
68-69	30.277746456185568	36.0	24.5	38.0	11.0	38.0
70-71	29.7085212628866	35.0	23.5	38.0	11.0	38.0
72-73	28.629208279639176	34.0	21.5	37.5	11.0	38.0
74-75	30.391672036082475	35.5	25.0	38.0	11.0	38.0
76	30.89489368556701	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	8.0
13	87.0
14	169.0
15	329.0
16	484.0
17	650.0
18	883.0
19	847.0
20	729.0
21	574.0
22	471.0
23	349.0
24	304.0
25	314.0
26	321.0
27	396.0
28	452.0
29	517.0
30	724.0
31	948.0
32	1365.0
33	1847.0
34	2641.0
35	3729.0
36	3946.0
37	1748.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.07297036082475	18.637242268041238	12.141591494845361	35.14819587628865
2	19.635953608247423	15.089400773195877	39.83569587628865	25.43894974226804
3	21.967622422680414	14.892074742268042	35.23276417525773	27.90753865979381
4	16.245167525773198	17.082796391752577	27.569265463917525	39.102770618556704
5	16.82506443298969	25.197326030927837	36.75499355670103	21.222615979381445
6	31.064755154639172	30.754671391752574	13.732280927835053	24.448292525773198
7	27.25112757731959	23.40125644329897	17.763369845360824	31.584246134020617
8	22.97036082474227	30.819104381443296	17.23582474226804	28.974710051546392
9	22.62403350515464	13.957796391752577	18.246617268041238	45.17155283505155
10-11	26.82828608247423	21.244764819587626	24.468427835051546	27.4585212628866
12-13	24.750322164948454	16.1706668814433	27.752496778350515	31.32651417525773
14-15	26.093347293814436	19.55943943298969	20.612516108247423	33.73469716494845
16-17	28.187419458762886	19.080219072164947	23.040834407216497	29.691527061855673
18-19	24.4543331185567	18.613079896907216	25.638289304123713	31.29429768041237
20-21	27.245086984536083	18.371456185567013	24.252980025773198	30.130476804123713
22-23	28.286082474226802	17.835856958762886	23.5120006443299	30.36605992268041
24-25	25.7007087628866	20.962870489690722	20.356797680412374	32.97962306701031
26-27	26.641027706185564	21.041398195876287	23.971085695876287	28.346488402061855
28-29	26.320876288659793	21.581024484536083	22.334085051546392	29.76401417525773
30-31	25.8960212628866	20.91454574742268	22.913981958762886	30.275451030927837
32-33	26.177915592783506	20.423244201030926	23.143524484536083	30.255315721649485
34-35	25.894007731958762	20.983005798969074	23.13547036082474	29.987516108247426
36-37	27.51490012886598	17.884181701030926	27.581346649484534	27.019571520618555
38-39	26.71351481958763	16.923727448453608	31.594313788659793	24.76844394329897
40-41	26.19402384020619	21.204494201030926	21.64747100515464	30.954010953608247
42-43	23.824097938144327	21.127980025773198	23.785840850515463	31.26208118556701
44-45	23.3629993556701	18.834568298969074	26.026900773195877	31.77553157216495
46-47	26.71351481958763	18.349307345360824	25.636275773195877	29.300902061855673
48-49	26.914867912371133	18.707715850515463	26.22624033505155	28.151175902061855
50-51	29.818379510309278	17.088836984536083	27.412210051546392	25.680573453608247
52-53	23.230106314432987	20.463514819587626	25.318137886597935	30.988240979381445
54-55	26.222213273195877	19.070151417525775	24.3737918814433	30.33384342783505
56-57	26.308795103092784	19.51514175257732	24.057667525773198	30.118395618556704
58-59	23.46568943298969	21.24073775773196	24.512725515463917	30.780847293814436
60-61	23.449581185567013	19.617831829896907	24.619442654639176	32.31314432989691
62-63	22.95827963917526	20.72728737113402	24.60132087628866	31.713112113402065
64-65	23.630798969072163	22.227367912371136	23.79188144329897	30.34995167525773
66-67	21.887081185567013	24.21069587628866	20.370892396907216	33.53133054123711
68-69	22.343274865577865	24.43160078136013	20.6113941639648	32.613730189097204
70-71	22.398518041237114	25.165109536082475	19.772873711340207	32.6634987113402
72-73	22.247503221649485	25.15906894329897	18.83859536082474	33.7548324742268
74-75	23.997261597938145	22.374355670103093	21.856878221649485	31.771504510309278
76	26.014819587628867	20.8239368556701	21.975676546391753	31.185567010309278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	0.5
20	1.0
21	2.0
22	4.0
23	6.5
24	15.0
25	23.0
26	23.0
27	25.5
28	41.0
29	54.0
30	70.0
31	99.0
32	126.5
33	141.0
34	161.5
35	213.5
36	279.5
37	314.0
38	379.0
39	474.5
40	556.5
41	656.0
42	704.0
43	709.5
44	775.0
45	854.0
46	873.0
47	865.0
48	874.0
49	867.0
50	843.0
51	851.5
52	868.0
53	888.0
54	900.0
55	898.5
56	952.5
57	992.0
58	976.0
59	1026.0
60	1071.5
61	1074.5
62	1082.0
63	1089.5
64	1088.0
65	1048.0
66	948.5
67	880.0
68	838.0
69	727.0
70	595.5
71	533.0
72	478.5
73	383.0
74	291.5
75	241.0
76	203.5
77	155.0
78	108.0
79	72.0
80	67.5
81	51.5
82	30.5
83	21.0
84	13.5
85	4.5
86	4.0
87	5.0
88	3.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.01409471649484536
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	24832.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.14223582474226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.17949551159674	98.3287693298969
2	0.7758235509159592	1.5383376288659796
3	0.04468093748730655	0.1328930412371134
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004027061855670103	0.0
2	0.0	0.0	0.0	0.004027061855670103	0.0
3	0.0	0.0	0.0	0.004027061855670103	0.0
4	0.0	0.0	0.0	0.004027061855670103	0.0
5	0.0	0.0	0.0	0.004027061855670103	0.0
6	0.0	0.0	0.0	0.004027061855670103	0.0
7	0.0	0.0	0.0	0.004027061855670103	0.0
8	0.0	0.0	0.0	0.004027061855670103	0.0
9	0.0	0.0	0.0	0.004027061855670103	0.0
10	0.0	0.0	0.0	0.004027061855670103	0.0
11	0.0	0.0	0.0	0.004027061855670103	0.0
12	0.0	0.0	0.0	0.004027061855670103	0.0
13	0.0	0.0	0.0	0.004027061855670103	0.0
14	0.0	0.0	0.0	0.004027061855670103	0.0
15	0.0	0.0	0.0	0.004027061855670103	0.0
16	0.0	0.0	0.0	0.004027061855670103	0.0
17	0.0	0.0	0.0	0.004027061855670103	0.0
18	0.0	0.0	0.0	0.004027061855670103	0.0
19	0.0	0.0	0.0	0.008054123711340205	0.0
20	0.0	0.0	0.0	0.01208118556701031	0.0
21	0.0	0.0	0.0	0.01610824742268041	0.0
22	0.0	0.0	0.0	0.03221649484536082	0.0
23	0.0	0.0	0.0	0.03221649484536082	0.0
24	0.0	0.0	0.0	0.040270618556701034	0.0
25	0.0	0.0	0.0	0.040270618556701034	0.0
26	0.0	0.0	0.0	0.040270618556701034	0.0
27	0.0	0.0	0.0	0.04832474226804124	0.0
28	0.0	0.0	0.0	0.06040592783505155	0.0
29	0.0	0.0	0.0	0.07248711340206186	0.0
30	0.0	0.0	0.0	0.08456829896907217	0.0
31	0.0	0.0	0.0	0.09262242268041238	0.0
32	0.0	0.0	0.0	0.10470360824742268	0.0
33	0.0	0.0	0.0	0.11678479381443299	0.0
34	0.0	0.0	0.0	0.14497422680412372	0.0
35	0.0	0.0	0.0	0.21746134020618557	0.0
36	0.0	0.0	0.0	0.2416237113402062	0.0
37	0.0	0.0	0.0	0.4067332474226804	0.0
38	0.0	0.0	0.0	0.5396262886597938	0.0
39	0.0	0.0	0.0	0.6604381443298969	0.0
40	0.0	0.0	0.0	0.7691688144329897	0.0
41	0.0	0.0	0.0	1.014819587628866	0.0
42	0.0	0.0	0.0	1.1436855670103092	0.0
43	0.0	0.0	0.0	1.365173969072165	0.0
44	0.0	0.0	0.0	1.643041237113402	0.0
45	0.0	0.0	0.0	1.800096649484536	0.0
46	0.0	0.0	0.0	2.0215850515463916	0.0
47	0.0	0.0	0.0	2.3155605670103094	0.0
48	0.0	0.0	0.0	2.4524806701030926	0.0
49	0.0	0.0	0.0	2.6256443298969074	0.0
50	0.0	0.0	0.0	2.935728092783505	0.0
51	0.0	0.0	0.0	3.1531894329896906	0.0
52	0.0	0.0	0.0	3.3464884020618557	0.0
53	0.0	0.0	0.0	3.636436855670103	0.0
54	0.0	0.0	0.0	3.9747100515463916	0.0
55	0.0	0.0	0.0	4.212306701030927	0.0
56	0.0	0.0	0.0	4.417686855670103	0.0
57	0.0	0.0	0.0	4.639175257731959	0.0
58	0.0	0.0	0.0	4.892880154639175	0.0
59	0.0	0.0	0.0	5.126449742268041	0.0
60	0.0	0.0	0.0	5.287532216494846	0.0
61	0.0	0.0	0.0	5.500966494845361	0.0
62	0.0	0.0	0.0	5.597615979381444	0.0
63	0.0	0.0	0.0	5.770779639175258	0.0
64	0.0	0.0	0.0	5.939916237113402	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATATC	20	7.776901E-5	70.0	38
AGATCTC	30	8.141871E-6	58.333332	40
TATATCT	30	8.141871E-6	58.333332	39
TTCCGCT	25	2.3455557E-4	55.999996	13
GGTCGCC	20	0.006872503	52.5	50
ATGGAGA	20	0.006872503	52.5	45
AAGCAAT	20	0.006872503	52.5	43
GCTGTGT	20	0.006872503	52.5	33
TATACCC	20	0.006872503	52.5	3
CTGATAC	20	0.006872503	52.5	1
GGCCTCA	20	0.006872503	52.5	1
ATACCCA	20	0.006872503	52.5	4
CTGGAGG	20	0.006872503	52.5	45
GTAGATC	35	2.0284258E-5	50.0	38
TCTCGGT	35	2.0284258E-5	50.0	43
TAGATCT	35	2.0284258E-5	50.0	39
GTGTAGA	30	5.7682337E-4	46.666664	36
TACCCCT	35	0.0012321657	40.0	5
ATCTCGG	45	8.9441295E-5	38.88889	42
TGTAGAT	45	8.9441295E-5	38.88889	37
>>END_MODULE