FastQCFastQC Report
Wed 25 May 2016
SRR1779759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779759_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24832
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT18077.276900773195877No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT5422.1826675257731956RNA PCR Primer, Index 30 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC3241.3047680412371134No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG2581.0389819587628866No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2330.938305412371134No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC2080.8376288659793815No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1060.4268685567010309No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1020.41076030927835055No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT1020.41076030927835055RNA PCR Primer, Index 30 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC870.350354381443299No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT760.30605670103092786No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG670.2698131443298969No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT570.2295425257731959No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC510.20538015463917528No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT410.16510953608247422No Hit
TCTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT350.1409471649484536No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT350.1409471649484536No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG300.1208118556701031No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCATG150.00219925570.063
TTACACA309.8354576E-870.03
ATTGCAG150.00219925570.026
GCTTGAC207.776901E-570.056
CTTGACA150.00219925570.057
TCTTTAT353.5179255E-970.01
CTTTACA352.8581053E-760.01
TTTACAC352.8581053E-760.02
AAACAAC200.00687250352.569
AACTCGT200.00687250352.537
CGGAACT200.00687250352.534
TTGACAA200.00687250352.558
CTTTATA505.878246E-848.9999962
TTTATAC551.2444434E-744.5454523
CGCTTGA350.001232165740.055
CTATACA458.9441295E-538.888891
GCCGGAA400.00237423135.032
CTTATAC5200.034.3269231
GAAAAAA5200.033.65384760
CCGTCTT5200.033.65384747
Produced by FastQC (version 0.11.5)