##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779759_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24832
Sequences flagged as poor quality	0
Sequence length	76
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.26103414948454	34.0	34.0	34.0	32.0	34.0
2	33.30424452319588	34.0	34.0	34.0	33.0	34.0
3	33.33271585051546	34.0	34.0	34.0	33.0	34.0
4	33.420666881443296	34.0	34.0	34.0	34.0	34.0
5	33.37657055412371	34.0	34.0	34.0	34.0	34.0
6	36.64888047680412	38.0	37.0	38.0	34.0	38.0
7	36.70485663659794	38.0	37.0	38.0	34.0	38.0
8	36.400209407216494	38.0	37.0	38.0	34.0	38.0
9	36.46278994845361	38.0	37.0	38.0	34.0	38.0
10-11	36.30734536082474	38.0	37.0	38.0	34.0	38.0
12-13	36.3271987757732	38.0	37.0	38.0	34.0	38.0
14-15	36.33619925902062	38.0	37.0	38.0	34.0	38.0
16-17	36.13983972293815	38.0	36.0	38.0	33.0	38.0
18-19	36.20521907216495	38.0	36.5	38.0	34.0	38.0
20-21	36.33875644329897	38.0	37.0	38.0	34.0	38.0
22-23	36.172156894329895	38.0	36.5	38.0	33.5	38.0
24-25	36.07321198453609	38.0	36.0	38.0	32.5	38.0
26-27	35.838474548969074	38.0	36.0	38.0	31.5	38.0
28-29	35.71991784793815	38.0	36.0	38.0	31.0	38.0
30-31	35.67288176546391	38.0	36.0	38.0	31.0	38.0
32-33	35.32075547680412	38.0	36.0	38.0	31.0	38.0
34-35	35.15818298969072	38.0	35.5	38.0	29.0	38.0
36-37	35.1069587628866	38.0	35.0	38.0	29.0	38.0
38-39	34.45052754510309	37.0	34.0	38.0	24.5	38.0
40-41	35.257691688144334	38.0	35.5	38.0	29.0	38.0
42-43	35.64408827319588	38.0	36.0	38.0	31.0	38.0
44-45	35.634725354381445	38.0	36.0	38.0	31.0	38.0
46-47	35.64642396907217	38.0	36.0	38.0	31.0	38.0
48-49	35.64374597293815	38.0	36.0	38.0	31.0	38.0
50-51	35.50249677835052	38.0	36.0	38.0	31.0	38.0
52-53	35.58360180412372	38.0	36.0	38.0	31.0	38.0
54-55	35.48904639175258	38.0	36.0	38.0	31.0	38.0
56-57	35.46673646907217	38.0	36.0	38.0	31.0	38.0
58-59	35.33555492912372	38.0	36.0	38.0	30.0	38.0
60-61	35.343629188144334	38.0	36.0	38.0	30.0	38.0
62-63	35.3954574742268	38.0	36.0	38.0	30.5	38.0
64-65	35.46520618556701	38.0	36.0	38.0	31.0	38.0
66-67	35.433070231958766	38.0	36.0	38.0	31.0	38.0
68-69	34.898719394329895	38.0	35.0	38.0	27.0	38.0
70-71	33.69005718427835	37.0	33.5	38.0	22.5	38.0
72-73	32.13830943943299	37.0	29.5	38.0	11.0	38.0
74-75	31.845038659793815	37.0	27.5	38.0	11.0	38.0
76	31.0339481314433	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	3.0
22	4.0
23	8.0
24	21.0
25	42.0
26	96.0
27	137.0
28	268.0
29	461.0
30	655.0
31	970.0
32	1381.0
33	2017.0
34	3000.0
35	3987.0
36	4565.0
37	7216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.071681701030926	18.053318298969074	13.120167525773196	33.7548324742268
2	19.253382731958762	15.737757731958762	40.8666237113402	24.14223582474227
3	19.664143041237114	21.254832474226802	35.345521907216494	23.735502577319586
4	15.294780927835053	23.328769329896907	30.750644329896907	30.625805412371133
5	15.25853737113402	33.92799613402062	34.902545103092784	15.910921391752577
6	30.239207474226802	35.82876932989691	14.251771907216495	19.680251288659793
7	26.119523195876287	28.680734536082475	16.386114690721648	28.81362757731959
8	22.712628865979383	37.681217783505154	16.38208762886598	23.224065721649485
9	20.469555412371136	16.84519974226804	15.383376288659795	47.30186855670103
10-11	25.972535438144327	23.6730831185567	25.189271907216494	25.165109536082475
12-13	25.197326030927837	15.200144974226804	25.555734536082475	34.04679445876289
14-15	25.65641108247423	16.32973582474227	16.627738402061855	41.38611469072165
16-17	32.02118234536083	24.476481958762886	15.3894168814433	28.11291881443299
18-19	30.998308634020617	20.334648840206185	16.396182345360824	32.27086018041237
20-21	25.306056701030926	18.76610824742268	16.361952319587626	39.565882731958766
22-23	26.457796391752574	24.50265786082474	16.06394974226804	32.97559600515464
24-25	32.009101159793815	25.654397551546392	15.84447487113402	26.49202641752577
26-27	30.825144974226802	26.820231958762886	17.924452319587626	24.43017074742268
28-29	25.577883376288657	25.40270618556701	20.10107925257732	28.91833118556701
30-31	25.747019974226802	18.580863402061855	29.371375644329895	26.300740979381445
32-33	32.12588595360825	16.9519168814433	20.070876288659793	30.851320876288657
34-35	33.090367268041234	17.946601159793815	17.855992268041238	31.107039304123713
36-37	32.76014819587629	23.189835695876287	18.508376288659793	25.54163981958763
38-39	25.233569587628867	19.11243556701031	24.699983891752577	30.954010953608247
40-41	25.187258376288657	17.322406572164947	25.557748067010312	31.932586984536083
42-43	30.796955541237114	17.982844716494846	26.2866462628866	24.933553479381445
44-45	25.332232603092784	22.38442332474227	26.896746134020617	25.386597938144327
46-47	30.63990012886598	17.638530927835053	20.612516108247423	31.10905283505155
48-49	30.589561855670105	16.349871134020617	20.016510953608247	33.04405605670103
50-51	24.355670103092784	16.084085051546392	27.55114368556701	32.009101159793815
52-53	23.157619201030926	16.275370489690722	33.81523840206185	26.751771907216494
54-55	23.993234536082475	16.059922680412374	28.594152706185564	31.35269007731959
56-57	29.413659793814436	16.335776417525775	23.256282216494846	30.99428157216495
58-59	24.44225193298969	19.21311211340206	30.82715850515464	25.517477448453608
60-61	28.632409793814436	27.85921391752577	20.47962306701031	23.028753221649485
62-63	22.223340850515463	36.626127577319586	17.421069587628864	23.729461984536083
64-65	21.432023195876287	38.33561533505155	16.599548969072163	23.6328125
66-67	21.9897712628866	37.36509342783505	16.95997100515464	23.685164304123713
68-69	21.14207474226804	37.884584407216494	17.02440399484536	23.9489368556701
70-71	21.470280283505154	35.61130798969072	17.596246778350515	25.322164948453608
72-73	22.362274484536083	30.716414304123713	19.136597938144327	27.784713273195877
74-75	22.64819587628866	28.300177190721648	19.668170103092784	29.383456829896907
76	23.256282216494846	28.946520618556704	19.418492268041238	28.378704896907216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	2.5
20	6.5
21	9.0
22	7.5
23	8.5
24	14.5
25	18.0
26	26.0
27	41.0
28	46.5
29	45.0
30	60.5
31	78.5
32	110.0
33	139.0
34	145.5
35	166.5
36	212.0
37	243.0
38	279.5
39	345.0
40	498.0
41	1104.0
42	1586.0
43	1777.5
44	1830.0
45	1463.5
46	1236.0
47	1043.0
48	764.0
49	619.0
50	560.0
51	531.5
52	486.0
53	507.0
54	545.0
55	559.0
56	604.0
57	666.5
58	698.0
59	725.0
60	798.5
61	910.0
62	975.0
63	988.0
64	1008.0
65	1006.5
66	938.5
67	879.0
68	862.5
69	771.0
70	651.5
71	607.0
72	534.0
73	430.5
74	359.5
75	319.0
76	253.0
77	181.0
78	147.0
79	119.0
80	101.0
81	65.0
82	39.5
83	32.0
84	23.5
85	13.5
86	9.0
87	6.0
88	6.0
89	4.0
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	24832.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.9537693298969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4282475914615	50.797358247422686
2	15.13128896165229	19.354059278350515
3	3.557710471632769	6.825869845360824
4	0.9823059001322335	2.5128865979381443
5	0.3211384673509225	1.0269007731958764
6	0.1448271519425729	0.5557345360824743
7	0.10704615578364084	0.4792203608247423
8	0.03148416346577672	0.1610824742268041
9	0.01889049807946603	0.10873067010309279
>10	0.18890498079466028	1.9893685567010309
>50	0.03148416346577672	1.3611469072164948
>100	0.0440778288520874	5.368073453608247
>500	0.006296832693155343	2.1826675257731956
>1k	0.006296832693155343	7.276900773195877
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	1807	7.276900773195877	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	542	2.1826675257731956	RNA PCR Primer, Index 30 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	324	1.3047680412371134	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	258	1.0389819587628866	No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	233	0.938305412371134	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	208	0.8376288659793815	No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	106	0.4268685567010309	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	102	0.41076030927835055	No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT	102	0.41076030927835055	RNA PCR Primer, Index 30 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	87	0.350354381443299	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	76	0.30605670103092786	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	67	0.2698131443298969	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	57	0.2295425257731959	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	51	0.20538015463917528	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT	41	0.16510953608247422	No Hit
TCTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	35	0.1409471649484536	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT	35	0.1409471649484536	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG	30	0.1208118556701031	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.004027061855670103	0.0
6	0.0	0.0	0.0	0.004027061855670103	0.0
7	0.0	0.0	0.0	0.004027061855670103	0.0
8	0.0	0.0	0.0	0.004027061855670103	0.0
9	0.0	0.0	0.0	0.004027061855670103	0.0
10	0.0	0.0	0.0	0.004027061855670103	0.0
11	0.0	0.0	0.0	0.004027061855670103	0.0
12	0.0	0.0	0.0	0.004027061855670103	0.0
13	0.0	0.0	0.0	0.004027061855670103	0.0
14	0.0	0.0	0.0	0.008054123711340205	0.0
15	0.0	0.0	0.0	0.008054123711340205	0.0
16	0.0	0.0	0.0	0.008054123711340205	0.0
17	0.0	0.0	0.0	0.008054123711340205	0.0
18	0.0	0.0	0.0	0.008054123711340205	0.0
19	0.0	0.0	0.0	0.01208118556701031	0.0
20	0.0	0.0	0.0	0.01610824742268041	0.0
21	0.0	0.0	0.0	0.020135309278350517	0.0
22	0.0	0.0	0.0	0.02416237113402062	0.0
23	0.0	0.0	0.0	0.02416237113402062	0.0
24	0.0	0.0	0.0	0.02416237113402062	0.0
25	0.0	0.0	0.0	0.028189432989690722	0.0
26	0.0	0.0	0.0	0.028189432989690722	0.0
27	0.0	0.0	0.0	0.03624355670103093	0.0
28	0.0	0.0	0.0	0.04832474226804124	0.0
29	0.0	0.0	0.0	0.07248711340206186	0.0
30	0.0	0.0	0.0	0.08859536082474227	0.0
31	0.0	0.0	0.0	0.11275773195876289	0.0
32	0.0	0.0	0.0	0.11678479381443299	0.0
33	0.0	0.0	0.0	0.1328930412371134	0.0
34	0.0	0.0	0.0	0.19329896907216496	0.0
35	0.0	0.0	0.0	0.28994845360824745	0.0
36	0.0	0.0	0.0	0.41881443298969073	0.0
37	0.0	0.0	0.0	0.6523840206185567	0.0
38	0.0	0.0	0.0	0.9020618556701031	0.0
39	0.0	0.0	0.0	1.0711984536082475	0.0
40	0.0	0.0	0.0	1.2282538659793814	0.0
41	0.0	0.0	0.0	1.5504188144329898	0.0
42	0.0	0.0	0.0	1.755798969072165	0.0
43	0.0	0.0	0.0	2.037693298969072	0.0
44	0.0	0.0	0.0	2.3961018041237114	0.0
45	0.0	0.0	0.0	2.734375	0.0
46	0.0	0.0	0.0	3.12097293814433	0.0
47	0.0	0.0	0.0	3.4995167525773194	0.0
48	0.0	0.0	0.0	3.8176546391752577	0.0
49	0.0	0.0	0.0	4.172036082474227	0.0
50	0.0	0.0	0.0	4.558634020618556	0.0
51	0.0	0.0	0.0	4.808311855670103	0.0
52	0.0	0.0	0.0	5.08617912371134	0.0
53	0.0	0.0	0.0	5.392235824742268	0.0
54	0.0	0.0	0.0	5.734536082474227	0.0
55	0.0	0.0	0.0	6.048646907216495	0.0
56	0.0	0.0	0.0	6.334568298969073	0.0
57	0.0	0.0	0.0	6.66076030927835	0.0
58	0.0	0.0	0.0	7.05138530927835	0.0
59	0.0	0.0	0.0	7.373550257731959	0.0
60	0.0	0.0	0.0	7.740012886597938	0.0
61	0.0	0.0	0.0	8.078286082474227	0.0
62	0.0	0.0	0.0	8.360180412371134	0.0
63	0.0	0.0	0.0	8.710534793814434	0.0
64	0.0	0.0	0.0	9.016591494845361	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCATG	15	0.002199255	70.0	63
TTACACA	30	9.8354576E-8	70.0	3
ATTGCAG	15	0.002199255	70.0	26
GCTTGAC	20	7.776901E-5	70.0	56
CTTGACA	15	0.002199255	70.0	57
TCTTTAT	35	3.5179255E-9	70.0	1
CTTTACA	35	2.8581053E-7	60.0	1
TTTACAC	35	2.8581053E-7	60.0	2
AAACAAC	20	0.006872503	52.5	69
AACTCGT	20	0.006872503	52.5	37
CGGAACT	20	0.006872503	52.5	34
TTGACAA	20	0.006872503	52.5	58
CTTTATA	50	5.878246E-8	48.999996	2
TTTATAC	55	1.2444434E-7	44.545452	3
CGCTTGA	35	0.0012321657	40.0	55
CTATACA	45	8.9441295E-5	38.88889	1
GCCGGAA	40	0.002374231	35.0	32
CTTATAC	520	0.0	34.326923	1
GAAAAAA	520	0.0	33.653847	60
CCGTCTT	520	0.0	33.653847	47
>>END_MODULE